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Mouse GDF-15 DuoSet ELISA

Catalog #: DY6385
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY6385
DY6385-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse GDF-15 ELISA Standard Curve
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse GDF-15 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse GDF-15. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Mouse GDF-15 ELISA Standard Curve View Larger

Mouse GDF-15 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-15

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

Long Name:
Growth Differentiation Factor 15
Entrez Gene IDs:
9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names:
GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

Citations for Mouse GDF-15 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Adipose tissue macrophage infiltration and hepatocyte stress increase GDF-15 throughout development of obesity to MASH
    Authors: L'homme, L;Sermikli, BP;Haas, JT;Fleury, S;Quemener, S;Guinot, V;Barreby, E;Esser, N;Caiazzo, R;Verkindt, H;Legendre, B;Raverdy, V;Cheval, L;Paquot, N;Piette, J;Legrand-Poels, S;Aouadi, M;Pattou, F;Staels, B;Dombrowicz, D;
    Nature communications
    Species: Mouse
    Sample Types: Cell Culture Supernates, Plasma
  2. The gastrointestinal tract is a major source of the acute metformin-stimulated rise in GDF15
    Authors: Kincaid, JWR;Rimmington, D;Tadross, JA;Cimino, I;Zvetkova, I;Kaser, A;Richards, P;Patel, S;O'Rahilly, S;Coll, AP;
    Scientific reports
    Species: Mouse
    Sample Types: Serum
  3. Growth differentiation factor-15 is an IFN-? regulated mediator of infection-induced weight loss and the hepatic FGF21 response
    Authors: Reyes, J;Zhao, Y;Pandya, K;Yap, GS;
    Brain, behavior, and immunity
    Species: Mouse
    Sample Types: Serum
  4. Lac-Phe mediates the anti-obesity effect of metformin
    Authors: Xiao, S;Li, VL;Lyu, X;Chen, X;Wei, W;Abbasi, F;Knowles, JW;Deng, S;Tiwari, G;Shi, X;Zheng, S;Farrell, L;Chen, ZZ;Taylor, KD;Guo, X;Goodarzi, MO;Wood, AC;Chen, YI;Lange, LA;Rich, SS;Rotter, JI;Clish, CB;Tahir, UA;Gerszten, RE;Benson, MD;Long, JZ;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Blood
  5. Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
    Authors: A Hooftman, CG Peace, DG Ryan, EA Day, M Yang, AF McGettrick, M Yin, EN Montano, L Huo, JE Toller-Kaw, V Zecchini, TAJ Ryan, A Bolado-Car, AM Casey, HA Prag, ASH Costa, G De Los San, M Ishimori, DJ Wallace, S Venuturupa, E Nikitopoul, N Frizzell, C Johansson, A Von Kriegs, MP Murphy, C Jefferies, C Frezza, LAJ O'Neill
    Nature, 2023-03-08;615(7952):490-498.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. The GDF15-GFRAL axis mediates chemotherapy-induced fatigue in mice
    Authors: B Chelette, CL Chidomere, R Dantzer
    Brain, Behavior, and Immunity, 2022-11-24;108(0):45-54.
    Species: Mouse
    Sample Types: Plasma
  7. Combined genetic deletion of GDF15 and FGF21 has modest effects on body weight, hepatic steatosis and insulin resistance in high fat fed mice
    Authors: S Patel, A Haider, A Alvarez-Gu, G Bidault, JS El-Sayed M, E Guiu-Jurad, JA Tadross, J Warner, J Harrison, S Virtue, F Scurria, I Zvetkova, M Blüher, KS Small, S O'Rahilly, DB Savage
    Molecular Metabolism, 2022-09-02;65(0):101589.
    Species: Mouse
    Sample Types: Plasma
  8. Aldehyde-driven transcriptional stress triggers an anorexic DNA damage response
    Authors: L Mulderrig, JI Garaycoech, ZK Tuong, CL Millington, FA Dingler, JR Ferdinand, L Gaul, JA Tadross, MJ Arends, S O'Rahilly, GP Crossan, MR Clatworthy, KJ Patel
    Nature, 2021-11-24;600(7887):158-163.
    Species: Mouse
    Sample Types: Serum
  9. Development of insulin resistance preceded major changes in iron homeostasis in mice fed a high-fat diet
    Authors: J Varghese, JV James, R Anand, M Narayanasa, G Rebekah, B Ramakrishn, AJ Nellickal, M Jacob
    J. Nutr. Biochem., 2020-06-02;84(0):108441.
    Species: Mouse
    Sample Types: Serum
  10. Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Authors: KH Kim, SH Kim, DH Han, YS Jo, YH Lee, MS Lee
    Sci Rep, 2018-05-01;8(1):6789.
    Species: Mouse
    Sample Types: Serum

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