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Mouse FGFR2 (IIIb) Antibody

Catalog #: MAB7161
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7161
MAB7161-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FGFR2 (IIIb) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects IIIb isoforms of mouse FGF R2 in direct ELISAs and Western blots. This antibody shows approximately 75% cross-reactivity with recombinant human (rh) FGF R2 (IIIb) isoforms and no cross-reactivity with the IIIc isoform of mouse or human FGF R2 or any isoforms of rhFGF R1, rmFGF R3, or rhFGF R4.
Source
Monoclonal Rat IgG2A Clone # 133730
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF R2 isoforms and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse FGF R2 isoforms
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse FGF R2 alpha (IIIb) Fc Chimera (Catalog # 5496-MF)
Recombinant Mouse FGF R2 beta (IIIb) Fc Chimera (Catalog # 708-MF)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name
Fibroblast Growth Factor Receptor 2
Entrez Gene IDs
2263 (Human); 14183 (Mouse)
Alternate Names
BBDS; BEK; BFR-1; CD332 antigen; CD332; CEK3; CFD1; craniofacial dysostosis 1; EC 2.7.10; EC 2.7.10.1; ECT1; FGF R2; FGFR2; fibroblast growth factor receptor 2; FLJ98662; Jackson-Weiss syndrome; JWS; Keratinocyte growth factor receptorreceptor like 14; KGFR; KSAM; K-sam; soluble FGFR4 variant 4; TK14; TK25

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Citations for Mouse FGFR2 (IIIb) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair
    Authors: Jennifer Quantius
    PLoS Pathog, 2016-06-20;12(6):e1005544.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  2. IFATS collection: Combinatorial peptides identify alpha5beta1 integrin as a receptor for the matricellular protein SPARC on adipose stromal cells.
    Authors: Nie J, Chang B, Traktuev DO, Sun J, March K, Chan L, Sage EH, Pasqualini R, Arap W, Kolonin MG
    Stem Cells, 2008;26(10):2735-45.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. MiR-302/367 regulate neural progenitor proliferation, differentiation timing, and survival in neurulation.
    Authors: Yang S, Yang M, Herrlinger S, Liang C, Lai F, Chen J
    Dev Biol, 2015-10-09;408(1):140-50.

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