Mouse IL-13 R alpha 2 Antibody Summary
Leu22-Lys334
Accession # O88786
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑13 R alpha 2 in Mouse Splenocytes. IL-13 Ra2 was detected in immersion fixed mouse splenocytes using Mouse IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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IL‑13 R alpha 2 in Mouse Thymus. IL-13 Ra2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13 R alpha 2
Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
The mouse IL-13 R alpha 2 cDNA encodes a 383 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 313 residue extracellular domain, a 22 aa residue transmembrane region, and a 27 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the cytoplasmic domain of IL-13 R alpha 2 lacks the box 1 and box 2 signaling motif and is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with IL-4 R alpha to form a high-affinity receptor complex capable of transducing both an IL-4-dependent and an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 amino acid residues of mouse IL-13 R alpha 2 are identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine. Recombinant mouse IL-13 R alpha /Fc chimera has been shown to bind IL-13 with high affinity and is a potent IL-13 antagonist.
- Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
- Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
Product Datasheets
Citations for Mouse IL-13 R alpha 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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IL-13R?2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses
Nat Commun, 2016-09-15;7(0):12752.
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Innate immune regulates cutaneous sensory IL-13 receptor alpha 2 to promote atopic dermatitis
Authors: S Xiao, Z Lu, M Steinhoff, Y Li, T Buhl, M Fischer, W Chen, W Cheng, R Zhu, X Yan, H Yang, Y Liu, Y Dou, W Wang, J Wang, J Meng
Brain, Behavior, and Immunity, 2021-08-13;98(0):28-39.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice
Authors: RK Radhakrish, RS Thandi, D Tripathi, P Paidipally, MK McAllister, S Mulik, B Samten, R Vankayalap
JCI Insight, 2020-03-12;5(5):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Interleukin‐13 and its signaling pathway is associated with obesity‐related colorectal tumorigenesis
Authors: Shimpei Matsui, Koji Okabayashi, Masashi Tsuruta, Kohei Shigeta, Ryo Seishima, Takashi Ishida et al.
Cancer Science
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Unexpected involvement of IL-13 signalling via a STAT6 independent mechanism during murine IgG2a development following viral vaccination
Authors: MA Hamid, RJ Jackson, S Roy, M Khanna, C Ranasinghe
Eur. J. Immunol., 2018-05-10;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice
Authors: T Sezaki, Y Hirata, T Hagiwara, YI Kawamura, T Okamura, R Takanashi, K Nakano, M Tamura-Nak, LC Burkly, T Dohi
World J. Gastroenterol., 2017-04-07;23(13):2294-2307.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, Neutralization -
GdCl3 Attenuates Schistosomiasis japonicum Egg-Induced Granulomatosis Accompanied by Decreased Macrophage Infiltration in Murine Liver.
Authors: Zheng S, Lu Q, Xu Y, Wang X, Shen J, Wang W
PLoS ONE, 2015-08-28;10(8):e0132222.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
J. Allergy Clin. Immunol., 2008-08-09;122(3):625-32.
Species: Mouse
Sample Types: Cell Culture Supernates, Cell Lysates, Whole Cells
Applications: ELISA Development, Flow Cytometry, Western Blot -
Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia.
Authors: Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y
Am. J. Physiol. Lung Cell Mol. Physiol., 2006-06-16;291(5):L862-70.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
J. Immunol., 2006-06-15;176(12):7495-501.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Impact of interleukin-13 responsiveness on the synthetic and proliferative properties of Th1- and Th2-type pulmonary granuloma fibroblasts.
Authors: Jakubzick C, Choi ES, Kunkel SL, Joshi BH, Puri RK, Hogaboam CM
Am. J. Pathol., 2003-05-01;162(5):1475-86.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
IL-13 immunotoxin accelerates resolution of lung pathological changes triggered by silica particles in mice.
Authors: Ferreira T, de Arantes A, do Nascimento C, Olsen P, Trentin P, Rocco P, Hogaboam C, Puri R, Martins M, Silva P
J Immunol, 2013-10-16;191(10):5220-9.
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