Mouse IFN-alpha All Subtype Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 82.2 | 590 | 199 | 79.3 | 602 | 195 |
Standard Deviation | 3.04 | 15.9 | 3.08 | 5.11 | 57.4 | 7.93 |
CV% | 3.7 | 2.7 | 1.5 | 6.4 | 9.5 | 4.1 |
Recovery
The recovery of mouse IFN-alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 98 | 91-106 |
EDTA Plasma (n=4) | 105 | 91-121 |
Heparin Plasma (n=4) | 106 | 96-120 |
Serum (n=4) | 102 | 86-118 |
Linearity
Scientific Data
Mouse IFN-alpha All Subtype Quantikine Natural Linearity Competitor Comparison IFN-alpha samples containing a high concentration in various matrices are diluted with appropriate Calibrator Diluent to produce sample values within the dynamic range of the assay. R&D Systems natural linearity is between 98%-103% compared to 125%-180% for the top competitor.
Mouse IFN-alpha All Subtype Quantikine ELISA Spiked Linearity Competitor Comparison IFN-alpha is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. R&D Systems linearity is between 88%-92% compared to 132%-201% for the top competitor.
Mouse IFN-alpha All Subtype Quantikine ELISA Spiked Recovery IFN-alpha is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. R&D Systems recovery is 99%.
Product Datasheets
Preparation and Storage
Background: IFN-alpha
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Citation for Mouse IFN-alpha All Subtype Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Yeast ?-glucan promotes antiviral type I interferon response via dectin-1
Authors: Wang, J;Jin, X;Yan, S;Zhao, H;Pang, D;Ouyang, H;Tang, X;
Veterinary microbiology
Species: Mouse
Sample Types: Serum, Cell Culture Supernates
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