Mouse ETV2/ER71 Antibody

Catalog #: MAB7740 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7740
MAB7740-SP

Detection of Mouse ETV2/ER71 by Western Blot.

1 Image

All ETV2/ER71 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ETV2/ER71 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ETV2/ER71 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 827940

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse ETV2/ER71
Asn125-Arg231
Accession # P41163

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse ETV2/ER71 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line and D3 mouse embryonic stem cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse ETV2/ER71 Monoclonal Antibody (Catalog # MAB7740) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for ETV2/ER71 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ETV2/ER71

ETS translocation variant 2 (ETV2), also known as ETSRP and ER71, is a 37 kDa transcription factor that is expressed in presumptive vascular progenitor cells. It plays an important role in vascular development by promoting the expansion of hemangioblasts and vascular endothelial cells while inhibiting differentiation into cardiomyocytes. It contains one ETS DNA binding domain (aa 234-314). Within aa 125-231, mouse ETV2 shares 54% and 85% aa sequence identity with human and rat ETV2, respectively.

Long Name

ETS Translocation 2

Entrez Gene IDs

2116 (Human); 14008 (Mouse); 361544 (Rat)

Alternate Names

ER71; ER71MGC129835; ETS translocation variant 2; ets variant 2; Ets-related protein 71; ETSRP71; ETSRP71ets variant gene 2; ETV2; MGC129834