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Mouse Myeloperoxidase DuoSet ELISA

Catalog #: DY3667
77 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY3667
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
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Mouse Myeloperoxidase / MPO ELISA Standard Curve
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse Myeloperoxidase DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
250.0 - 16,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Myeloperoxidase/MPO. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse Myeloperoxidase / MPO ELISA Standard Curve View Larger

Mouse Myeloperoxidase / MPO ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.

Entrez Gene IDs:
4353 (Human); 17523 (Mouse); 303413 (Rat)
Alternate Names:
EC 1.11.1; EC 1.11.1.7; MPO; Myeloperoxidase

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse Myeloperoxidase DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

77 Citations: Showing 1 - 10
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  1. Gasdermin D promotes influenza virus-induced mortality through neutrophil amplification of inflammation
    Authors: Speaks, S;McFadden, MI;Zani, A;Solstad, A;Leumi, S;Roettger, JE;Kenney, AD;Bone, H;Zhang, L;Denz, PJ;Eddy, AC;Amer, AO;Robinson, RT;Cai, C;Ma, J;Hemann, EA;Forero, A;Yount, JS;
    Nature communications
    Species: Murine polyomavirus strain A3
    Sample Types: Cell Culture Supernates
  2. Heme sequestration by hemophilin from Haemophilus haemolyticus reduces respiratory tract colonization and infection with non-typeable Haemophilus influenzae
    Authors: Fulte, S;Atto, B;McCarty, A;Horn, KJ;Redzic, JS;Eisenmesser, E;Yang, M;Marsh, RL;Tristram, S;Clark, SE;
    mSphere
    Species: Murine polyomavirus strain A3
    Sample Types: Nasal Lavage Fluid, Bronchoalveolar Lavage Fluid
  3. Eosinophils preserve bone homeostasis by inhibiting excessive osteoclast formation and activity via eosinophil peroxidase
    Authors: Andreev, D;Kachler, K;Liu, M;Chen, Z;Krishnacoumar, B;Ringer, M;Frey, S;Krönke, G;Voehringer, D;Schett, G;Bozec, A;
    Nature communications
    Species: Viral
    Sample Types: Cell Culture Supernates
  4. NETs Promote Inflammatory Injury by Activating cGAS-STING Pathway in Acute Lung Injury
    Authors: J Zhao, N Zhen, Q Zhou, J Lou, W Cui, G Zhang, B Tian
    International Journal of Molecular Sciences, 2023-03-07;24(6):.
    Species: Mouse
    Sample Types: BALF
  5. ATG16L1 protects from interferon-gamma-induced cell death in the small intestinal crypt
    Authors: EG Foerster, DKL Tsang, S Goyal, SJ Robertson, LM Robert, H Maughan, CJ Streutker, SE Girardin, DJ Philpott
    Mucosal Immunology, 2023-02-14;0(0):.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
  6. A hierarchy of cell death pathways confers layered resistance to shigellosis in mice
    Authors: JL Roncaioli, JP Babirye, RA Chavez, FL Liu, EA Turcotte, AY Lee, CF Lesser, RE Vance
    Elife, 2023-01-16;12(0):.
    Species: Mouse
    Sample Types: Fecal Supernates
  7. Evaluation of Endocan as a Treatment for Acute Inflammatory Respiratory Failure
    Authors: M Hureau, L Portier, M Prin, P de Nadai, J Balsamelli, A Tsicopoulo, D Mathieu, P Lassalle, B Grigoriu, A Gaudet, N De Freitas
    Cells, 2023-01-07;12(2):.
    Species: Mouse
    Sample Types: BALF
  8. Oral commensal bacterial flora is responsible for peripheral differentiation of neutrophils in the oral mucosa in the steady state
    Authors: N Trti?, M Mori, S Matsui, N Nakamura, H Fukamachi, M Kurosawa, H Morisaki, Y Maruoka, M Mayers, N Okahashi, H Kuwata, M Itsumi
    Journal of oral biosciences, 2022-12-02;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Group B Streptococcus adaptation promotes survival in a hyperinflammatory diabetic wound environment
    Authors: RA Keogh, AL Haeberle, CJ Langouët-A, JS Kavanaugh, EP Schmidt, GD Moore, AR Horswill, KS Doran
    Science Advances, 2022-11-11;8(45):eadd3221.
    Species: Mouse, Transgenic Mouse
    Sample Types: Tissue Homogenates
  10. Efficacy of a Probiotic and Herbal Supplement in Models of Lung Inflammation
    Authors: NM Wenger, L Qiao, T Nicola, Z Nizami, X Xu, KA Willis, N Ambalavana, A Gaggar, CV Lal
    Microorganisms, 2022-10-28;10(11):.
    Species: Mouse
    Sample Types: BALF
  11. Nanoparticles reduce monocytes within the lungs to improve outcomes after influenza virus infection in aged mice
    Authors: WJ Kelley, KM Wragg, J Chen, T Murthy, Q Xu, MT Boyne Ii, JR Podojil, A Elhofy, DR Goldstein
    JCI Insight, 2022-08-08;0(0):.
    Species: Mouse
    Sample Types: BALF
  12. Nonhematopoietic IRAK1 drives arthritis via neutrophil chemoattractants
    Authors: T Hoyler, B Bannert, C André, D Beck, T Boulay, D Buffet, N Caesar, T Calzascia, J Dawson, D Kyburz, R Hennze, C Huppertz, A Littlewood, P Loetscher, KD Mertz, S Niwa, G Robert, JS Rush, G Ruzzante, S Sarret, T Stein, I Touil, G Wieczorek, G Zipfel, S Hawtin, T Junt
    JCI Insight, 2022-07-08;7(13):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. ADAM8 signaling drives neutrophil migration and ARDS severity
    Authors: C Conrad, D Yildiz, SJ Cleary, A Margraf, L Cook, U Schlomann, B Panaretou, JL Bowser, H Karmouty-Q, J Li, NK Berg, SC Martin, A Aljohmani, SF Moussavi-H, KM Wang, JJ Tian, M Magnen, C Valet, L Qiu, JP Singer, HK Eltzschig, CAPSys Stu, W Bertrams, S Herold, N Suttorp, B Schmeck, ZT Ball, A Zarbock, MR Looney, JW Bartsch
    JCI Insight, 2022-02-08;7(3):.
    Species: Mouse
    Sample Types: BALF
  14. Gomisin M2 alleviates psoriasis?like skin inflammation by inhibiting inflammatory signaling pathways
    Authors: N Kim, S Lee, J Kang, TK Kwon, D Khang, SH Kim
    Molecular Medicine Reports, 2021-10-19;24(6):.
    Species: Human
    Sample Types: Serum
  15. Kaempferol Reduces Obesity, Prevents Intestinal Inflammation, and Modulates Gut Microbiota in High-fat Diet Mice: Kaempferol reduce inflammation and dysbacteria
    Authors: Y Bian, J Lei, J Zhong, B Wang, Y Wan, J Li, C Liao, Y He, Z Liu, K Ito, B Zhang
    The Journal of nutritional biochemistry, 2021-08-20;0(0):108840.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. Haematopoietic and cardiac GPR55 synchronize post-myocardial infarction remodelling
    Authors: SL Puhl, M Hilby, M Kohlhaas, LM Keidel, Y Jansen, M Hristov, J Schindler, C Maack, S Steffens
    Scientific Reports, 2021-07-13;11(1):14385.
    Species: Mouse
    Sample Types: Plasma
  17. Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus
    Authors: A Doni, R Parente, I Laface, E Magrini, C Cunha, FS Colombo, JF Lacerda, A Campos, SN Mapelli, F Petroni, R Porte, T Schorn, A Inforzato, T Mercier, K Lagrou, J Maertens, JD Lambris, B Bottazzi, C Garlanda, M Botto, A Carvalho, A Mantovani
    Nature Communications, 2021-06-18;12(1):3739.
    Species: Mouse
    Sample Types: BALF
  18. A high docosahexaenoic acid diet alters lung inflammation and recovery following repetitive exposure to aqueous organic dust extracts: Role of DHA in repetitive organic dust exposure
    Authors: A Ulu, A Burr, AJ Heires, J Pavlik, T Larsen, PA Perez, C Bravo, NV DiPatrizio, M Baack, DJ Romberger, TM Nordgren
    The Journal of nutritional biochemistry, 2021-06-12;0(0):108797.
    Species: Mouse
    Sample Types: BALF
  19. The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet
    Authors: M Dupuit, V Chavanelle, B Chassaing, F Perriere, M Etienne, C Plissonnea, A Boscaro, N Barnich, V Pialoux, T Maugard, F Le Joubiou, S Peltier, P Sirvent, YF Otero, N Boisseau
    Nutrients, 2021-05-07;13(5):.
    Species: Rat
    Sample Types: Plasma
  20. Pseudomonas aeruginosa-induced nociceptor activation increases susceptibility to infection
    Authors: T Lin, D Quellier, J Lamb, T Voisin, P Baral, F Bock, A Schönberg, R Mirchev, G Pier, I Chiu, M Gadjeva
    PloS Pathogens, 2021-05-06;17(5):e1009557.
    Species: Mouse
    Sample Types: Tissue Homogenates
  21. The anti-inflammatory cytokine interleukin-37 is an inhibitor of trained immunity
    Authors: G Cavalli, IW Tengesdal, M Gresnigt, T Nemkov, RJW Arts, J Domínguez-, R Molteni, D Stefanoni, E Cantoni, L Cassina, S Giugliano, K Schraa, TS Mills, EM Pietras, EZ Eisenmenss, L Dagna, A Boletta, A D'Alessand, LAB Joosten, MG Netea, CA Dinarello
    Cell Reports, 2021-04-06;35(1):108955.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice
    Authors: NV Bogatcheva, ME Coleman
    Cells, 2021-03-24;10(4):.
    Species: Mouse
    Sample Types: BALF
  23. Prolyl Endopeptidase contributes to early neutrophilic inflammation in acute cardiac transplant rejection
    Authors: GA Payne, NS Sharma, CV Lal, C Song, L Guo, C Margaroli, L Viera, S Kumar, J Li, M Bosley, D Xing, X Xu, JM Wells, JF George, JA Tallaj, M Leesar, JE Blalock, A Gaggar
    JCI Insight, 2021-03-22;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. Anthocyanin-containing Purple Potatoes Ameliorate DSS-induced Colitis in Mice
    Authors: S Li, T Wang, B Wu, W Fu, B Xu, RR Pamuru, M Kennett, JKP Vanamala, L Reddivari
    The Journal of nutritional biochemistry, 2021-03-08;0(0):108616.
    Species: Mouse
    Sample Types: Serum
  25. High-Intensity Interval Training and &alpha-Linolenic Acid Supplementation Improve DHA Conversion and Increase the Abundance of Gut Mucosa-Associated Oscillospira Bacteria
    Authors: C Plissonnea, F Capel, B Chassaing, M Dupuit, F Maillard, I Wawrzyniak, L Combaret, F Dutheil, M Etienne, G Mairesse, G Chesneau, N Barnich, N Boisseau
    Nutrients, 2021-02-27;13(3):.
    Species: Rat
    Sample Types: Plasma
  26. Elevated inflammatory responses and targeted therapeutic intervention in a preclinical mouse model of ataxia-telangiectasia lung disease
    Authors: RA Saunders, TF Michniacki, C Hames, HA Moale, C Wilke, ME Kuo, J Nguyen, AJ Hartlerode, BB Moore, JM Sekiguchi
    Scientific Reports, 2021-02-19;11(1):4268.
    Species: Mouse
    Sample Types: BALF
  27. Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model
    Authors: H Park, S Yeo, S Kang, CS Huh
    Microorganisms, 2021-02-12;9(2):.
    Species: Mouse
    Sample Types: Fecal Supernates
  28. Gasdermin D restricts Burkholderia cenocepacia infection in vitro and in vivo
    Authors: S Estfanous, K Krause, MNK Anne, M Eltobgy, K Caution, A Abu Khweek, K Hamilton, A Badr, K Daily, C Carafice, D Baetzhold, X Zhang, T Li, H Wen, MA Gavrilin, H Haffez, S Soror, AO Amer
    Scientific Reports, 2021-01-13;11(1):855.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. COP9 signalosome is an essential and druggable parasite target that regulates protein degradation
    Authors: S Ghosh, L Farr, A Singh, LA Leaton, J Padalia, DA Shirley, D Sullivan, S Moonah
    PLoS Pathog., 2020-09-22;16(9):e1008952.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. Anti-Inflammatory and Gut Microbiota Modulatory Effect of Lactobacillus rhamnosus Strain LDTM 7511 in a Dextran Sulfate Sodium-Induced Colitis Murine Model
    Authors: S Yeo, H Park, E Seo, J Kim, BK Kim, IS Choi, CS Huh
    Microorganisms, 2020-06-04;8(6):.
    Species: Mouse
    Sample Types: Fecal Supernates
  31. Graphene quantum dots as anti-inflammatory therapy for colitis
    Authors: BC Lee, JY Lee, J Kim, JM Yoo, I Kang, JJ Kim, N Shin, DJ Kim, SW Choi, D Kim, BH Hong, KS Kang
    Sci Adv, 2020-04-29;6(18):eaaz2630.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. Host Cathelicidin Exacerbates Group B Streptococcus Urinary Tract Infection
    Authors: KA Patras, A Coady, P Babu, SR Shing, AD Ha, E Rooholfada, SL Brandt, M Geriak, RL Gallo, V Nizet
    mSphere, 2020-04-22;5(2):.
    Species: Mouse
    Sample Types: Tissue Homogenate
  33. Intestinal Mucosal Barrier Function Restoration in Mice by Maize Diet Containing Enriched Flavan-4-Ols
    Authors: B Wu, R Bhatnagar, VV Indukuri, S Chopra, K March, N Cordero, S Chopra, L Reddivari
    Nutrients, 2020-03-25;12(4):.
    Species: Mouse
    Sample Types: Serum
  34. Nucleic Acid-Sensing Toll-Like Receptors Play a Dominant Role in Innate Immune Recognition of Pneumococci
    Authors: A Famà, A Midiri, G Mancuso, C Biondo, G Lentini, R Galbo, MM Giardina, GV De Gaetano, L Romeo, G Teti, C Beninati
    MBio, 2020-03-24;11(2):.
    Species: Mouse
    Sample Types: Tissue Lysates
  35. Biapenem as a Novel Insight into Drug Repositioning against Particulate Matter-Induced Lung Injury
    Authors: W Lee, MC Baek, KM Kim, JS Bae
    Int J Mol Sci, 2020-02-21;21(4):.
    Species: Mouse
    Sample Types: BALF
  36. Comparative Study on Pulmonary Toxicity in Mice Induced by Exposure to Unflavoured and Apple- and Strawberry-Flavoured Tobacco Waterpipe Smoke
    Authors: A Nemmar, S Al-Salam, S Beegam, P Yuvaraju, BH Ali
    Oxid Med Cell Longev, 2020-01-14;2020(0):6450450.
    Species: Mouse
    Sample Types: Tissue Homogenates
  37. Graphene quantum dots as anti-inflammatory therapy for colitis
    Authors: BC Lee, JY Lee, J Kim, JM Yoo, I Kang, JJ Kim, N Shin, DJ Kim, SW Choi, D Kim, BH Hong, KS Kang
    Sci Adv, 2020;6(18):eaaz2630.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. PASylation of IL-1 Receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis
    Authors: NE Powers, B Swartzwelt, C Marchetti, DM de Graaf, A Lerchner, M Schlapschy, R Datar, U Binder, CK Edwards, A Skerra, CA Dinarello
    J. Biol. Chem., 2019-12-09;0(0):.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  39. P2Y6 signaling in alveolar macrophages prevents leukotriene-dependent type 2 allergic lung inflammation
    Authors: J Nagai, B Balestrier, LB Fanning, T Kyin, H Cirka, J Lin, M Idzko, A Zech, EY Kim, PJ Brennan, JA Boyce
    J. Clin. Invest., 2019-12-02;0(0):.
    Species: Mouse
    Sample Types: BALF
  40. Intestinal autophagy links psychosocial stress with gut microbiota to promote inflammatory bowel disease
    Authors: SL Wang, BZ Shao, SB Zhao, X Chang, P Wang, CY Miao, ZS Li, Y Bai
    Cell Death Dis, 2019-09-30;10(6):391.
    Species: Mouse
    Sample Types: Tissue Homogenates
  41. Evidence of CD40L/CD40 pathway involvement in experimental transfusion-related acute lung injury
    Authors: S Tariket, H Hamzeh-Cog, S Laradi, CA Arthaud, MA Eyraud, T Bourlet, P Berthelot, O Garraud, F Cognasse
    Sci Rep, 2019-08-29;9(1):12536.
    Species: Mouse
    Sample Types: BALF
  42. Complement factor C5 inhibition reduces type 2 responses without affecting group 2 innate lymphoid cells in a house dust mite induced murine asthma model
    Authors: J Yang, I Ramirez Mo, C van 't Vee, AF de Vos, R de Beer, JJTH Roelofs, BP Morgan, T van der Po
    Respir. Res., 2019-07-24;20(1):165.
    Species: Mouse
    Sample Types: BALF
  43. Comparison of livestock-associated and community-associated Staphylococcus aureus pathogenicity in a mouse model of skin and soft tissue infection
    Authors: PR Randad, CA Dillen, RV Ortines, D Mohr, M Aziz, LB Price, H Kaya, J Larsen, KC Carroll, TC Smith, LS Miller, CD Heaney
    Sci Rep, 2019-05-01;9(1):6774.
    Species: Mouse
    Sample Types: Tissue Homogenates
  44. Gut microbiota from high-risk men who have sex with men drive immune activation in gnotobiotic mice and in vitro HIV infection
    Authors: SX Li, S Sen, JM Schneider, KN Xiong, NM Nusbacher, N Moreno-Hui, M Shaffer, AJS Armstrong, E Severs, K Kuhn, CP Neff, M McCarter, T Campbell, CA Lozupone, BE Palmer
    PLoS Pathog., 2019-04-04;15(4):e1007611.
    Species: Mouse
    Sample Types: Cell Lysates
  45. Long noncoding RNA Malat1 regulates differential activation of macrophage and response to lung injury
    Authors: H Cui, S Banerjee, S Guo, N Xie, J Ge, D Jiang, M Zörnig, VJ Thannickal, G Liu
    JCI Insight, 2019-02-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  46. GPER activation protects against epithelial barrier disruption by Staphylococcus aureus ?-toxin
    Authors: KD Triplett, S Pokhrel, MJ Castleman, SM Daly, BO Elmore, JA Joyner, G Sharma, G Herbert, MJ Campen, HJ Hathaway, ER Prossnitz, PR Hall
    Sci Rep, 2019-02-04;9(1):1343.
    Species: Mouse
    Sample Types: Tissue Homogenates
  47. PAD4-dependent NETs generation are indispensable for intestinal clearance of Citrobacter rodentium
    Authors: P Saha, BS Yeoh, X Xiao, RM Golonka, V Singh, Y Wang, M Vijay-Kuma
    Mucosal Immunol, 2019-02-01;0(0):.
    Species: Mouse
    Sample Types: Serum
  48. Myocardial Ischemia Reperfusion Injury: Apoptotic, Inflammatory and Oxidative Stress Role of Galectin-3
    Authors: S Al-Salam, S Hashmi
    Cell. Physiol. Biochem., 2018-10-24;50(3):1123-1139.
    Species: Mouse
    Sample Types: Tissue Homogenates
  49. Myeloperoxidase deficiency attenuates systemic and dietary iron-induced adverse effects
    Authors: X Xiao, P Saha, BS Yeoh, JA Hipp, V Singh, M Vijay-Kuma
    J. Nutr. Biochem., 2018-08-21;62(0):28-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
  50. Metformin relieves acute respiratory distress syndrome by reducing miR-138 expression
    Authors: LL Yu, M Zhu, Y Huang, YM Zhao, JJ Wen, XJ Yang, P Wu
    Eur Rev Med Pharmacol Sci, 2018-08-01;22(16):5355-5363.
    Species: Mouse
    Sample Types: Tissue Homogenates
  51. Interleukin-22-deficiency and microbiota contribute to the exacerbation of Toxoplasma gondii-induced intestinal inflammation
    Authors: A Couturier-, N Froux, J Piotet-Mor, C Michaudel, L Brault, J Le Bériche, A Sénéchal, P Robinet, P Chenuet, S Jejou, L Dumoutier, JC Renauld, J Iovanna, S Huber, V Quesniaux, H Sokol, B Ryffel
    Mucosal Immunol, 2018-05-04;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  52. Identification and Complete Stereochemical Assignments of the New Resolvin Conjugates in Tissue Regeneration in Human Tissues that Stimulate Proresolving Phagocyte Functions and Tissue Regeneration
    Authors: X de la Rosa, PC Norris, N Chiang, AR Rodriguez, BW Spur, CN Serhan
    Am. J. Pathol., 2018-04-01;188(4):950-966.
    Species: Mouse
    Sample Types: Tissue Homogenates
  53. Exosomal microRNA predicts and protects against severe bronchopulmonary dysplasia in extremely premature infants
    Authors: CV Lal, N Olave, C Travers, G Rezonzew, K Dolma, A Simpson, B Halloran, Z Aghai, P Das, N Sharma, X Xu, K Genschmer, D Russell, T Szul, N Yi, JE Blalock, A Gaggar, V Bhandari, N Ambalavana
    JCI Insight, 2018-03-08;3(5):.
    Species: Mouse
    Sample Types: BALF
  54. Mesenchymal Stromal Cells Inhibit Neutrophil Effector Functions in a Murine Model of Ocular Inflammation
    Authors: SK Mittal, A Mashaghi, A Amouzegar, M Li, W Foulsham, SK Sahu, SK Chauhan
    Invest. Ophthalmol. Vis. Sci., 2018-03-01;59(3):1191-1198.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  55. Innate Sex Bias of Staphylococcus aureus Skin Infection Is Driven by ?-Hemolysin
    Authors: MJ Castleman, S Pokhrel, KD Triplett, DF Kusewitt, BO Elmore, JA Joyner, JK Femling, G Sharma, HJ Hathaway, ER Prossnitz, PR Hall
    J. Immunol., 2017-12-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  56. Recombinant human IL-26 facilitates the innate immune response to endotoxin in the bronchoalveolar space of mice in vivo
    Authors: A Bao, KF Che, S Bozinovski, J Ji, JA Gregory, S Kumlien Ge, M Adner, LO Cardell, A Lindén
    PLoS ONE, 2017-12-05;12(12):e0188909.
    Species: Mouse
    Sample Types: BALF
  57. Isothiocyanate-enriched moringa seed extract alleviates ulcerative colitis symptoms in mice
    Authors: Y Kim, AG Wu, A Jaja-Chime, BL Graf, C Waterman, MP Verzi, I Raskin
    PLoS ONE, 2017-09-18;12(9):e0184709.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  58. Lack of Histamine H4-Receptor Expression Aggravates TNBS-Induced Acute Colitis Symptoms in Mice
    Authors: EJ Wunschel, B Schirmer, R Seifert, D Neumann
    Front Pharmacol, 2017-09-13;8(0):642.
    Species: Mouse
    Sample Types: Tissue Homogenates
  59. Cross-modulation of pathogen-specific pathways enhances malnutrition during enteric co-infection with Giardia lamblia and enteroaggregative Escherichia coli
    Authors: LA Bartelt, DT Bolick, J Mayneris-P, GL Kolling, GL Medlock, EI Zaenker, J Donowitz, RV Thomas-Bec, A Rogala, IM Carroll, SM Singer, J Papin, JR Swann, RL Guerrant
    PLoS Pathog., 2017-07-27;13(7):e1006471.
    Species: Mouse
    Sample Types: Feces
  60. Dietary Salt Exacerbates Experimental Colitis
    Authors: AL Tubbs, B Liu, TD Rogers, RB Sartor, EA Miao
    J. Immunol., 2017-06-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  61. Pterostilbene 4'-?-Glucoside Protects against DSS-Induced Colitis via Induction of Tristetraprolin
    Authors: Y Chen, J Park, Y Joe, HJ Park, SJ Jekal, D Sato, H Hamada, HT Chung
    Oxid Med Cell Longev, 2017-05-07;2017(0):9427583.
    Species: Mouse
    Sample Types: Tissue Homogenates
  62. Bacterial Siderophores Hijack Neutrophil Functions
    Authors: P Saha, BS Yeoh, RA Olvera, X Xiao, V Singh, D Awasthi, BC Subramania, Q Chen, M Dikshit, Y Wang, CA Parent, M Vijay-Kuma
    J. Immunol., 2017-04-21;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  63. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017-04-06;8(4):e2735.
    Species: Mouse
    Sample Types: Tissue Homogenates
  64. A�a� Berries Inhibit Colon Tumorigenesis in Azoxymethane/Dextran Sulfate Sodium-Treated Mice
    Authors: Yoon Jin Choi
    Gut Liver, 2017-03-15;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  65. Corneal stromal stem cells reduce corneal scarring by mediating neutrophil infiltration after wounding
    Authors: AJ Hertsenber, G Shojaati, ML Funderburg, MM Mann, Y Du, JL Funderburg
    PLoS ONE, 2017-03-03;12(3):e0171712.
    Species: Mouse
    Sample Types: Whole Tissue
  66. Protective effects of ethyl pyruvate on lipopolysaccharide?induced acute lung injury through inhibition of autophagy in neutrophils
    Authors: Q Zhu, H Wang, H Wang, Y Luo, Y Yu, Q Du, A Fei, S Pan
    Mol Med Rep, 2017-01-13;15(3):1272-1278.
    Species: Mouse
    Sample Types: BALF
  67. Glaucocalyxin A improves survival in bleomycin-induced pulmonary fibrosis in mice
    Authors: Li Zhu
    Biochem. Biophys. Res. Commun., 2016-11-03;0(0):.
    Species: Mouse
    Sample Types: BALF
  68. IL-1R1-MyD88 axis elicits papain-induced lung inflammation
    Eur J Immunol, 2016-09-22;0(0):.
    Species: Mouse
    Sample Types: BALF
  69. Nox2-derived oxidative stress results in inefficacy of antibiotics against post-influenza S. aureus pneumonia
    Authors: K Sun, VK Yajjala, C Bauer, GA Talmon, KJ Fischer, T Kielian, DW Metzger
    J. Exp. Med, 2016-08-15;213(9):1851-64.
    Species: Mouse
    Sample Types: Tissue Homogenates
  70. Effects of a Soluble Epoxide Hydrolase Inhibitor on Lipopolysaccharide-Induced Acute Lung Injury in Mice
    PLoS ONE, 2016-08-04;11(8):e0160359.
    Species: Mouse
    Sample Types: Tissue Homogenates
  71. Contribution of the Ade Resistance-Nodulation-Cell Division-Type Efflux Pumps to Fitness and Pathogenesis of Acinetobacter baumannii
    Authors: Eun-Jeong Yoon
    MBio, 2016-05-31;7(3):.
    Species: Mouse
    Sample Types: BALF
  72. CD36 Is Essential for Regulation of the Host Innate Response to Staphylococcus aureus alpha-Toxin-Mediated Dermonecrosis.
    Authors: Castleman M, Febbraio M, Hall P
    J Immunol, 2015-07-29;195(5):2294-302.
    Species: Mouse
    Sample Types: Tissue Homogenates
  73. Identification of resolvin D2 receptor mediating resolution of infections and organ protection.
    Authors: Chiang N, Dalli J, Colas R, Serhan C
    J Exp Med, 2015-07-20;212(8):1203-17.
    Species: Mouse
    Sample Types: Tissue Homogenates
  74. Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner.
    Authors: Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R
    Infect Immun, 2014-06-23;82(9):3723-39.
    Species: Mouse
    Sample Types: Tissue Homogenates
  75. Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na,K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury.
    Authors: Wang Q, Zheng X, Cheng Y, Zhang Y, Wen H, Tao Z, Li H, Hao Y, Gao Y, Yang L, Smith F, Huang C, Jin S
    J Immunol, 2014-03-19;192(8):3765-77.
    Species: Rat
    Sample Types: Tissue Homogenates
  76. Tristetraprolin mediates anti-inflammatory effect of carbon monoxide against DSS-induced colitis.
    Authors: Joe Y, Uddin M, Zheng M, Kim H, Chen Y, Yoon N, Cho G, Park J, Chung H
    PLoS ONE, 2014-02-19;9(2):e88776.
    Species: Mouse
    Sample Types: Tissue Homogenates
  77. Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis.
    Authors: Hamers A, Uleman S, van Tiel C, Kruijswijk D, van Stalborch A, Huveneers S, de Vries C, van 't Veer C
    Infect Immun, 2013-10-28;82(1):253-64.
    Species: Mouse
    Sample Types: Tissue Homogenates

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Reviews for Mouse Myeloperoxidase DuoSet ELISA

Average Rating: 4.3 (Based on 6 Reviews)

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Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 04/22/2022
Sample Tested: Cell culture supernatant

Easy to use kit. Used for neutrophil supernatants. Good results, would use again.

Mouse Myeloperoxidase DuoSet ELISA DY3667
Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 01/04/2022
Sample Tested: Colon tissue

Very stable to use, but OD of std increases after reconstitution. Higher STD loses linearity after a month of use.

Mouse Myeloperoxidase DuoSet ELISA DY3667
Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 06/08/2017
Sample Tested: Lung tissue
Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 06/08/2017
Sample Tested: Lung tissue
Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 04/21/2017
Sample Tested: Colon tissue
Mouse Myeloperoxidase DuoSet ELISA
By Anonymous on 06/02/2016
Sample Tested: Adult lung

The data is yet to be published and hence cannot give my graph.

Will give my data once it is published.