Mouse IL-12 Antibody

Name: Mouse IL-12 Antibody
Brand: R&D Systems
SKU: AF-419-NA
Rating: 5 (3 reviews)

Catalog #: AF-419-NA
15 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-419-NA
AF-419-SP

Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12 Antibody.

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All IL-12 Antibodies

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Citations (15)

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Supplemental Products

Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-12 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-12 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing and reducing conditions), this antibody shows less than 10% cross-reactivity with recombinant human IL-12.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12
Mouse IL-12 heterodimer: Mouse IL-12 p40 (Met23-Ser335) Accession # P43432, Mouse IL-12 p35 (Arg23-Ala215) Accession # NP_032377

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑12 (Catalog # 419-ML)

Neutralization

Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The Neutralization Dose (ND₅₀) is typically 0.01-0.06 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12 Antibody. Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND₅₀ is typically 0.01-0.06 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 - G158 peptide and the p35 E159‑A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

Long Name

Interleukin 12

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Alternate Names

CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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The inhibition of malignant melanoma cell invasion of bone by the TLR7 agonist R848 is dependent upon pro-inflammatory cytokines produced by bone marrow macrophages
Authors: Y Manome, D Suzuki, A Mochizuki, E Saito, K Sasa, K Yoshimura, T Inoue, M Takami, K Inagaki, T Funatsu, R Kamijo
Oncotarget, 2018-07-06;9(52):29934-29943.
Exosomes Derived From Septic Mouse Serum Modulate Immune Responses via Exosome-Associated Cytokines
Authors: Kun Gao, Jingmiao Jin, Chenyang Huang, Jianhang Li, Haihua Luo, Lei Li et al.
Frontiers in Immunology
The p40 subunit of interleukin (IL)-12 promotes stabilization and export of the p35 subunit: implications for improved IL-12 cytokine production
Authors: Rashmi Jalah, Margherita Rosati, Brunda Ganneru, Guy R. Pilkington, Antonio Valentin, Viraj Kulkarni et al.
Journal of Biological Chemistry
Distinct ROR?t-dependent Th17 immune responses are required for autoimmune pathogenesis and protection against bacterial infection
Authors: Zhong, X;Wu, H;Zhang, W;Shi, Y;Gwack, Y;Xue, HH;Sun, Z;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Acquired Immunostimulatory Phenotype of Migratory CD103+ Dendritic Cells Promotes Alloimmunity Following Corneal Transplantation
Authors: Blanco, T;Nakagawa, H;Musayeva, A;Krauthammer, M;Singh, RB;Narimatsu, A;Ge, H;Shoushtari, SI;Dana, R;
JCI insight
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Crucial role of OX40/OX40L signaling in a murine model of asthma
Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
Mol Med Rep, 2018-01-18;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay
Efficient expression of bioactive murine IL12 as a self-processing P2A polypeptide driven by inflammation-regulated promoters in tumor cell lines.
Authors: Lorenzo C, Perez-Chacon G, Garaulet G, Mallorquin Z, Zapata J, Rodriguez A
Cancer Gene Ther, 2015-10-09;22(11):542-51.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Viral antigen mediated NKp46 activation of NK cells results in tumor rejection via NK-DC crosstalk.
Authors: Chinnery, Fay, King, Catherin, Elliott, Tim, Bateman, Andrew R, James, Edward
Oncoimmunology, 2012-09-01;1(6):874-883.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
Authors: Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G
J. Immunol., 2010-12-13;186(2):869-77.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Expression of bioactive single-chain murine IL-12 in transgenic plants.
Authors: Liu J, Dolan MC, Reidy M, Cramer CL
J. Interferon Cytokine Res., 2008-06-01;28(0):381-92.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells.
Authors: Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F
Immunol. Lett., 2007-12-17;116(1):86-94.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo.
Authors: Hajishengallis G, Shakhatreh MA, Wang M, Liang S
J. Immunol., 2007-08-15;179(4):2359-67.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Targeting Xcr1 on Dendritic Cells Rapidly Induce Th1-Associated Immune Responses That Contribute to Protection Against Influenza Infection
Authors: Demo Yemane Tesfaye, Sonja Bobic, Anna Lysén, Peter Csaba Huszthy, Arnar Gudjonsson, Ranveig Braathen et al.
Frontiers in Immunology

TLR Stimulation during T-cell Activation Lowers PD-1 Expression on CD8+ T Cells
Authors: Christopher D. Zahm, Viswa T. Colluru, Sean J. McIlwain, Irene M. Ong, Douglas G. McNeel
Cancer Immunology Research

Activating a collaborative innate-adaptive immune response to control metastasis
Authors: Lijuan Sun, Tim Kees, Ana Santos Almeida, Bodu Liu, Xue-Yan He, David Ng et al.
Cancer Cell