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Mouse IL-15R alpha Biotinylated Antibody

Catalog #: BAF551
24 Citations Datasheet / COA / SDS
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BAF551
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Citations (24)
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Mouse IL-15R alpha Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-15 R alpha in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) IL‑15 R alpha is observed and less than 1% cross-reactivity with rhIL-2 R alpha, recombinant mouse (rm) IL-2 R beta, and rmIL-2 R gamma is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑15 R alpha Fc Chimera (Catalog # 551-MR)
Flow Cytometry
2.5 µg/106 cells
EL‑4 mouse lymphoblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-15R alpha

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL‑15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF‑ alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References
  1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
  3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
  4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
Long Name
Interleukin 15 Receptor alpha
Entrez Gene IDs
3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)
Alternate Names
CD215 antigen; CD215; IL15 R alpha; IL-15 R alpha; IL-15 receptor subunit alpha; IL-15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179

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Citations for Mouse IL-15R alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Qualitatively Different Memory CD8+ T Cells Are Generated after Lymphocytic Choriomeningitis Virus and Influenza Virus Infections
    Authors: Scott N. Mueller, William A. Langley, Guimei Li, Adolfo García-Sastre, Richard J. Webby, Rafi Ahmed
    The Journal of Immunology
  2. Murine Th17 cells utilize IL-2 receptor gamma chain cytokines but are resistant to cytokine withdrawal-induced apoptosis
    Authors: Daniel J. Neitzke, Jacob S. Bowers, Kristina Andrijauskaite, Nathaniel S. O’Connell, Elizabeth Garrett-Mayer, John M. Wrangle et al.
    Cancer Immunology, Immunotherapy
  3. IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation.
    Authors: Mortier E, Woo T, Advincula R, Gozalo S, Ma A
    J. Exp. Med., 2008-05-05;205(5):1213-25.
  4. Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells.
    Authors: Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B
    Proc. Natl. Acad. Sci. U.S.A., 2010-05-10;107(21):9759-64.
  5. NK cells mediate clearance of CD8+ T cell-resistant tumors in response to STING agonists
    Authors: Christopher J. Nicolai, Natalie Wolf, I-Chang Chang, Georgia Kirn, Assaf Marcus, Chudi O. Ndubaku et al.
    Science Immunology
  6. IL-18, but not IL-15, contributes to the IL-12-dependent induction of NK-cell effector functions by Leishmania infantum in vivo
    Authors: Simone Haeberlein, Heidi Sebald, Christian Bogdan, Ulrike Schleicher
    European Journal of Immunology
  7. NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity
    Authors: N Simonovi?, A Witalisz-S, K Meissl, C Lassnig, U Reichart, T Kolbe, M Farlik, C Bock, V Sexl, M Müller, B Strobl
    J. Immunol., 2019-02-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. IL-15 signaling promotes adoptive effector T-cell survival and memory formation in irradiation-induced lymphopenia
    Cell Biosci, 2016-05-06;6(0):30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. IL-15 receptor alpha signaling constrains the development of IL-17-producing gammadelta T cells.
    Authors: Colpitts S, Puddington L, Lefrancois L
    Proc Natl Acad Sci U S A, 2015-07-20;112(31):9692-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Different NK cell developmental events require different levels of IL-15 trans-presentation.
    Authors: Lee GA, Liou YH, Wang SW, Ko KL, Jiang ST, Liao NS
    J. Immunol., 2011-06-29;187(3):1212-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha.
    Authors: Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y
    Blood, 2011-02-08;117(15):4032-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
    Authors: Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW
    J. Immunol., 2009-06-15;182(12):7398-407.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Interleukin-15 combined with an anti-CD40 antibody provides enhanced therapeutic efficacy for murine models of colon cancer.
    Authors: Zhang M, Yao Z, Dubois S, Ju W, Muller JR, Waldmann TA
    Proc. Natl. Acad. Sci. U.S.A., 2009-04-21;106(18):7513-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans.
    Authors: Rowley J, Monie A, Hung CF, Wu TC
    J. Immunol., 2008-12-15;181(12):8237-47.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development.
    Authors: Vang KB, Yang J, Mahmud SA, Burchill MA, Vegoe AL, Farrar MA
    J. Immunol., 2008-09-01;181(5):3285-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.
    Authors: Rubinstein MP, Lind NA, Purton JF, Filippou P, Best JA, McGhee PA, Surh CD, Goldrath AW
    Blood, 2008-08-08;112(9):3704-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. A synaptic basis for paracrine interleukin-2 signaling during homotypic T cell interaction.
    Authors: Sabatos CA, Doh J, Chakravarti S, Friedman RS, Pandurangi PG, Tooley AJ, Krummel MF
    Immunity, 2008-07-31;29(2):238-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. Regulatory T cells control dendritic cell/NK cell cross-talk in lymph nodes at the steady state by inhibiting CD4+ self-reactive T cells.
    Authors: Terme M, Chaput N, Combadiere B, Ma A, Ohteki T, Zitvogel L
    J. Immunol., 2008-04-01;180(7):4679-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. IL-15 does not affect IEL development in the thymus but regulates homeostasis of putative precursors and mature CD8 alpha alpha+ IELs in the intestine.
    Authors: Lai YG, Hou MS, Hsu YW, Chang CL, Liou YH, Tsai MH, Lee F, Liao NS
    J. Immunol., 2008-03-15;180(6):3757-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Antigen controls IL-7Ralpha expression levels on CD8 T cells during full activation or tolerance induction.
    Authors: Hammerbeck CD, Mescher MF
    J. Immunol., 2008-02-15;180(4):2107-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  21. DAP12 signaling directly augments proproliferative cytokine stimulation of NK cells during viral infections.
    Authors: French AR, Sjolin H, Kim S, Koka R, Yang L, Young DA, Cerboni C, Tomasello E, Ma A, Vivier E, Karre K, Yokoyama WM
    J. Immunol., 2006-10-15;177(8):4981-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
    Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
    J. Immunol., 2004-02-01;172(3):1754-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Autophagy is a critical regulator of memory CD8(+) T cell formation
    Authors: Daniel J Puleston, Hanlin Zhang, Timothy J Powell, Elina Lipina, Stuart Sims, Isabel Panse et al.
    eLife
  24. 1Protein Energy Malnutrition Impairs Homeostatic Proliferation of Memory CD8 T cells
    Authors: Smita S. Iyer, Janel Hart Chatraw, Wendy G. Tan, E. John Wherry, Todd C. Becker, Rafi Ahmed et al.
    The Journal of Immunology

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