Mouse EBI3 PE-conjugated Antibody Summary
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL-27/IL-35 EBI3 Subunit in Mouse splenocytes by Flow Cytometry. Mouse splenocytes untreated (open histogram) or treated with LPS (filled histogram) were stained with Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit PE-conjugated Monoclonal Antibody (Catalog # IC18341A) or isotype control antibody (Catalog # IC006P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EBI3
IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.
Product Datasheets
Citations for Mouse EBI3 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity
Authors: Bhalchandra Mirlekar, Yan Wang, Sirui Li, Mi Zhou, Sarah Entwistle, Tristan De Buysscher et al.
Cell Reports Medicine
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Human placental trophoblast cells contribute to maternal-fetal tolerance through expressing IL-35 and mediating iTR35 conversion
Authors: J Liu, S Hao, X Chen, H Zhao, L Du, H Ren, C Wang, H Mao
Nat Commun, 2019-10-10;10(1):4601.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulatory T cells and their derived cytokine, interleukin-35, reduce pain in experimental autoimmune encephalomyelitis
Authors: SS Duffy, BA Keating, CJ Perera, JG Lees, RS Tonkin, PGS Makker, P Carrive, O Butovsky, G Moalem-Tay
J. Neurosci., 2019-01-16;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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