Home / Kirrel2/NEPH3 / Mouse Kirrel2/NEPH3 Antibody

Mouse Kirrel2/NEPH3 Antibody

Catalog #: AF2930
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2930
AF2930-SP
Product Details
Citations (7)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Kirrel2/NEPH3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Kirrel2/NEPH3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human Kirrel2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kirrel2/NEPH3
Ser19-Asp501
Accession # Q7TSU7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Kirrel2 (Catalog # 2930-K2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kirrel2/NEPH3

Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107 kDa type I transmembrane adhesion protein belonging to the immunoglobulin superfamily (1, 2). Kirrel2 is one of several proteins expressed both in pancreatic islet beta cells and kidney glomerulus, and is also found in brain and lymph nodes (1‑9). Mouse Kirrel2 cDNA encodes a 700 amino acid (aa) protein that contains a 16 aa signal sequence, a 491 aa extracellular domain with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 172 aa intracellular domain with a nephrin-like, conserved podocin binding site (mouse aa 589‑598) (4, 5). An extracellular RGD sequence allows for cell attachment (4). The extracellular domain of mouse Kirrel2 shares 96%, 86%, 85% and 85% aa identity with rat, human, equine and bovine Kirrel2, respectively. It shares 35‑37% aa identity with other mouse Kirrel proteins. One mouse isoform that has been described is missing the transmembrane domain (aa 501‑533), while another encodes only portions of Ig domains 1‑4 (5). Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier (6). Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet beta cell function (7). In the brain, Kirrel2 is present in olfactory neurons; but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant (8). It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord (9).

References
  1. Sun C. et al. (2003) Genomics 82:130.
  2. Ihalmo, P. et al. (2003) Biochem. Biophys. Res. Commun. 300:364.
  3. Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.
  4. Sellin, L. et al. (2003) FASEB J. 17:115.
  5. SwissProt Accession # Q7TSU7.
  6. Ihalmo, P. et al. (2007) Nephrol. Dial. Transplant 22:1903.
  7. Rinta-Valkama, J. et al. (2007) Diabetes Metab. Res. Rev. 23:119.
  8. Serizawa, S. et al. (2006) Cell 127:1057.
  9. Minaki, Y. et al. (2005) Neurosci. Res. 52:250.
Long Name
Kin of Irregular Chiasm-like 2, Splice Variant A
Entrez Gene IDs
84063 (Human); 243911 (Mouse)
Alternate Names
DKFZp564A1164; Filtrin; FILTRINkin of IRRE-like protein 2; kin of IRRE like 2 (Drosophila); Kin of irregular chiasm-like protein 2; Kirrel2; MGC15718; NEPH3; NEPH3Nephrin-like protein 3; nephrin-like 3; nephrin-like gene 1; NLG1; NLG1kin of irregular chiasm-like 2

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Citations for Mouse Kirrel2/NEPH3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2 epsilon in vomeronasal sensory neurons
    Authors: Jennifer M Lin, Tyler A Mitchell, Megan Rothstein, Alison Pehl, Ed Zandro M Taroc, Raghu R Katreddi et al.
    eLife
  2. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster
    Authors: Miguel-Escalada I, Maestro MA, Balboa D et al.
    Developmental cell
  3. beta3GnT2 null mice exhibit defective accessory olfactory bulb innervation.
    Authors: Henion T, Madany P, Faden A, Schwarting G
    Mol Cell Neurosci, 2012-09-21;52(0):73-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. beta3GnT2 maintains adenylyl cyclase-3 signaling and axon guidance molecule expression in the olfactory epithelium.
    Authors: Henion TR, Faden AA, Knott TK, Schwarting GA
    J. Neurosci., 2011-04-27;31(17):6576-86.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  5. Genome-Wide Screen Reveals Rhythmic Regulation of Genes Involved in Odor Processing in the Olfactory Epithelium
    Authors: Manjana Saleh, Karsten Jürchott, Sonja Oberland, Eva M. Neuhaus, Achim Kramer, Ute Abraham
    Journal of Biological Rhythms
  6. Distorted Coarse Axon Targeting and Reduced Dendrite Connectivity Underlie Dysosmia after Olfactory Axon Injury
    Authors: Aya Murai, Ryo Iwata, Satoshi Fujimoto, Shuhei Aihara, Akio Tsuboi, Yuko Muroyama et al.
    eNeuro
  7. Kirrel2 is differentially required in populations of olfactory sensory neurons for the targeting of axons in the olfactory bulb
    Authors: Neelima Vaddadi, Katrine Iversen, Reesha Raja, Alina Phen, Alexandra Brignall, Emilie Dumontier et al.
    Development

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