Mouse Ephrin-A2 Antibody

Catalog #: MAB458 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB458
MAB458-SP

All Ephrin-A2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Ephrin-A2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Ephrin-A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant mouse (rm) Ephrin-A4, recombinant human (rh) Ephrin-A3, rmEphrin-B1, and rhEphrin-B3 is observed.

Source

Monoclonal Rat IgG_2A Clone # 84019

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A2
Arg21-Asn184
Accession # P52801

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Ephrin-A2 Fc Chimera (Catalog # 603-A2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A2

Ephrin-A2, also known as ELF-1, Cek7-L and LERK-6 (1), is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A2 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of human and mouse Ephrin-A2 share 93% aa identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression.(3) It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/Ephrin families also appear to play a role in angiogenesis (3).

References

Eph Nomenclature Committee [letter] (1997) Cell 9:403.
Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Entrez Gene IDs

1943 (Human); 13637 (Mouse)

Alternate Names

Cek7-L; EFNA2; ELF-1; EPH-related receptor tyrosine kinase ligand 6; EphrinA2; Ephrin-A2; EPLG6HEK7 ligand; HEK7-L; HEK7-ligand; LERK6; LERK6LERK-6; ligand of eph-related kinase 6