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Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

Catalog #: MHSLB00
6 Citations 1 Review Datasheet / COA / SDS
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MHSLB00
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Mouse IL-1 beta /IL-1F2 Standard Curve
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.312 pg/mL
Assay Range
0.8 - 50 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® HS Mouse IL-1 beta /IL-1F2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse IL-1 beta levels in serum and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant protein. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine® HS kit standards. These results indicate that the Quantikine® HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-1 beta.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 4.64 14.1 28.5 4.89 14.5 28.9
Standard Deviation 0.13 0.378 0.59 0.413 0.796 1.6
CV% 2.8 2.7 2.1 8.4 5.5 5.5

Recovery

The recovery of mouse IL-1 beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 98 91-105
Heparin Plasma (n=4) 92 84-99
Serum (n=4) 95 87-101

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-1 beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse IL-1 beta /IL-1F2 Linearity

Scientific Data

N/A Mouse IL-1 beta /IL-1F2 Standard Curve View Larger

Mouse IL-1 beta /IL-1F2 Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

The Interleukin 1 (IL-1) family of proteins consists of IL-1 alpha, IL-1 beta, and the IL-1 receptor antagonist (IL-1ra). IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions (1). IL-1 is not produced by unstimulated cells of healthy individuals with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. However, in response to inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is seen. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulindependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases (2-5). 

 IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid (aa) level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into mature proteins of approximately 17.5 kDa (6, 7). Cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response (2, 8). Neither IL-1 alpha nor IL-1 beta contains a typical hydrophobic signal peptide (9-11), but evidence suggests that these factors can be secreted by non-classical pathways (12, 13). A portion of unprocessed IL-1 alpha can be presented on the cell membrane and may retain biological activity (14). The precursor form of IL-1 beta, unlike the IL-1 alpha precursor, shows little or no biological activity in comparison to the processed form (13, 15). Both unprocessed and mature forms of IL-1 beta are exported from the cell. 
 IL-1 alpha and IL-1 beta exert their effects through immunoglobulin superfamily receptors that additionally bind IL-1ra. The 80 kDa transmembrane type I receptor (IL-1 RI) is expressed on T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes (16, 17). The 68 kDa transmembrane type II receptor (IL-1 RII) is expressed on B cells, neutrophils, and bone marrow cells (18). The two IL-1 receptor types show approximately 28% homology in their extracellular domains but differ significantly in that the type II receptor has a cytoplasmic domain of only 29 aa, whereas the type I receptor has a 213 aa cytoplasmic domain. IL-1 RII does not appear to signal in response to IL-1 and may function as a decoy receptor that attenuates IL-1 function (19). The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction (20). IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 (21, 22). Soluble forms of both IL-1 RI and IL-1 RII have been detected in human plasma, synovial fluids, and the conditioned media of several human cell lines (23, 24). In addition, IL-1 binding proteins that resemble soluble IL-1 RII are encoded by vaccinia and cowpox viruses (25).

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Citations for Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. IL-6 Deficiency Attenuates Skeletal Muscle Atrophy by Inhibiting Mitochondrial ROS Production through the Upregulation of PGC-1alpha in Septic Mice
    Authors: B Yang, X Yang, X Sun, J Shi, Y Shen, R Chen
    Oxidative Medicine and Cellular Longevity, 2022-04-27;2022(0):9148246.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Distinct pro-inflammatory properties of myeloid cell-derived apolipoprotein E2 and E4 in atherosclerosis promotion
    Authors: E Igel, A Haller, PR Wolfkiel, M Orr-Asman, A Jaeschke, DY Hui
    The Journal of Biological Chemistry, 2021-08-21;0(0):101106.
    Species: Mouse
    Sample Types: Plasma
  3. Nucleotide-binding domain and leucine-rich-repeat-containing protein X1 deficiency induces nicotinamide adenine dinucleotide decline, mechanistic target of rapamycin activation, and cellular senescence and accelerates aging lung-like changes
    Authors: HJ Shin, SH Kim, HJ Park, MS Shin, I Kang, MJ Kang
    Aging Cell, 2021-06-04;0(0):e13410.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice
    Authors: SY Cheon, BN Koo, SY Kim, EH Kam, J Nam, EJ Kim
    Scientific Reports, 2021-04-16;11(1):8376.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Orthopedic surgery-induced cognitive dysfunction is mediated by CX3CL1/R1 signaling
    Authors: I Cho, JM Kim, EJ Kim, SY Kim, EH Kam, E Cheong, M Suh, BN Koo
    Journal of Neuroinflammation, 2021-04-15;18(1):93.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. CXCL14 Overexpression Attenuates Sepsis-Associated Acute Kidney Injury by Inhibiting Proinflammatory Cytokine Production
    Authors: J Lv, ZL Wu, Z Gan, P Gui, SL Yao
    Mediators Inflamm., 2020-03-31;2020(0):2431705.
    Species: Mouse
    Sample Types: Serum, Tissue Homogenate

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Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Anonymous on 09/14/2019
Sample Tested: Serum and Plasma