Mouse IFN-alpha / beta R2 APC-conjugated Antibody

Catalog #: FAB1083A
2 Citations Datasheet / COA / SDS

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FAB1083A

Detection of IFN‑ alpha / beta R2 in A20 Mouse Cell Line by Flow Cytometry.

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Mouse IFN-alpha / beta R2 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFN‑ alpha / beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta, rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R2
Ser22-Ala239 (Lys160Asn)
Accession # Q9D1R7

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IFN‑ alpha / beta R2 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IFN-a/ beta R2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1083A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IFN-alpha/beta R2

IFN-alpha / beta R2 belongs to the type II cytokine receptor family. It complexes with IFN-alpha / beta R1 to form the signaling receptor complex for the family of alpha and beta IFN subtypes. By alternative splicing, IFN-alpha / beta R2 can exist as a secreted soluble protein or as a type I membrane protein.

Long Name

Interferon alpha/beta Receptor 2

Entrez Gene IDs

3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)

Alternate Names

beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta R2; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2

Product Datasheets

Product Datasheet

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Citations for Mouse IFN-alpha / beta R2 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Type I interferon signaling induces melanoma cell-intrinsic PD-1 and its inhibition antagonizes immune checkpoint blockade
Authors: Holzgruber, J;Martins, C;Kulcsar, Z;Duplaine, A;Rasbach, E;Migayron, L;Singh, P;Statham, E;Landsberg, J;Boniface, K;Seneschal, J;Hoetzenecker, W;Berdan, EL;Ho Sui, S;Ramsey, MR;Barthel, SR;Schatton, T;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
In vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target
Authors: Robert T. Manguso, Hans W. Pope, Margaret D. Zimmer, Flavian D. Brown, Kathleen B. Yates, Brian C. Miller et al.
Nature