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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Catalog #: F0102B
18 Citations 3 Reviews Datasheet / COA / SDS
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F0102B
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Detection of Mouse IgG Primary Antibody by Flow Cytometry
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Citations (18)
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Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors
Source
Polyclonal Goat IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mouse IgG Primary Antibody by Flow Cytometry View Larger

Detection of Mouse IgG Primary Antibody by Flow Cytometry Human peripheral blood granulocytes were stained with Mouse Anti-Human CD157 Monoclonal Antibody (Catalog # MAB47361, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) at 10 µL/106cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Atypical Chemokine Receptor 3 'Senses' CXC Chemokine Receptor 4 Activation Through GPCR Kinase Phosphorylation
    Authors: CT Schafer, Q Chen, JJG Tesmer, TM Handel
    bioRxiv : the preprint server for biology, 2023-03-10;0(0):.
  2. Glucose Enhances Pro-Tumorigenic Functions of Mammary Adipose-Derived Mesenchymal Stromal/Stem Cells on Breast Cancer Cell Lines
    Authors: MR Ambrosio, G Mosca, T Migliaccio, D Liguoro, G Nele, F Schonauer, F D'Andrea, F Liotti, N Prevete, RM Melillo, C Reale, C Ambrosino, C Miele, F Beguinot, V D'Esposito, P Formisano
    Cancers, 2022-11-03;14(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022-09-27;40(13):111423.
    Species: Mouse
    Sample Types: Whole Cells
  4. Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
    Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
    PLoS ONE, 2017-10-10;12(10):e0186317.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells
    Authors: E Tamayo-Orb, L Amo, M Riñón, N Nieto, E Amutio, N Maruri, M Solaun, A Arrieta, S Larrucea
    Oncotarget, 2017-09-27;8(59):99722-99739.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Renal Transplant Patients Biopsied for Cause and Tested for C4d, DSA, and IgG Subclasses and C1q: Which Humoral Markers Improve Diagnosis and Outcomes?
    Authors: JC Cicciarell, NA Lemp, Y Chang, M Koss, K Hacke, N Kasahara, KM Burns, DI Min, R Naraghi, T Shah
    J Immunol Res, 2017-01-15;2017(0):1652931.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  7. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016-11-15;90(0):88-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Secondary Antibody
  8. Interferon-beta inhibits glioma angiogenesis through downregulation of vascular endothelial growth factor and upregulation of interferon inducible protein 10.
    Authors: Takano S, Ishikawa E, Matsuda M, Yamamoto T, Matsumura A
    Int J Oncol, 2014-08-25;45(5):1837-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Crosstalk between mitochondrial ROS and depolarization in the potentiation of TRAIL-induced apoptosis in human tumor cells.
    Authors: Suzuki-Karasaki M, Ochiai T, Suzuki-Karasaki Y
    Int J Oncol, 2013-12-10;44(2):616-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. HER3 targeting of adenovirus by fiber modification increases infection of breast cancer cells in vitro, but not following intratumoral injection in mice.
    Authors: MacLeod S, Elgadi M, Bossi G, Sankar U, Pisio A, Agopsowicz K, Sharon D, Graham F, Hitt M
    Cancer Gene Ther, 2012-10-26;19(12):888-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  11. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
    Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
    J Cell Sci, 2012-07-05;125(0):4475-86.
  12. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
    Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
    PLoS ONE, 2011-02-25;6(2):e16899.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009-07-01;125(1):212-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine.
    Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H
    Biochem. Biophys. Res. Commun., 2008-04-04;370(4):578-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Histone deacetylase inhibitors suppress natural killer cell cytolytic activity.
    Authors: Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J
    FEBS Lett., 2007-03-01;581(7):1317-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Secondary Antibody
  17. Intrinsic Resistance to Cixutumumab Is Conferred by Distinct Isoforms of the Insulin Receptor
    Authors: Amelie Forest, Michael Amatulli, Dale L. Ludwig, Christopher B. Damoci, Ying Wang, Colleen A. Burns et al.
    Molecular Cancer Research
  18. HIV-1 pathogenicity and virion production are dependent on the metabolic phenotype of activated CD4+ T cells
    Authors: Andrea Hegedus, Maia Kavanagh Williamson, Hendrik Huthoff
    Retrovirology

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Reviews for Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Average Rating: 4.7 (Based on 3 Reviews)

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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
By Anonymous on 12/17/2020
Application: Flow Sample Tested: Whole blood Species: Human

Whole blood was diluted with PBS, overlaid atop Ficoll, and centrifuged to separate the PBMC layer. The PBMC layer was washed twice with PBS and cells were aliquoted into centrifuge tubes for staining. Cells were counted and concentration was adjusted to 10,000 cells per uL. 100 uL of cells was aliquoted into each centrifuge tube for staining.

Flow Cytometry Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody F0102B
Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
By Abby Sukarto on 02/13/2020
Application: Flow Sample Tested: Bone Extracts Species: Human
Flow Cytometry Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody F0102B
Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
By Anonymous on 03/24/2017
Application: Flow Sample Tested: Breast cancer cells Species: Human

106 MA-11 cells were incubated with Mouse anti-Human CD44 antibody (red) or Isotype Control IgG (black), followed by staining with 10 uL of PE-conjugated Goat Anti-Mouse secondary antibody (#F0102B).

Flow Cytometry Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody F0102B