Mouse FPRP/PTGFRN Biotinylated Antibody

Catalog #: BAF4495 Datasheet / COA / SDS

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BAF4495

Detection of FPRP/PTGFRN in 3T3‑L1 Mouse Cell Line by Flow Cytometry.

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Mouse FPRP/PTGFRN Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FPRP in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FPRP
Arg22-Pro832
Accession # NP_035327

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse FPRP

Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of FPRP/PTGFRN in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Sheep Anti-Mouse FPRP/PTGFRN Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4495, filled histogram) or isotype control antibody (Catalog # BAF108), followed by Streptavidin-Allophycocyanin (Catalog # F0050).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP/PTGFRN

FPRP (F_{2 alpha} Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F_{2 alpha} receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa 22‑813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa 22‑832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.

Long Name

Prostaglandin F2-alpha Receptor Regulatory Protein

Entrez Gene IDs

5738 (Human); 19221 (Mouse); 29602 (Rat)

Alternate Names

CD315; CD9P-1; FPRP; PTGFRN