Mouse LYVE-1 Biotinylated Antibody

Detection of Mouse LYVE-1 by Immunohistochemistry TLS formation in models of heart failure, conditional lymphatic-EC deletion of Rbpj or kidney ischemia reperfusion. D PAS staining of representative, paraffin-embedded kidney sections. Quantification of infiltrated area [in mm2] per transversal kidney cross-section (sum of all infiltrated areas per section), N = 8 biological replicates per group, Mann–Whitney test, two-tailed, p = 0.51. Graph: Scatter dot blot, Mean, SD (whiskers). ERbpj delta EC Whole kidney staining for B220 (TLS)&Prox1/Lyve1 for lymphatic collecting vessels, light sheet microscopy, ventral view, 3D reconstruction via IMARIS software; scale bar: left image 1000 µm; insets are magnifications of boxed detail, scale bar 150 µm. Exemplary image, kidneys from N = 3 mice stained. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35440634), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse LYVE-1 by Immunohistochemistry Lack of Wnt ligand secretion from LSECs impairs adult zonation maintenance.(A) Double in situ hybridization for Rspo3 (green), Wnt9b (green)&Wnt2 (green) showing a few LSCEs (Lyve1+, red) expressing those transcripts (blue arrows&inset) in P2 livers. Only Wnt2 transcripts are detected in some LSECs (inset) in P60 livers. Arrows indicate central vein endothelial cells&arrowheads indicate LSECs. Scale bars: 25 μm. Each image is representative of 3 individual mice (n = 3). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32154783), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse LYVE-1 by Immunohistochemistry Lack of Wnt ligand secretion from LSECs impairs adult zonation maintenance. (B) Double-immunofluorescence results show that hepatic Zones 3 (GS+)&2/3 (Cyp2e1+) are densely irrigated by the hepatic sinusoids (Lyve1+, arrows) in P2, P15&P30 wildtype livers. The sinusoidal vasculature (arrows) is also in direct contact with claudin-2/GFP+ hepatocytes in P2, P15&P30 Cldn2-EGFP livers. Scale bars: 50 μm. Each image is representative of 2–4 individual mice (n = 2–4). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32154783), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse LYVE-1 by Immunohistochemistry Lineage tracing results demonstrate selective beta -gal expression in LSECs in Lyve1-Cre;ROSA-LacZ livers.(A, B) Immunofluorescence results showing expression of the lineage tracer beta -gal in PECAM+ endothelial cells connecting to the central vein (A, yellow arrows) and in parenchymal PECAM+/Lyve1+ LSECs (white arrows in (A and B). beta -gal is not expressed in PECAM+ central vein endothelial cells (A, arrowhead; the central vein is surrounded by GS+ hepatocytes). Images are representative of 2 individual livers. Scale bars: 25 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32154783), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse LYVE-1 by Immunohistochemistry Molecular, cellular and structural composition of periarterial TLS.A–I Immunofluorescence staining and confocal laser scanning microscopy of representative Rbpj delta EC kidney samples, merged and single channels as indicated. “A” indicates artery. Optical Magnification 200x; different scan areas (see scale bars). Scale: solid bar 50 µm, dotted bar 10 µm (2E). Each micrograph is representative of at least 4 biological replicates. J Whole kidney mRNA expression, relative fold change to control gene Rps9, n = 10/group. Graphs: Scatter dot blot, mean, SD (whiskers). Mann–Whitney test, two-tailed, Exact p-values: Cxcl13, p = 0.0003; Cxcl12, p = 0.393; Cxcr5, p = 0.0433; Cxcr4, p = 0.0288; Ccl19, p = 0.0052; Baff, p = 0.0007; Rankl, p = 0.0005. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35440634), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse LYVE-1 by Immunohistochemistry Lyve1-Cre;Wlsf/f mice are refractory to CCl4-induced hepatotoxicity.(A) (Left) Schematic of CCl4 administration&tissue harvesting using Cldn2-EGFP mice. (Right) Double-immunofluorescence results show that claudin-2/GFP+ hepatocytes (arrows) are in close proximity to the sinusoidal endothelium (Lyve1+, arrows) throughout the recovery period that follows CCl4 acute injury. (B) GS (Zone 3), Cyp2e1 (Zone 2/3)&E-cadherin (Zone 1) expression in control&Lyve1-Cre;Wlsf/f livers without CCl4 treatment. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32154783), licensed under a CC-BY license. Not internally tested by R&D Systems.