Mouse Ephrin-A2 Antibody Summary
Met1-Asn184
Accession # P52801
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Ephrin‑A2 in Rat Hippocampal Neurons. Ephrin‑A2 was detected in immersion fixed rat hippocampal neurons using 10 µg/mL Goat Anti-Mouse Ephrin‑A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF603) for 3 hours at room temperature. Neurons were stained (green) and glial cells were labeled by using anti-GFAP antibodies (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ephrin-A2
Ephrin-A2, also known as ELF-1, Cek7-L and LERK-6 (1), is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A2 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of human and mouse Ephrin-A2 share 93% aa identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression.(3) It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/Ephrin families also appear to play a role in angiogenesis (3).
- Eph Nomenclature Committee [letter] (1997) Cell 9:403.
- Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
- Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Product Datasheets
Citations for Mouse Ephrin-A2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.
Authors: Dewannieux M, Vernochet C, Ribet D, Bartosch B, Cosset FL, Heidmann T
PLoS Pathog., 2011-10-20;7(10):e1002309.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis.
Authors: Irie N, Takada Y, Watanabe Y, Matsuzaki Y, Naruse C, Asano M, Iwakura Y, Suda T, Matsuo K
J. Biol. Chem., 2009-03-19;284(21):14637-44.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
Authors: Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD
Neuron, 2008-09-11;59(5):746-58.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Immunoprecipitation
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