Mouse NKG2D/CD314 Biotinylated Antibody Summary
Phe94-Val232
Accession # Q4FJM0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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NKG2D/CD314 in Mouse Splenocytes. NKG2D/CD314 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse NKG2D/CD314 Biotinylated Monoclonal Antibody (Catalog # BAM1547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKG2D/CD314
NKG2D, also known as CD314, is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, δ, and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).
- Bauer, S. et al. (1999) Science 285:727.
- Wu, J. et al. (1999) Science 285:730.
- Diefenbach, A. et al. (2001) Nature 413:165.
- Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
- NKG2D and its Ligands; www.RnDSystems.com.
Product Datasheets
Citations for Mouse NKG2D/CD314 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts
Authors: Kurt Brassington, Peter Kanellakis, Anh Cao, Ban-Hock Toh, Karlheinz Peter, Alex Bobik et al.
Frontiers in Immunology
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Endogenous retinoids in the pathogenesis of alopecia areata.
Authors: Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H
J Invest Dermatol, 2012-09-27;133(2):334-43.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
NKG2D blockade prevents autoimmune diabetes in NOD mice.
Authors: Ogasawara K, Hamerman JA, Ehrlich LR, Bour-Jordan H, Santamaria P, Bluestone JA, Lanier LL
Immunity, 2004-06-01;20(6):757-67.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC
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