Home / Leptin R / Mouse Leptin R Antibody

Mouse Leptin R Antibody

Catalog #: AF497
44 Citations 2 Reviews Datasheet / COA / SDS
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AF497
AF497-SP
Leptin R in Rat Brain.
2 Images
Product Details
Citations (44)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Leptin R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Leptin R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
Ala20-Gly839
Accession # Q3US58
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR)
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Leptin R antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

Leptin R in Rat Brain. Leptin R was detected in perfusion fixed frozen sections of rat brain using 15 µg/mL Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the choroid plexus. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Human Leptin R by Western Blot View Larger

Detection of Human Leptin R by Western Blot Expression and isolation of recombinant murine LepRec-Fc fusion proteins.a) Expression of murine LepRec-Fc constructs in adherent HEK293T/17 cells. Supernatants containing LepRec-Fc chimera proteins from 24 and 48 hours post-transfection were cleared of cellular debris, subjected to SDS PAGE and western blotted with antibodies against domains of murine IgG1 (Fc specific for lanes 1–5 and Ab specific for lanes 6–8). Lane 1: Mock transfected at 48 hours. Lane 2: WT transfected at 24 hours. Lane 3: WT transfected at 48 hours. Lane 4: Q223R transfected at 24 hours. Lane 5: Q223R transfected at 48 hours. Lane 6: Mock transfected at 48 hours. Lane 7: WT transfected at 48 hours. Lane 8: Q223R transfected at 48 hours. b) Concentration and buffer exchange of murine LepRec-Fc chimeras. Supernatants were collected 48 hours after growth medium was replaced with expression medium (serum free Optimem +2 mM sodium butyrate). These supernatant preparations were concentrated by Amicon ultrafiltration (NMWCO of 100 kDa). Supernatants, concentrates, and filtrates from each chimera were subjected to SDS-PAGE followed by coomassie staining (top) and western blotting with alpha -murine IgG1 specific to the Fc region (bottom). Lane 1: Expression medium. Lane 2: WT supernatant. Lane 3: WT concentrate. Lane 4: WT filtrate. Lane 5: Q223R supernatant. Lane 6: Q223R Concentrate. Lane 7: Q223R Filtrate. c) Supernatants, concentrates, and filtrates (as in b) from each chimera were subjected to SDS-PAGE followed by transfer to PVDF membrane and western blotting with alpha -murine leptin receptor (R&D scientific). Lane 1: Expression medium. Lane 2: WT supernatant. Lane 3: WT concentrate. Lane 4: WT filtrate. Lane 5: Q223R Filtrate. Lane 6: Q223R Concentrate. Lane 7: Q223R supernatant. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24743494), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

References
  1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  3. Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
  4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
  5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.
Long Name
Leptin Receptor
Entrez Gene IDs
3953 (Human); 16847 (Mouse)
Alternate Names
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295

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Citations for Mouse Leptin R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues
    Authors: Mehmet Saçma, Francesca Matteini, Medhanie A. Mulaw, Ali Hageb, Ruzhica Bogeska, Vadim Sakk et al.
    STAR Protocols
  2. A novel compound heterozygous leptin receptor mutation causes more severe obesity than in Leprdb/db mice
    Authors: Claudia Berger, Henrike O. Heyne, Tina Heiland, Sebastian Dommel, Corinna Höfling, Esther Guiu-Jurado et al.
    Journal of Lipid Research
  3. Receptor-mediated dimerization of JAK2 FERM domains is required for JAK2 activation
    Authors: Ryan D Ferrao, Heidi JA Wallweber, Patrick J Lupardus
    eLife
  4. Gs alpha R201C and estrogen reveal different subsets of bone marrow adiponectin expressing osteogenic cells
    Authors: Biagio Palmisano, Rossella Labella, Samantha Donsante, Cristina Remoli, Emanuela Spica, Ilenia Coletta et al.
    Bone Research
  5. Oxidized phospholipids are ligands for LRP6
    Authors: Lei Wang, Yu Chai, Changjun Li, Haiyun Liu, Weiping Su, Xiaonan Liu et al.
    Bone Research
  6. Correction--JAK/STAT3-Regulated Fatty Acid beta -Oxidation Is Critical for Breast Cancer Stem Cell Self-Renewal and Chemoresistance
    Authors: Tianyi Wang, Johannes Francois Fahrmann, Heehyoung Lee, Yi-Jia Li, Satyendra C. Tripathi, Chanyu Yue et al.
    Cell Metabolism
  7. Multiparametric senescent cell phenotyping reveals CD24 osteolineage cells as targets of senolytic therapy in the aged murine skeleton
    Authors: Madison L. Doolittle, Dominik Saul, Japneet Kaur, Jennifer L. Rowsey, Stephanie J. Vos, Kevin D. Pavelko et al.
    bioRxiv
  8. CB2 receptor deletion on myeloid cells enhanced mechanical allodynia in a mouse model of neuropathic pain
    Authors: Elisa Nent, Chihiro Nozaki, Anne-Caroline Schmöle, David Otte, Andreas Zimmer
    Scientific Reports
  9. Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment
    Authors: Leilei Zhong, Lutian Yao, Robert J Tower, Yulong Wei, Zhen Miao, Jihwan Park et al.
    eLife
  10. Mdm2/p53 levels in bone marrow mesenchymal stromal cells is essential for maintaining the hematopoietic niche in response to DNA damage
    Authors: R Pourebrahi, RH Montoya, Z Alaniz, L Ostermann, PP Lin, B Liu, E Ayoub, JK Burks, M Andreeff
    Research square, 2023-03-02;0(0):.
  11. Uptake of ox-LDL by binding to LRP6 mediates oxidative stress-induced BMSCs senescence promoting obesity-related bone loss
    Authors: Meng, S;Wang, Z;Liu, X;Shen, K;Gu, Y;Yu, B;Wang, L;
    Cellular signalling
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Mdm2/p53 levels in bone marrow mesenchymal stromal cells are essential for maintaining the hematopoietic niche in response to DNA damage
    Authors: Pourebrahim, R;Heinz Montoya, R;Alaniz, Z;Ostermann, L;Lin, PP;Liu, B;Ayoub, E;Burks, JK;Andreeff, M;
    Cell death & disease
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Bone metastasis initiation is coupled with bone remodeling through osteogenic differentiation of NG2+ cells
    Authors: W Zhang, Z Xu, X Hao, T He, J Li, Y Shen, K Liu, Y Gao, J Liu, D Edwards, AM Muscarella, L Wu, L Yu, L Xu, X Chen, YH Wu, IL Bado, Y Ding, S Aguirre, H Wang, Z Gugala, RL Satcher, ST Wong, XH Zhang
    Cancer Discovery, 2023-02-06;0(0):.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Ras drives malignancy through stem cell crosstalk with the microenvironment
    Authors: S Yuan, KS Stewart, Y Yang, MD Abdusselam, SM Parigi, TY Feinberg, K Tumaneng, H Yang, JM Levorse, L Polak, D Ng, E Fuchs
    Nature, 2022-11-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
    Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
    Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  16. Primary cilia mediate early life programming of adiposity through lysosomal regulation in the developing mouse hypothalamus
    Authors: CH Lee, DK Song, CB Park, J Choi, GM Kang, SH Shin, I Kwon, S Park, S Kim, JY Kim, H Dugu, JW Park, JH Choi, SH Min, JW Sohn, MS Kim
    Nat Commun, 2020-11-13;11(1):5772.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Chronic myelogenous leukemia stem cells require cell-autonomous pleiotrophin signaling
    Authors: HA Himburg, M Roos, T Fang, Y Zhang, CM Termini, L Schlussel, MM Kim, A Pang, J Kan, L Zhao, H Suh, JP Sasine, G Sapparapu, PM Bowers, G Schiller, JP Chute
    J. Clin. Invest., 2020-01-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Haematopoietic stem cells in perisinusoidal niches are protected from ageing
    Authors: M Saçma, J Pospiech, R Bogeska, W de Back, JP Mallm, V Sakk, K Soller, G Marka, A Vollmer, R Karns, N Cabezas-Wa, A Trumpp, S Méndez-Fer, MD Milsom, MA Mulaw, H Geiger, MC Florian
    Nat. Cell Biol., 2019-11-04;21(11):1309-1320.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping
    Authors: N Severe, NM Karabacak, K Gustafsson, N Baryawno, G Courties, Y Kfoury, KD Kokkaliari, C Rhee, D Lee, EW Scadden, JE Garcia-Rob, T Brouse, M Nahrendorf, M Toner, DT Scadden
    Cell Stem Cell, 2019-07-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. A spontaneous leptin receptor point mutation causes obesity and differentially affects leptin signaling in hypothalamic nuclei resulting in metabolic dysfunctions distinct from db/db mice
    Authors: F Piattini, C Le Foll, J Kisielow, E Rosenwald, P Nielsen, T Lutz, C Schneider, M Kopf
    Mol Metab, 2019-04-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B
    Authors: BJ Frisch, CM Hoffman, SE Latchney, MW LaMere, J Myers, J Ashton, AJ Li, J Saunders, J Palis, AS Perkins, A McCabe, JN Smith, KE McGrath, F Rivera-Esc, A McDavid, JL Liesveld, VA Korshunov, MR Elliott, KC MacNamara, MW Becker, LM Calvi
    JCI Insight, 2019-04-18;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain
    Authors: C Nozaki, E Nent, A Bilkei-Gor, A Zimmer
    Sci Rep, 2018-07-17;8(1):10827.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Aberrant TGF-? activation in bone tendon insertion induces enthesopathy-like disease
    Authors: X Wang, L Xie, J Crane, G Zhen, F Li, P Yang, M Gao, R Deng, Y Wang, X Jia, C Fan, M Wan, X Cao
    J. Clin. Invest., 2018-01-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Programmed cell senescence in skeleton during late puberty
    Authors: C Li, Y Chai, L Wang, B Gao, H Chen, P Gao, FQ Zhou, X Luo, JL Crane, B Yu, X Cao, M Wan
    Nat Commun, 2017-11-03;8(1):1312.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  26. Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion
    Authors: KT Chang, YL Lin, CT Lin, MJ Tsai, WC Huang, YH Shih, YY Lee, H Cheng, MC Huang
    Data Brief, 2017-10-07;15(0):567-572.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. A dangerous liaison: Leptin and sPLA2-IIA join forces to induce proliferation and migration of astrocytoma cells
    Authors: R Martín, C Cordova, B Gutiérrez, M Hernández, ML Nieto
    PLoS ONE, 2017-03-01;12(3):e0170675.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Differential cytokine contributions of perivascular haematopoietic stem cell niches
    Authors: N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette
    Nat. Cell Biol, 2017-02-20;19(3):214-223.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Hypomorphism for RPGRIP1L, a ciliary gene vicinal to the FTO locus, causes increased adiposity in mice.
    Authors: Stratigopoulos G, Martin Carli J, O'Day D, Wang L, Leduc C, Lanzano P, Chung W, Rosenbaum M, Egli D, Doherty D, Leibel R
    Cell Metab, 2014-05-06;19(5):767-79.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Immunoprecipitation
  30. Kinetics of leptin binding to the Q223R leptin receptor.
    Authors: Verkerke H, Naylor C, Zabeau L, Tavernier J, Petri W, Marie C
    PLoS ONE, 2014-04-17;9(4):e94843.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  31. Deletion of selenoprotein M leads to obesity without cognitive deficits.
    Authors: Pitts, Matthew, Reeves, Mariclai, Hashimoto, Ann C, Ogawa, Ashley, Kremer, Penny, Seale, Lucia A, Berry, Marla J
    J Biol Chem, 2013-07-23;288(36):26121-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  32. Leptin enhances CC-chemokine ligand expression in cultured murine macrophage.
    Authors: Kiguchi N, Maeda T, Kobayashi Y, Fukazawa Y, Kishioka S
    Biochem. Biophys. Res. Commun., 2009-05-03;384(3):311-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  33. Soluble receptor inhibits leptin transport.
    Authors: Tu H, Kastin AJ, Hsuchou H, Pan W
    J. Cell. Physiol., 2008-02-01;214(2):301-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  34. High circulating leptin receptors with normal leptin sensitivity in liver-specific insulin receptor knock-out (LIRKO) mice.
    Authors: Cohen SE, Kokkotou E, Biddinger SB, Kondo T, Gebhardt R, Kratzsch J, Mantzoros CS, Kahn CR
    J. Biol. Chem., 2007-06-07;282(32):23672-8.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  35. Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice
    Authors: Lyvianne Decourtye-Espiard, Maud Clemessy, Patricia Leneuve, Erik Mire, Tatiana Ledent, Yves Le Bouc et al.
    Nutrients
  36. Hypertrophic chondrocytes serve as a reservoir for marrow-associated skeletal stem and progenitor cells, osteoblasts, and adipocytes during skeletal development
    Authors: Jason T Long, Abigail Leinroth, Yihan Liao, Yinshi Ren, Anthony J Mirando, Tuyet Nguyen et al.
    eLife
  37. Effects of Enriched Environment on COX-2, Leptin and Eicosanoids in a Mouse Model of Breast Cancer
    Authors: Rachida Nachat-Kappes, Alexandre Pinel, Kristell Combe, Bruno Lamas, Marie-Chantal Farges, Adrien Rossary et al.
    PLoS ONE
  38. Role of astrocytic leptin receptor subtypes on leptin permeation across hCMEC/D3 human brain endothelial cells
    Authors: Hung Hsuchou, Abba J. Kastin, Hong Tu, N. Joan Joan Abbott, Pierre-Olivier Couraud, Weihong Pan
    Journal of Neurochemistry
  39. Single nuclei RNA-seq of mouse placental labyrinth development
    Authors: Bryan Marsh, Robert Blelloch
    eLife
  40. Loss of Adenylyl Cyclase 6 in Leptin Receptor‐Expressing Stromal Cells Attenuates Loading‐Induced Endosteal Bone Formation
    Authors: Mathieu Riffault, Gillian P Johnson, Madeline M Owen, Behzad Javaheri, Andrew A Pitsillides, David A Hoey
    JBMR Plus
  41. Combined effects of obesity and di-(2-ethylhexyl) phthalate on testosterone levels and kisspeptin/GPR54 expression in hypothalamus and testes of male mice
    Authors: Ling-Ling Zhai, Jian Zhao, Ying-Long Bai, Wei Wei, Qi Sun, Li-Hong Jia
    Journal of the Chinese Medical Association
  42. Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageing
    Authors: Carl A. Mitchell, Evgenia V. Verovskaya, Fernando J. Calero-Nieto, Oakley C. Olson, James W. Swann, Xiaonan Wang et al.
    Nature Cell Biology
  43. Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass
    Authors: Bo Shen, Kristy Vardy, Payton Hughes, Alpaslan Tasdogan, Zhiyu Zhao, Rui Yue et al.
    eLife
  44. JAK/STAT3-Regulated Fatty Acid beta -Oxidation Is Critical for Breast Cancer Stem Cell Self-Renewal and Chemoresistance
    Authors: Tianyi Wang, Johannes Francois Fahrmann, Heehyoung Lee, Yi-Jia Li, Satyendra C. Tripathi, Chanyu Yue et al.
    Cell Metabolism

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Mouse Leptin R Antibody
By Anonymous on 08/31/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: bone marrow Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse Leptin R Antibody AF497
Mouse Leptin R Antibody
By Michele Doucet on 04/21/2016
Application: Flow Sample Tested: Bone marrow cells Species: Goat

0.2ug/ml/10^6 cells