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Mouse IgG NorthernLights™ NL557-conjugated Antibody

Catalog #: NL007
30 Citations Datasheet / COA / SDS
NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL007
Detection of Mouse IgG Primary Antibody by Immunocytochemistry.
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Citations (30)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG NorthernLights™ NL557-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Mouse IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Use at a 1:200 dilution
 
Immunohistochemistry
Use at a 1:200 dilution
 
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Mouse IgG Primary Antibody by Immunocytochemistry. Detection of human Vimentin by incubating cells with 8 μg/mL of Mouse anti-Human Vimentin monoclonal antibody (MAB21052) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Mouse IgG NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Generation of a human induced pluripotent stem cell line from a patient with GM3 synthase deficiency using self-replicating RNA vector
    Authors: Tonin, R;Feo, F;Falliano, S;Giunti, L;Calamai, M;Procopio, E;Mari, F;Sciruicchio, V;Conti, V;Fanelli, I;Bambi, F;Guerrini, R;Morrone, A;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Acute high fat diet impairs macrophage supported intestinal damage resolution
    Authors: AA Hill, M Kim, DF Zegarra-Ru, LC Chang, KC Norwood, A Assié, WH Wu, MC Renfroe, HW Song, AM Major, BS Samuel, JM Hyser, RS Longman, GE Diehl
    JCI Insight, 2023-02-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Essential role of the mitochondrial Na+/Ca2+ exchanger NCLX in mediating PDE2-dependent neuronal survival and learning
    Authors: Maya Rozenfeld, Ivana Savic Azoulay, Tsipi Ben Kasus Ben Kasus Nissim, Alexandra Stavsky, Moran Melamed, Grace Stutzmann et al.
    Cell Reports
  4. Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
    Authors: H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride
    Stem Cell Research, 2022-10-31;65(0):102958.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Studies on a widely-recognized snail model species (Lymnaea stagnalis) provide further evidence that vertebrate steroids do not have a hormonal role in the reproduction of mollusks
    Authors: István Fodor, Tamar Schwarz, Bence Kiss, Antal Tapodi, János Schmidt, Alex R. O. Cousins et al.
    Front Endocrinol (Lausanne)
  6. Intracellular aggregation of peptide-reprogrammed small molecule nanoassemblies enhances cancer chemotherapy and combinatorial immunotherapy
    Authors: Jinrong Peng, Yao Xiao, Qian Yang, Qingya Liu, Yu Chen, Kun Shi et al.
    Acta Pharmaceutica Sinica B
  7. Rapid 3D phenotypic analysis of neurons and organoids using data-driven cell segmentation-free machine learning
    Authors: P Mergenthal, S Hariharan, JM Pemberton, C Lourenco, LZ Penn, DW Andrews
    PLoS computational biology, 2021-02-22;17(2):e1008630.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Synergistic induction of apoptosis in resistant head and neck carcinoma and leukemia by alkoxyamide-based histone deacetylase inhibitors
    Authors: LA Alves Avel, C Schrenk, M Sönnichsen, A Hamacher, FK Hansen, J Schliehe-D, A Borkhardt, S Bhatia, MU Kassack, T Kurz
    European Journal of Medicinal Chemistry, 2020-12-17;211(0):113095.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM
    Authors: G Kaufman, D Skrtic
    Int J Mol Sci, 2020-10-03;21(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts
    Authors: Dhananjay V. Deshmukh, Nils Pasquero, Gajraj Rathore, Joel Zvick, Ori Bar‐Nur, Jurg Dual et al.
    Bioengineering & Translational Medicine
  11. The identification of nuclear &alphav&beta3 integrin in ovarian cancer: non-paradigmal localization with cancer promoting actions
    Authors: C Seraya-Bar, A Weisz, E Shinderman, S Teper-Roth, D Stamler, N Arbib, Y Kadan, A Fishman, D Kidron, E Edelstein, M Ellis, O Ashur-Fabi
    Oncogenesis, 2020-07-29;9(7):69.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Antiresorptive activity of osteoprotegerin requires an intact heparan sulfate-binding site
    Authors: M Li, D Xu
    Proc. Natl. Acad. Sci. U.S.A., 2020-07-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis
    Authors: X Hou, G Chen, W Bracamonte, HS Choi, NL Diny, J Sung, D Hughes, T Won, MK Wood, MV Talor, DJ Hackam, K Klingel, G Davogustto, H Taegtmeyer, I Coppens, JG Barin, D ?iháková
    Cell Rep, 2019-07-02;28(1):172-189.e7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Telomerase reverse transcriptase ameliorates lung fibrosis by protecting alveolar epithelial cells against senescence
    Authors: T Liu, F Gonzalez D, Y Zhao, Z Wu, AE Rinke, KK Kim, SH Phan
    J. Biol. Chem., 2019-04-18;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Reduced NLRP3 Gene Expression Limits the IL-1? Cleavage via Inflammasome in Monocytes from Severely Injured Trauma Patients
    Authors: S Kany, JP Horstmann, R Sturm, K Mörs, B Relja
    Mediators Inflamm., 2018-05-09;2018(0):1752836.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. A biomaterials approach to influence stem cell fate in injectable cell-based therapies
    Authors: MH Amer, FRAJ Rose, KM Shakesheff, LJ White
    Stem Cell Res Ther, 2018-02-21;9(1):39.
  17. Calcium phosphate particles stimulate interleukin-1 beta release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release
    Authors: Yana Dautova, Alexander N. Kapustin, Kevin Pappert, Matthias Epple, Hanneke Okkenhaug, Simon J. Cook et al.
    Journal of Molecular and Cellular Cardiology
  18. Free paclitaxel-loaded E-selectin binding peptide modified micelle self-assembled from hyaluronic acid-paclitaxel conjugate inhibit breast cancer metastasis in a murine model
    Authors: X Han, X Dong, J Li, M Wang, L Luo, Z Li, X Lu, R He, R Xu, M Gong
    Int J Pharm, 2017-05-31;528(2):33-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Antithrombin III improved neutrophil extracellular traps in lung after the onset�of�endotoxemia
    Authors: Michiko Ishikawa
    J. Surg. Res, 2016-09-28;208(0):140-150.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Spatial Geometries of Self-Assembled Chitohexaose Monolayers Regulate Myoblast Fusion
    Authors: Pornthida Poosala, Hirofumi Ichinose, Takuya Kitaoka
    International Journal of Molecular Sciences
  21. Phenotypic plasticity in normal breast derived epithelial cells.
    Authors: Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S
    BMC Cell Biol, 2014-06-10;15(0):20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Atorvastatin Treatment of Rats with Ischemia-Reperfusion Injury Improves Adipose-Derived Mesenchymal Stem Cell Migration and Survival via the SDF-1 alpha /CXCR-4 Axis
    Authors: Anping Cai, Ruofeng Qiu, Liwen Li, Dongdan Zheng, Yugang Dong, Danqing Yu et al.
    PLoS ONE
  23. Primitive stem cells derived from bone marrow express glial and neuronal markers and support revascularization in injured retina exposed to ischemic and mechanical damage.
    Stem Cells Dev., 2011-10-24;21(9):1488-500.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells.
    Authors: Gopalakrishna-Pillai S, Iverson LE
    PLoS ONE, 2011-06-15;6(6):e20663.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Functional characterization of a novel mutation localized in the start codon of the tissue-nonspecific alkaline phosphatase gene.
    Authors: Mentrup B, Marschall C, Barvencik F, Amling M, Plendl H, Jakob F, Beck C
    Bone, 2011-03-16;48(6):1401-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Tumor necrosis factor receptor expression and signaling in renal cell carcinoma.
    Authors: Al-Lamki RS, Sadler TJ, Wang J
    Am. J. Pathol., 2010-06-21;177(2):943-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. NorthernLights in slide-based cytometry and microscopy.
    Authors: Wessels JT, Busse AC, Mahrt J, Hoffschulte B, Mueller GA, Tarnok A, Mittag A
    Cytometry A, 2010-05-01;77(5):420-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  28. Developmental corneal innervation: interactions between nerves and specialized apical corneal epithelial cells.
    Authors: Kubilus JK, Linsenmayer TF
    Invest. Ophthalmol. Vis. Sci., 2009-09-09;51(2):782-9.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  29. Role of clathrin-mediated endocytosis of surfactant protein A by alveolar macrophages in intracellular signaling.
    Authors: Moulakakis C, Stamme C
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-01-09;296(3):L430-41.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  30. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.
    Authors: Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P
    J. Immunol., 2008-08-15;181(4):2772-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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