Mouse Myeloperoxidase DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Myeloperoxidase/MPO. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Scientific Data
Product Datasheets
Preparation and Storage
Background: Myeloperoxidase/MPO
Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Mouse Myeloperoxidase DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Gasdermin D promotes influenza virus-induced mortality through neutrophil amplification of inflammation
Authors: Speaks, S;McFadden, MI;Zani, A;Solstad, A;Leumi, S;Roettger, JE;Kenney, AD;Bone, H;Zhang, L;Denz, PJ;Eddy, AC;Amer, AO;Robinson, RT;Cai, C;Ma, J;Hemann, EA;Forero, A;Yount, JS;
Nature communications
Species: Murine polyomavirus strain A3
Sample Types: Cell Culture Supernates
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Heme sequestration by hemophilin from Haemophilus haemolyticus reduces respiratory tract colonization and infection with non-typeable Haemophilus influenzae
Authors: Fulte, S;Atto, B;McCarty, A;Horn, KJ;Redzic, JS;Eisenmesser, E;Yang, M;Marsh, RL;Tristram, S;Clark, SE;
mSphere
Species: Murine polyomavirus strain A3
Sample Types: Nasal Lavage Fluid, Bronchoalveolar Lavage Fluid
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Eosinophils preserve bone homeostasis by inhibiting excessive osteoclast formation and activity via eosinophil peroxidase
Authors: Andreev, D;Kachler, K;Liu, M;Chen, Z;Krishnacoumar, B;Ringer, M;Frey, S;Krönke, G;Voehringer, D;Schett, G;Bozec, A;
Nature communications
Species: Viral
Sample Types: Cell Culture Supernates
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NETs Promote Inflammatory Injury by Activating cGAS-STING Pathway in Acute Lung Injury
Authors: J Zhao, N Zhen, Q Zhou, J Lou, W Cui, G Zhang, B Tian
International Journal of Molecular Sciences, 2023-03-07;24(6):.
Species: Mouse
Sample Types: BALF
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ATG16L1 protects from interferon-gamma-induced cell death in the small intestinal crypt
Authors: EG Foerster, DKL Tsang, S Goyal, SJ Robertson, LM Robert, H Maughan, CJ Streutker, SE Girardin, DJ Philpott
Mucosal Immunology, 2023-02-14;0(0):.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
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A hierarchy of cell death pathways confers layered resistance to shigellosis in mice
Authors: JL Roncaioli, JP Babirye, RA Chavez, FL Liu, EA Turcotte, AY Lee, CF Lesser, RE Vance
Elife, 2023-01-16;12(0):.
Species: Mouse
Sample Types: Fecal Supernates
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Evaluation of Endocan as a Treatment for Acute Inflammatory Respiratory Failure
Authors: M Hureau, L Portier, M Prin, P de Nadai, J Balsamelli, A Tsicopoulo, D Mathieu, P Lassalle, B Grigoriu, A Gaudet, N De Freitas
Cells, 2023-01-07;12(2):.
Species: Mouse
Sample Types: BALF
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Oral commensal bacterial flora is responsible for peripheral differentiation of neutrophils in the oral mucosa in the steady state
Authors: N Trti?, M Mori, S Matsui, N Nakamura, H Fukamachi, M Kurosawa, H Morisaki, Y Maruoka, M Mayers, N Okahashi, H Kuwata, M Itsumi
Journal of oral biosciences, 2022-12-02;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Group B Streptococcus adaptation promotes survival in a hyperinflammatory diabetic wound environment
Authors: RA Keogh, AL Haeberle, CJ Langouët-A, JS Kavanaugh, EP Schmidt, GD Moore, AR Horswill, KS Doran
Science Advances, 2022-11-11;8(45):eadd3221.
Species: Mouse, Transgenic Mouse
Sample Types: Tissue Homogenates
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Efficacy of a Probiotic and Herbal Supplement in Models of Lung Inflammation
Authors: NM Wenger, L Qiao, T Nicola, Z Nizami, X Xu, KA Willis, N Ambalavana, A Gaggar, CV Lal
Microorganisms, 2022-10-28;10(11):.
Species: Mouse
Sample Types: BALF
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Nanoparticles reduce monocytes within the lungs to improve outcomes after influenza virus infection in aged mice
Authors: WJ Kelley, KM Wragg, J Chen, T Murthy, Q Xu, MT Boyne Ii, JR Podojil, A Elhofy, DR Goldstein
JCI Insight, 2022-08-08;0(0):.
Species: Mouse
Sample Types: BALF
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Nonhematopoietic IRAK1 drives arthritis via neutrophil chemoattractants
Authors: T Hoyler, B Bannert, C André, D Beck, T Boulay, D Buffet, N Caesar, T Calzascia, J Dawson, D Kyburz, R Hennze, C Huppertz, A Littlewood, P Loetscher, KD Mertz, S Niwa, G Robert, JS Rush, G Ruzzante, S Sarret, T Stein, I Touil, G Wieczorek, G Zipfel, S Hawtin, T Junt
JCI Insight, 2022-07-08;7(13):.
Species: Mouse
Sample Types: Tissue Homogenates
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ADAM8 signaling drives neutrophil migration and ARDS severity
Authors: C Conrad, D Yildiz, SJ Cleary, A Margraf, L Cook, U Schlomann, B Panaretou, JL Bowser, H Karmouty-Q, J Li, NK Berg, SC Martin, A Aljohmani, SF Moussavi-H, KM Wang, JJ Tian, M Magnen, C Valet, L Qiu, JP Singer, HK Eltzschig, CAPSys Stu, W Bertrams, S Herold, N Suttorp, B Schmeck, ZT Ball, A Zarbock, MR Looney, JW Bartsch
JCI Insight, 2022-02-08;7(3):.
Species: Mouse
Sample Types: BALF
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Gomisin M2 alleviates psoriasis?like skin inflammation by inhibiting inflammatory signaling pathways
Authors: N Kim, S Lee, J Kang, TK Kwon, D Khang, SH Kim
Molecular Medicine Reports, 2021-10-19;24(6):.
Species: Human
Sample Types: Serum
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Kaempferol Reduces Obesity, Prevents Intestinal Inflammation, and Modulates Gut Microbiota in High-fat Diet Mice: Kaempferol reduce inflammation and dysbacteria
Authors: Y Bian, J Lei, J Zhong, B Wang, Y Wan, J Li, C Liao, Y He, Z Liu, K Ito, B Zhang
The Journal of nutritional biochemistry, 2021-08-20;0(0):108840.
Species: Mouse
Sample Types: Tissue Homogenates
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Haematopoietic and cardiac GPR55 synchronize post-myocardial infarction remodelling
Authors: SL Puhl, M Hilby, M Kohlhaas, LM Keidel, Y Jansen, M Hristov, J Schindler, C Maack, S Steffens
Scientific Reports, 2021-07-13;11(1):14385.
Species: Mouse
Sample Types: Plasma
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Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus
Authors: A Doni, R Parente, I Laface, E Magrini, C Cunha, FS Colombo, JF Lacerda, A Campos, SN Mapelli, F Petroni, R Porte, T Schorn, A Inforzato, T Mercier, K Lagrou, J Maertens, JD Lambris, B Bottazzi, C Garlanda, M Botto, A Carvalho, A Mantovani
Nature Communications, 2021-06-18;12(1):3739.
Species: Mouse
Sample Types: BALF
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A high docosahexaenoic acid diet alters lung inflammation and recovery following repetitive exposure to aqueous organic dust extracts: Role of DHA in repetitive organic dust exposure
Authors: A Ulu, A Burr, AJ Heires, J Pavlik, T Larsen, PA Perez, C Bravo, NV DiPatrizio, M Baack, DJ Romberger, TM Nordgren
The Journal of nutritional biochemistry, 2021-06-12;0(0):108797.
Species: Mouse
Sample Types: BALF
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The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet
Authors: M Dupuit, V Chavanelle, B Chassaing, F Perriere, M Etienne, C Plissonnea, A Boscaro, N Barnich, V Pialoux, T Maugard, F Le Joubiou, S Peltier, P Sirvent, YF Otero, N Boisseau
Nutrients, 2021-05-07;13(5):.
Species: Rat
Sample Types: Plasma
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Pseudomonas aeruginosa-induced nociceptor activation increases susceptibility to infection
Authors: T Lin, D Quellier, J Lamb, T Voisin, P Baral, F Bock, A Schönberg, R Mirchev, G Pier, I Chiu, M Gadjeva
PloS Pathogens, 2021-05-06;17(5):e1009557.
Species: Mouse
Sample Types: Tissue Homogenates
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The anti-inflammatory cytokine interleukin-37 is an inhibitor of trained immunity
Authors: G Cavalli, IW Tengesdal, M Gresnigt, T Nemkov, RJW Arts, J Domínguez-, R Molteni, D Stefanoni, E Cantoni, L Cassina, S Giugliano, K Schraa, TS Mills, EM Pietras, EZ Eisenmenss, L Dagna, A Boletta, A D'Alessand, LAB Joosten, MG Netea, CA Dinarello
Cell Reports, 2021-04-06;35(1):108955.
Species: Mouse
Sample Types: Tissue Homogenates
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Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice
Authors: NV Bogatcheva, ME Coleman
Cells, 2021-03-24;10(4):.
Species: Mouse
Sample Types: BALF
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Prolyl Endopeptidase contributes to early neutrophilic inflammation in acute cardiac transplant rejection
Authors: GA Payne, NS Sharma, CV Lal, C Song, L Guo, C Margaroli, L Viera, S Kumar, J Li, M Bosley, D Xing, X Xu, JM Wells, JF George, JA Tallaj, M Leesar, JE Blalock, A Gaggar
JCI Insight, 2021-03-22;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Anthocyanin-containing Purple Potatoes Ameliorate DSS-induced Colitis in Mice
Authors: S Li, T Wang, B Wu, W Fu, B Xu, RR Pamuru, M Kennett, JKP Vanamala, L Reddivari
The Journal of nutritional biochemistry, 2021-03-08;0(0):108616.
Species: Mouse
Sample Types: Serum
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High-Intensity Interval Training and &alpha-Linolenic Acid Supplementation Improve DHA Conversion and Increase the Abundance of Gut Mucosa-Associated Oscillospira Bacteria
Authors: C Plissonnea, F Capel, B Chassaing, M Dupuit, F Maillard, I Wawrzyniak, L Combaret, F Dutheil, M Etienne, G Mairesse, G Chesneau, N Barnich, N Boisseau
Nutrients, 2021-02-27;13(3):.
Species: Rat
Sample Types: Plasma
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Elevated inflammatory responses and targeted therapeutic intervention in a preclinical mouse model of ataxia-telangiectasia lung disease
Authors: RA Saunders, TF Michniacki, C Hames, HA Moale, C Wilke, ME Kuo, J Nguyen, AJ Hartlerode, BB Moore, JM Sekiguchi
Scientific Reports, 2021-02-19;11(1):4268.
Species: Mouse
Sample Types: BALF
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Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model
Authors: H Park, S Yeo, S Kang, CS Huh
Microorganisms, 2021-02-12;9(2):.
Species: Mouse
Sample Types: Fecal Supernates
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Gasdermin D restricts Burkholderia cenocepacia infection in vitro and in vivo
Authors: S Estfanous, K Krause, MNK Anne, M Eltobgy, K Caution, A Abu Khweek, K Hamilton, A Badr, K Daily, C Carafice, D Baetzhold, X Zhang, T Li, H Wen, MA Gavrilin, H Haffez, S Soror, AO Amer
Scientific Reports, 2021-01-13;11(1):855.
Species: Mouse
Sample Types: Cell Culture Supernates
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COP9 signalosome is an essential and druggable parasite target that regulates protein degradation
Authors: S Ghosh, L Farr, A Singh, LA Leaton, J Padalia, DA Shirley, D Sullivan, S Moonah
PLoS Pathog., 2020-09-22;16(9):e1008952.
Species: Mouse
Sample Types: Tissue Homogenates
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Anti-Inflammatory and Gut Microbiota Modulatory Effect of Lactobacillus rhamnosus Strain LDTM 7511 in a Dextran Sulfate Sodium-Induced Colitis Murine Model
Authors: S Yeo, H Park, E Seo, J Kim, BK Kim, IS Choi, CS Huh
Microorganisms, 2020-06-04;8(6):.
Species: Mouse
Sample Types: Fecal Supernates
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Graphene quantum dots as anti-inflammatory therapy for colitis
Authors: BC Lee, JY Lee, J Kim, JM Yoo, I Kang, JJ Kim, N Shin, DJ Kim, SW Choi, D Kim, BH Hong, KS Kang
Sci Adv, 2020-04-29;6(18):eaaz2630.
Species: Mouse
Sample Types: Cell Culture Supernates
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Host Cathelicidin Exacerbates Group B Streptococcus Urinary Tract Infection
Authors: KA Patras, A Coady, P Babu, SR Shing, AD Ha, E Rooholfada, SL Brandt, M Geriak, RL Gallo, V Nizet
mSphere, 2020-04-22;5(2):.
Species: Mouse
Sample Types: Tissue Homogenate
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Intestinal Mucosal Barrier Function Restoration in Mice by Maize Diet Containing Enriched Flavan-4-Ols
Authors: B Wu, R Bhatnagar, VV Indukuri, S Chopra, K March, N Cordero, S Chopra, L Reddivari
Nutrients, 2020-03-25;12(4):.
Species: Mouse
Sample Types: Serum
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Nucleic Acid-Sensing Toll-Like Receptors Play a Dominant Role in Innate Immune Recognition of Pneumococci
Authors: A Famà, A Midiri, G Mancuso, C Biondo, G Lentini, R Galbo, MM Giardina, GV De Gaetano, L Romeo, G Teti, C Beninati
MBio, 2020-03-24;11(2):.
Species: Mouse
Sample Types: Tissue Lysates
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Biapenem as a Novel Insight into Drug Repositioning against Particulate Matter-Induced Lung Injury
Authors: W Lee, MC Baek, KM Kim, JS Bae
Int J Mol Sci, 2020-02-21;21(4):.
Species: Mouse
Sample Types: BALF
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Comparative Study on Pulmonary Toxicity in Mice Induced by Exposure to Unflavoured and Apple- and Strawberry-Flavoured Tobacco Waterpipe Smoke
Authors: A Nemmar, S Al-Salam, S Beegam, P Yuvaraju, BH Ali
Oxid Med Cell Longev, 2020-01-14;2020(0):6450450.
Species: Mouse
Sample Types: Tissue Homogenates
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Graphene quantum dots as anti-inflammatory therapy for colitis
Authors: BC Lee, JY Lee, J Kim, JM Yoo, I Kang, JJ Kim, N Shin, DJ Kim, SW Choi, D Kim, BH Hong, KS Kang
Sci Adv, 2020;6(18):eaaz2630.
Species: Mouse
Sample Types: Cell Culture Supernates
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PASylation of IL-1 Receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis
Authors: NE Powers, B Swartzwelt, C Marchetti, DM de Graaf, A Lerchner, M Schlapschy, R Datar, U Binder, CK Edwards, A Skerra, CA Dinarello
J. Biol. Chem., 2019-12-09;0(0):.
Species: Mouse
Sample Types: Peritoneal Fluid
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P2Y6 signaling in alveolar macrophages prevents leukotriene-dependent type 2 allergic lung inflammation
Authors: J Nagai, B Balestrier, LB Fanning, T Kyin, H Cirka, J Lin, M Idzko, A Zech, EY Kim, PJ Brennan, JA Boyce
J. Clin. Invest., 2019-12-02;0(0):.
Species: Mouse
Sample Types: BALF
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Intestinal autophagy links psychosocial stress with gut microbiota to promote inflammatory bowel disease
Authors: SL Wang, BZ Shao, SB Zhao, X Chang, P Wang, CY Miao, ZS Li, Y Bai
Cell Death Dis, 2019-09-30;10(6):391.
Species: Mouse
Sample Types: Tissue Homogenates
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Evidence of CD40L/CD40 pathway involvement in experimental transfusion-related acute lung injury
Authors: S Tariket, H Hamzeh-Cog, S Laradi, CA Arthaud, MA Eyraud, T Bourlet, P Berthelot, O Garraud, F Cognasse
Sci Rep, 2019-08-29;9(1):12536.
Species: Mouse
Sample Types: BALF
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Complement factor C5 inhibition reduces type 2 responses without affecting group 2 innate lymphoid cells in a house dust mite induced murine asthma model
Authors: J Yang, I Ramirez Mo, C van 't Vee, AF de Vos, R de Beer, JJTH Roelofs, BP Morgan, T van der Po
Respir. Res., 2019-07-24;20(1):165.
Species: Mouse
Sample Types: BALF
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Comparison of livestock-associated and community-associated Staphylococcus aureus pathogenicity in a mouse model of skin and soft tissue infection
Authors: PR Randad, CA Dillen, RV Ortines, D Mohr, M Aziz, LB Price, H Kaya, J Larsen, KC Carroll, TC Smith, LS Miller, CD Heaney
Sci Rep, 2019-05-01;9(1):6774.
Species: Mouse
Sample Types: Tissue Homogenates
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Gut microbiota from high-risk men who have sex with men drive immune activation in gnotobiotic mice and in vitro HIV infection
Authors: SX Li, S Sen, JM Schneider, KN Xiong, NM Nusbacher, N Moreno-Hui, M Shaffer, AJS Armstrong, E Severs, K Kuhn, CP Neff, M McCarter, T Campbell, CA Lozupone, BE Palmer
PLoS Pathog., 2019-04-04;15(4):e1007611.
Species: Mouse
Sample Types: Cell Lysates
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Long noncoding RNA Malat1 regulates differential activation of macrophage and response to lung injury
Authors: H Cui, S Banerjee, S Guo, N Xie, J Ge, D Jiang, M Zörnig, VJ Thannickal, G Liu
JCI Insight, 2019-02-21;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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GPER activation protects against epithelial barrier disruption by Staphylococcus aureus ?-toxin
Authors: KD Triplett, S Pokhrel, MJ Castleman, SM Daly, BO Elmore, JA Joyner, G Sharma, G Herbert, MJ Campen, HJ Hathaway, ER Prossnitz, PR Hall
Sci Rep, 2019-02-04;9(1):1343.
Species: Mouse
Sample Types: Tissue Homogenates
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PAD4-dependent NETs generation are indispensable for intestinal clearance of Citrobacter rodentium
Authors: P Saha, BS Yeoh, X Xiao, RM Golonka, V Singh, Y Wang, M Vijay-Kuma
Mucosal Immunol, 2019-02-01;0(0):.
Species: Mouse
Sample Types: Serum
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Myocardial Ischemia Reperfusion Injury: Apoptotic, Inflammatory and Oxidative Stress Role of Galectin-3
Authors: S Al-Salam, S Hashmi
Cell. Physiol. Biochem., 2018-10-24;50(3):1123-1139.
Species: Mouse
Sample Types: Tissue Homogenates
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Myeloperoxidase deficiency attenuates systemic and dietary iron-induced adverse effects
Authors: X Xiao, P Saha, BS Yeoh, JA Hipp, V Singh, M Vijay-Kuma
J. Nutr. Biochem., 2018-08-21;62(0):28-34.
Species: Mouse
Sample Types: Tissue Homogenates
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Metformin relieves acute respiratory distress syndrome by reducing miR-138 expression
Authors: LL Yu, M Zhu, Y Huang, YM Zhao, JJ Wen, XJ Yang, P Wu
Eur Rev Med Pharmacol Sci, 2018-08-01;22(16):5355-5363.
Species: Mouse
Sample Types: Tissue Homogenates
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Interleukin-22-deficiency and microbiota contribute to the exacerbation of Toxoplasma gondii-induced intestinal inflammation
Authors: A Couturier-, N Froux, J Piotet-Mor, C Michaudel, L Brault, J Le Bériche, A Sénéchal, P Robinet, P Chenuet, S Jejou, L Dumoutier, JC Renauld, J Iovanna, S Huber, V Quesniaux, H Sokol, B Ryffel
Mucosal Immunol, 2018-05-04;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Identification and Complete Stereochemical Assignments of the New Resolvin Conjugates in Tissue Regeneration in Human Tissues that Stimulate Proresolving Phagocyte Functions and Tissue Regeneration
Authors: X de la Rosa, PC Norris, N Chiang, AR Rodriguez, BW Spur, CN Serhan
Am. J. Pathol., 2018-04-01;188(4):950-966.
Species: Mouse
Sample Types: Tissue Homogenates
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Exosomal microRNA predicts and protects against severe bronchopulmonary dysplasia in extremely premature infants
Authors: CV Lal, N Olave, C Travers, G Rezonzew, K Dolma, A Simpson, B Halloran, Z Aghai, P Das, N Sharma, X Xu, K Genschmer, D Russell, T Szul, N Yi, JE Blalock, A Gaggar, V Bhandari, N Ambalavana
JCI Insight, 2018-03-08;3(5):.
Species: Mouse
Sample Types: BALF
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Mesenchymal Stromal Cells Inhibit Neutrophil Effector Functions in a Murine Model of Ocular Inflammation
Authors: SK Mittal, A Mashaghi, A Amouzegar, M Li, W Foulsham, SK Sahu, SK Chauhan
Invest. Ophthalmol. Vis. Sci., 2018-03-01;59(3):1191-1198.
Species: Mouse
Sample Types: Cell Culture Supernates
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Innate Sex Bias of Staphylococcus aureus Skin Infection Is Driven by ?-Hemolysin
Authors: MJ Castleman, S Pokhrel, KD Triplett, DF Kusewitt, BO Elmore, JA Joyner, JK Femling, G Sharma, HJ Hathaway, ER Prossnitz, PR Hall
J. Immunol., 2017-12-08;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Recombinant human IL-26 facilitates the innate immune response to endotoxin in the bronchoalveolar space of mice in vivo
Authors: A Bao, KF Che, S Bozinovski, J Ji, JA Gregory, S Kumlien Ge, M Adner, LO Cardell, A Lindén
PLoS ONE, 2017-12-05;12(12):e0188909.
Species: Mouse
Sample Types: BALF
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Isothiocyanate-enriched moringa seed extract alleviates ulcerative colitis symptoms in mice
Authors: Y Kim, AG Wu, A Jaja-Chime, BL Graf, C Waterman, MP Verzi, I Raskin
PLoS ONE, 2017-09-18;12(9):e0184709.
Species: Mouse
Sample Types: Cell Culture Supernates
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Lack of Histamine H4-Receptor Expression Aggravates TNBS-Induced Acute Colitis Symptoms in Mice
Authors: EJ Wunschel, B Schirmer, R Seifert, D Neumann
Front Pharmacol, 2017-09-13;8(0):642.
Species: Mouse
Sample Types: Tissue Homogenates
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Cross-modulation of pathogen-specific pathways enhances malnutrition during enteric co-infection with Giardia lamblia and enteroaggregative Escherichia coli
Authors: LA Bartelt, DT Bolick, J Mayneris-P, GL Kolling, GL Medlock, EI Zaenker, J Donowitz, RV Thomas-Bec, A Rogala, IM Carroll, SM Singer, J Papin, JR Swann, RL Guerrant
PLoS Pathog., 2017-07-27;13(7):e1006471.
Species: Mouse
Sample Types: Feces
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Dietary Salt Exacerbates Experimental Colitis
Authors: AL Tubbs, B Liu, TD Rogers, RB Sartor, EA Miao
J. Immunol., 2017-06-21;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Pterostilbene 4'-?-Glucoside Protects against DSS-Induced Colitis via Induction of Tristetraprolin
Authors: Y Chen, J Park, Y Joe, HJ Park, SJ Jekal, D Sato, H Hamada, HT Chung
Oxid Med Cell Longev, 2017-05-07;2017(0):9427583.
Species: Mouse
Sample Types: Tissue Homogenates
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Bacterial Siderophores Hijack Neutrophil Functions
Authors: P Saha, BS Yeoh, RA Olvera, X Xiao, V Singh, D Awasthi, BC Subramania, Q Chen, M Dikshit, Y Wang, CA Parent, M Vijay-Kuma
J. Immunol., 2017-04-21;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
Cell Death Dis, 2017-04-06;8(4):e2735.
Species: Mouse
Sample Types: Tissue Homogenates
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A�a� Berries Inhibit Colon Tumorigenesis in Azoxymethane/Dextran Sulfate Sodium-Treated Mice
Authors: Yoon Jin Choi
Gut Liver, 2017-03-15;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Corneal stromal stem cells reduce corneal scarring by mediating neutrophil infiltration after wounding
Authors: AJ Hertsenber, G Shojaati, ML Funderburg, MM Mann, Y Du, JL Funderburg
PLoS ONE, 2017-03-03;12(3):e0171712.
Species: Mouse
Sample Types: Whole Tissue
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Protective effects of ethyl pyruvate on lipopolysaccharide?induced acute lung injury through inhibition of autophagy in neutrophils
Authors: Q Zhu, H Wang, H Wang, Y Luo, Y Yu, Q Du, A Fei, S Pan
Mol Med Rep, 2017-01-13;15(3):1272-1278.
Species: Mouse
Sample Types: BALF
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Glaucocalyxin A improves survival in bleomycin-induced pulmonary fibrosis in mice
Authors: Li Zhu
Biochem. Biophys. Res. Commun., 2016-11-03;0(0):.
Species: Mouse
Sample Types: BALF
-
IL-1R1-MyD88 axis elicits papain-induced lung inflammation
Eur J Immunol, 2016-09-22;0(0):.
Species: Mouse
Sample Types: BALF
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Nox2-derived oxidative stress results in inefficacy of antibiotics against post-influenza S. aureus pneumonia
Authors: K Sun, VK Yajjala, C Bauer, GA Talmon, KJ Fischer, T Kielian, DW Metzger
J. Exp. Med, 2016-08-15;213(9):1851-64.
Species: Mouse
Sample Types: Tissue Homogenates
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Effects of a Soluble Epoxide Hydrolase Inhibitor on Lipopolysaccharide-Induced Acute Lung Injury in Mice
PLoS ONE, 2016-08-04;11(8):e0160359.
Species: Mouse
Sample Types: Tissue Homogenates
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Contribution of the Ade Resistance-Nodulation-Cell Division-Type Efflux Pumps to Fitness and Pathogenesis of Acinetobacter baumannii
Authors: Eun-Jeong Yoon
MBio, 2016-05-31;7(3):.
Species: Mouse
Sample Types: BALF
-
CD36 Is Essential for Regulation of the Host Innate Response to Staphylococcus aureus alpha-Toxin-Mediated Dermonecrosis.
Authors: Castleman M, Febbraio M, Hall P
J Immunol, 2015-07-29;195(5):2294-302.
Species: Mouse
Sample Types: Tissue Homogenates
-
Identification of resolvin D2 receptor mediating resolution of infections and organ protection.
Authors: Chiang N, Dalli J, Colas R, Serhan C
J Exp Med, 2015-07-20;212(8):1203-17.
Species: Mouse
Sample Types: Tissue Homogenates
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Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner.
Authors: Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R
Infect Immun, 2014-06-23;82(9):3723-39.
Species: Mouse
Sample Types: Tissue Homogenates
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Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na,K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury.
Authors: Wang Q, Zheng X, Cheng Y, Zhang Y, Wen H, Tao Z, Li H, Hao Y, Gao Y, Yang L, Smith F, Huang C, Jin S
J Immunol, 2014-03-19;192(8):3765-77.
Species: Rat
Sample Types: Tissue Homogenates
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Tristetraprolin mediates anti-inflammatory effect of carbon monoxide against DSS-induced colitis.
Authors: Joe Y, Uddin M, Zheng M, Kim H, Chen Y, Yoon N, Cho G, Park J, Chung H
PLoS ONE, 2014-02-19;9(2):e88776.
Species: Mouse
Sample Types: Tissue Homogenates
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Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis.
Authors: Hamers A, Uleman S, van Tiel C, Kruijswijk D, van Stalborch A, Huveneers S, de Vries C, van 't Veer C
Infect Immun, 2013-10-28;82(1):253-64.
Species: Mouse
Sample Types: Tissue Homogenates
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Average Rating: 4.3 (Based on 6 Reviews)
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Easy to use kit. Used for neutrophil supernatants. Good results, would use again.
Very stable to use, but OD of std increases after reconstitution. Higher STD loses linearity after a month of use.
The data is yet to be published and hence cannot give my graph.
Will give my data once it is published.