Mouse Integrin beta 1/CD29 Antibody Summary
Gln21-Asp728
Accession # P09055
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Integrin beta 1/CD29 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
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Integrin beta 1/CD29 in L‑929 Mouse Cell Line. Integrin beta 1/CD29 was detected in immersion fixed L-929 mouse fibroblast cell line using Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.
Product Datasheets
Citations for Mouse Integrin beta 1/CD29 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Cyclooxygenase-2 induced ?1-integrin expression in NSCLC and promoted cell invasion via the EP1/MAPK/E2F-1/FoxC2 signal pathway
Sci Rep, 2016-09-22;6(0):33823.
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LGL1 binds to Integrin beta1 and inhibits downstream signaling to promote epithelial branching in the mammary gland
Authors: R Ma, D Gong, H You, C Xu, Y Lu, G Bergers, Z Werb, OD Klein, CK Petritsch, P Lu
Cell Reports, 2022-02-15;38(7):110375.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection
Authors: M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi
PloS Pathogens, 2021-12-15;17(12):e1010083.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Higher Sensitivity of GABAergic Compared to Glutamatergic Neurons to Growth-Promoting C3bot Treatment Is Mediated by Vimentin
Authors: A Adolf, P Turko, A Rohrbeck, I Just, I Vida, G Ahnert-Hil, M Höltje
Front Cell Neurosci, 2020-11-03;14(0):596072.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo
Authors: M Reed, AC Luissint, V Azcutia, S Fan, MN O'Leary, M Quiros, J Brazil, A Nusrat, CA Parkos
Nat Commun, 2019-11-01;10(1):5004.
Species: Mouse
Sample Types: Cells
Applications: IF -
Bush-like integrin filament networks associated with hyaloid vasculature in murine neonate eyes
Authors: T Iwanaga, J Nio-Kobaya, H Takahashi-
Biomed. Res., 2019-01-01;40(2):79-85.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Autologous transplantation of adipose-derived stem cells improves functional recovery of skeletal muscle without direct participation in new myofiber formation
Authors: A Gorecka, S Salemi, D Haralampie, F Moalli, D Stroka, D Candinas, D Eberli, L Brügger
Stem Cell Res Ther, 2018-07-17;9(1):195.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy
Authors: A Kishimoto, S Kimura, J Nio-Kobaya, H Takahashi-, AM Park, T Iwanaga
Exp. Eye Res., 2018-03-27;172(0):1-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Antibody-based targeting of alternatively spliced tissue factor: a new approach to impede the primary growth and spread of pancreatic ductal adenocarcinoma
Oncotarget, 2016-05-03;7(18):25264-75.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: IHC-P, Neutralization -
Crawling Phagocytes Recruited in the Brain Vasculature after Pertussis Toxin Exposure through IL6, ICAM1 and ITGalphaM.
Authors: Richard JF, Roy M, Audoy-Remus J, Tremblay P, Vallieres L
Brain Pathol., 2011-05-23;21(0):661.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Absence of keratin 8 confers a paradoxical microflora-dependent resistance to apoptosis in the colon.
Authors: Habtezion A, Toivola DM, Asghar MN, Kronmal GS, Brooks JD, Butcher EC, Omary MB
Proc. Natl. Acad. Sci. U.S.A., 2011-01-10;108(4):1445-50.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot
FAQs
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Average Rating: 2.3 (Based on 3 Reviews)
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Not-so-negative control IF staining of rat anti-mouse integrin beta 1/CD29 at 15ug/mL on FFPE human tissue section, incubated at 4C overnight, and followed by Invitrogen Goat anti-Rat Alexa 488 (green) for 1 hour. Counterstained for mitochondria (red) and DAPI (blue).
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
UPDATE: This customer needed an anti-mouse specific antibody that could be used in an immunohistochemistry (IHC) application as a negative control to differentiate transplanted human cells from surrounding mouse tissue. The MAB2405 datasheet makes no statement regarding potential cross-reactivity between human and mouse Integrin beta 1 when analyzing natural samples by IHC. Cross-reactivity has only been examined using a recombinant human Integrin beta 1 protein in Western Blotting. Because the anti-mouse Integrin beta 1 antibody displayed some putative cross-reactivity with human Integrin beta 1 protein in IHC samples, other suitable antibodies were discussed and offered as substitutes. None met the customer’s dual needs of specific localized expression and zero guaranteed cross-reactivity with the human version of the protein in natural samples studied by the IHC application.
Integrin B1 antibody was used at the highest concentration (25 µg/ml) with O/N incubation at 4 degrees Celsius, using a secondary antibody chicken anti-rat Alexa 594 (Thermofisher). Dapi was used to stain the cell nuclei. A TUBB3 antibody with green labeling was used to visualize enteric neurons. Signal from Integrin B1 could not be detected where it was expected.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
