Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody

Name: Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1525
Rating: 4 (1 reviews)

Catalog #: BAF1525
3 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF1525

All Dectin-2/CLEC6A Antibodies

Product Details

Citations (3)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Dectin‑2 alpha /CLEC6A in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human DLEC is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑2 alpha /CLEC6A
Phe43-Leu209
Accession # Q9JKF4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Dectin‑2/CLEC6A alpha Isoform (Catalog # 1525-DC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-2/CLEC6A

Dectin-2 alpha, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3‑6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4⁺CD25⁺ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2.

References

Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
Kanazawa, N. et al. (2004) Immunobiology 209:179.
Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
McGreal, E.P. et al. (2006) Glycobiology 16:422.
Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Entrez Gene IDs

93978 (Human); 56620 (Mouse)

Alternate Names

CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; Dectin2; Dectin-2; Dendritic cell-associated C-type lectin 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Dectin-2/CLEC6A alpha Isoform Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation
Authors: Giang T. Nguyen, Shuying Xu, Walter Adams, John M. Leong, Stephen C. Bunnell, Michael K. Mansour et al.
iScience
The balance between gasdermin D and STING signaling shapes the severity of schistosome immunopathology
Authors: P Kalantari, I Shecter, J Hopkins, A Pilotta Go, Y Morales, BF Harandi, S Sharma, MJ Stadecker
Proceedings of the National Academy of Sciences of the United States of America, 2023-03-21;120(13):e2211047120.
CD209a Synergizes with Dectin-2 and Mincle to Drive Severe Th17 Cell-Mediated Schistosome Egg-Induced Immunopathology
Authors: P Kalantari, Y Morales, EA Miller, LD Jaramillo, HE Ponichtera, MA Wuethrich, C Cheong, MC Seminario, JM Russo, SC Bunnell, MJ Stadecker
Cell Rep, 2018-01-30;22(5):1288-1300.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot