Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 1.09 | 2.6 | 4.1 | 1.25 | 3.15 | 4.91 |
Standard Deviation | 0.04 | 0.12 | 0.21 | 0.09 | 0.24 | 0.37 |
CV% | 3.7 | 4.6 | 5.1 | 7.2 | 7.6 | 7.5 |
Recovery
The recovery of mouse SDF-1 alpha spiked to three different levels in samples throughout the range of the assay was assessed.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 101 | 98-104 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CXCL12/SDF-1 alpha
CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
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Citations for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Smooth muscle cell-derived Cxcl12 directs macrophage accrual and sympathetic innervation to control thermogenic adipose tissue
Authors: Lee, D;Benvie, AM;Steiner, BM;Kolba, NJ;Ford, JG;McCabe, SM;Jiang, Y;Berry, DC;
Cell reports
Species: Mouse
Sample Types:
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Upregulation of IL4-induced gene 1 enzyme by B2 cells during melanoma progression impairs their antitumor properties
Authors: Bekkat, F;Seradj, M;Lengagne, R;Fiore, F;Kato, M;Lucas, B;Castellano, F;Molinier-Frenkel, V;Richard, Y;Prévost-Blondel, A;
European journal of immunology
Species: Mouse
Sample Types: Cell Lysates
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Haematopoietic stem cell numbers are not solely determined by niche availability
Authors: Takeishi, S;Marchand, T;Koba, WR;Borger, DK;Xu, C;Guha, C;Bergman, A;Frenette, PS;Gritsman, K;Steidl, U;
bioRxiv : the preprint server for biology
Species: Mouse, Transgenic Mouse
Sample Types: Bone Marrow Lavage Fluid
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Neutrophil metalloproteinase driven spleen damage hampers infection control of trypanosomiasis
Authors: Pham, HTT;Magez, S;Choi, B;Baatar, B;Jung, J;Radwanska, M;
Nature communications
Species: Mouse
Sample Types: Tissue Homogenates
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High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production
Authors: SY Heazlewood, T Ahmad, B Cao, H Cao, M Domingues, X Sun, CK Heazlewood, S Li, B Williams, M Fulton, JF White, T Nebl, CM Nefzger, JM Polo, BT Kile, F Kraus, MT Ryan, YB Sun, PFM Choong, SL Ellis, ML Anko, SK Nilsson
Nature Communications, 2023-04-13;14(1):2099.
Species: Mouse
Sample Types: Plasma
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METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling
Authors: S Luo, C Liao, L Zhang, C Ling, X Zhang, P Xie, G Su, Z Chen, L Zhang, T Lai, J Tang
Cell Reports, 2023-03-14;42(3):112259.
Species: Mouse
Sample Types: Cell Culture Supernates
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Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells
Authors: Y Omatsu, S Aiba, T Maeta, K Higaki, K Aoki, H Watanabe, G Kondoh, R Nishimura, S Takeda, UI Chung, T Nagasawa
Nature Communications, 2022-05-12;13(1):2654.
Species: Mouse
Sample Types: Tissue Homogenates
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Cytokines and Neurodegeneration in Epileptogenesis
Authors: P Wolinski, D Ksiazek-Wi, A Glabinski
Brain sciences, 2022-03-12;12(3):.
Species: Mouse
Sample Types: Tissue Homogenates
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CXCR4-dependent macrophage-to-fibroblast signaling contributes to cardiac diastolic dysfunction in heart failure with preserved ejection fraction
Authors: N Zhang, Q Ma, Y You, X Xia, C Xie, Y Huang, Z Wang, F Ye, Z Yu, X Xie
International journal of biological sciences, 2022-01-09;18(3):1271-1287.
Species: Mouse
Sample Types: Cell Culture Supernates
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CXCR7 Antagonism Reduces Acute Lung Injury Pathogenesis
Authors: L Pouzol, A Sassi, N Baumlin, M Tunis, DS Strasser, F Lehembre, MM Martinic
Oncogene, 2021-11-05;12(0):748740.
Species: Mouse
Sample Types: Plasma
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Nanoparticle-containing Electrospun Nanofibrous Scaffolds for Sustained Release of SDF-1alpha
Authors: R Molina-Peñ, M Haji Manso, M Najberg, JM Thomassin, B Gueza, C Alvarez-Lo, E Garcion, C Jérôme, F Boury
International journal of pharmaceutics, 2021-10-17;0(0):121205.
Species: Mouse
Sample Types: Cell Culture Supernates
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Interleukin-17A Triggers the Release of Platelet-Derived Factors Driving Vascular Endothelial Cells toward a Pro-Angiogenic State
Authors: A Gatsiou, K Sopova, A Tselepis, K Stellos
Cells, 2021-07-22;10(8):.
Species: Human
Sample Types: Cell Culture Supernates
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Tumor-Associated Macrophage Promotes the Survival of Cancer Cells upon Docetaxel Chemotherapy via the CSF1/CSF1R-CXCL12/CXCR4 Axis in Castration-Resistant Prostate Cancer
Authors: W Guan, F Li, Z Zhao, Z Zhang, J Hu, Y Zhang
Genes, 2021-05-19;12(5):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Adipocyte-Specific ACKR3 Regulates Lipid Levels in Adipose Tissue
Authors: S Gencer, Y Döring, Y Jansen, S Bayasgalan, O Schengel, M Müller, LJF Peters, C Weber, EPC van der Vo
Biomedicines, 2021-04-06;9(4):.
Species: Mouse
Sample Types: Plasma
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Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells
Authors: AA Hastreiter, GG Dos Santos, EN Makiyama, EWC Santos, P Borelli, RA Fock
The Journal of nutritional biochemistry, 2021-03-08;0(0):108626.
Species: Mouse
Sample Types: Cell Culture Supernates
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FGF21 promotes ischaemic angiogenesis and endothelial progenitor cells function under diabetic conditions in an AMPK/NAD+-dependent manner
Authors: Q Dai, X Fan, X Meng, S Sun, Y Su, X Ling, X Chen, K Wang, X Dai, C Zhang, S Da, G Zhang, C Gu, H Chen, J He, H Hu, L Yu, X Pan, Y Tan, X Yan
Journal of Cellular and Molecular Medicine, 2021-02-17;0(0):.
Species: Mouse
Sample Types: Plasma
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Spinal cord injury causes chronic bone marrow failure
Authors: RS Carpenter, JM Marbourg, FH Brennan, KA Mifflin, JCE Hall, RR Jiang, XM Mo, M Karunasiri, MH Burke, AM Dorrance, PG Popovich
Nat Commun, 2020-07-24;11(1):3702.
Species: Mouse
Sample Types: Bone Marrow Lysate
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Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation
Authors: Y Zhu, T Hirata, F Mackay, F Murakami
Sci Rep, 2020-07-16;10(1):11830.
Species: Mouse
Sample Types: Tissue Lysate
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Pbrm1 Steers Mesenchymal Stromal Cell Osteolineage Differentiation by Integrating PBAF-Dependent Chromatin Remodeling and BMP/TGF-&beta Signaling
Authors: S Sinha, M Biswas, SS Chatterjee, S Kumar, A Sengupta
Cell Rep, 2020-04-28;31(4):107570.
Species: Mouse
Sample Types: Cell Culture Supernates
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Myocardium-targeted transplantation of PHD2 shRNA-modified bone mesenchymal stem cells through ultrasound-targeted microbubble destruction protects the heart from acute myocardial infarction
Authors: Z Sun, Y Xie, RJ Lee, Y Chen, Q Jin, Q Lv, J Wang, Y Yang, Y Li, Y Cai, R Wang, Z Han, L Zhang, M Xie
Theranostics, 2020-04-06;10(11):4967-4982.
Species: Rat
Sample Types: Cell Culture Supernants
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Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model
Authors: CC Hsiao, CC Lin, YS Hou, JY Ko, CJ Wang
Int J Mol Sci, 2019-10-05;20(19):.
Species: Rat
Sample Types: Tissue Lysates
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Mesenchymal stem cell-derived conditioned medium attenuate angiotensin II-induced aortic aneurysm growth by modulating macrophage polarization
Authors: YZ Zhou, Z Cheng, Y Wu, QY Wu, XB Liao, Y Zhao, JM Li, XM Zhou, XM Fu
J. Cell. Mol. Med., 2019-10-04;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Hematopoietic-Extrinsic Cues Dictate Circadian Redistribution of Mature and Immature Hematopoietic Cells in Blood and Spleen
Authors: M Stenzinger, D Karpova, C Unterraine, S Harenkamp, E Wiercinska, K Hoerster, M Pfeffer, E Maronde, H Bonig
Cells, 2019-09-05;8(9):.
Species: Mouse
Sample Types: Plasma
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Evaluation of Neurosecretome from Mesenchymal Stem Cells Encapsulated in Silk Fibroin Hydrogels
Authors: Y Martín-Mar, L Fernández-, MH Sanchez-Re, N Marí-Buyé, FJ Rojo, J Pérez-Rigu, M Ramos, GV Guinea, F Panetsos, D González-N
Sci Rep, 2019-06-19;9(1):8801.
Species: Mouse
Sample Types: Cell Culture Supernates
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CXCL12-CXCR4 pathway activates brown adipocytes and induces insulin resistance in CXCR4-deficient mice under high-fat diet
Authors: K Kurita, K Ishikawa, K Takeda, M Fujimoto, H Ono, J Kumagai, H Inoue, H Yokoh, K Yokote
Sci Rep, 2019-04-16;9(1):6165.
Species: Mouse
Sample Types: Serum
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The Release of Adipose Stromal Cells from Subcutaneous Adipose Tissue Regulates Ectopic Intramuscular Adipocyte Deposition
Authors: A Girousse, M Gil-Ortega, V Bourlier, C Bergeaud, Q Sastourné-, C Moro, C Barreau, C Guissard, J Vion, E Arnaud, JP Pradère, N Juin, L Casteilla, C Sengenès
Cell Rep, 2019-04-09;27(2):323-333.e5.
Species: Mouse
Sample Types: Plasma
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Optimal design, anti-tumour efficacy and tolerability of anti-CXCR4 antibody drug conjugates
Authors: MJ Costa, J Kudaravall, JT Ma, WH Ho, K Delaria, C Holz, A Stauffer, AG Chunyk, Q Zong, E Blasi, B Buetow, TT Tran, K Lindquist, M Dorywalska, A Rajpal, DL Shelton, P Strop, SH Liu
Sci Rep, 2019-02-21;9(1):2443.
Species: Mouse
Sample Types: Serum
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JNK signalling mediates aspects of maternal immune activation: importance of maternal genotype in relation to schizophrenia risk
Authors: RL Openshaw, J Kwon, A McColl, JM Penninger, J Cavanagh, JA Pratt, BJ Morris
J Neuroinflammation, 2019-01-28;16(1):18.
Species: Mouse
Sample Types: Plasma
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Aging Suppresses Skin-Derived Circulating SDF1 to Promote Full-Thickness Tissue Regeneration
Authors: MA Nishiguchi, CA Spencer, DH Leung, TH Leung
Cell Rep, 2018-09-25;24(13):3383-3392.e5.
Species: Mouse
Sample Types: Serum
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Decellularized Diaphragmatic Muscle Drives a Constructive Angiogenic Response In Vivo
Authors: ME Alvarèz Fa, M Piccoli, C Franzin, A Sgrò, A Dedja, L Urbani, E Bertin, C Trevisan, P Gamba, AJ Burns, P De Coppi, M Pozzobon
Int J Mol Sci, 2018-04-28;19(5):.
Species: Mouse
Sample Types: Tissue Homogenates
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Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro
Authors: RM Natoli, H Yu, MC Meislin, P Abbasnia, P Roper, A Vuchkovska, X Xiao, SR Stock, JJ Callaci
J Orthop Surg Res, 2018-04-27;13(1):101.
Species: Mouse
Sample Types: Tissue Lysates
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Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
Mol. Cancer Ther., 2018-04-25;0(0):.
Species: Mouse
Sample Types: Plasma
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SDF-1 is an Autocrine Insulin-Desensitizing Factor in Adipocytes
Authors: J Shin, A Fukuhara, T Onodera, S Kita, C Yokoyama, M Otsuki, I Shimomura
Diabetes, 2018-03-26;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
A mouse model for evaluation of efficacy and concomitant toxicity of anti-human CXCR4 therapeutics
Authors: MJ Costa, J Kudaravall, WH Liu, J Stock, S Kong, SH Liu
PLoS ONE, 2018-03-19;13(3):e0194688.
Species: Mouse
Sample Types: Serum
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Anti-CD69 therapy induces rapid mobilization and high proliferation of HSPCs through S1P and mTOR
Authors: L Notario, E Alari-Pahi, A Albentosa, M Leiva, G Sabio, P Lauzurica
Leukemia, 2018-02-27;0(0):.
Species: Mouse
Sample Types: Plasma
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Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine
Authors: P Chang, B Zhang, L Shao, W Song, W Shi, L Wang, T Xu, D Li, X Gao, Y Qu, L Dong, J Wang
Cell Death Dis, 2018-02-05;9(2):154.
Species: Mouse
Sample Types: Serum
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Neuroprotective effects of ellagic acid on cuprizone-induced acute demyelination through limitation of microgliosis, adjustment of CXCL12/IL-17/IL-11 axis and restriction of mature oligodendrocytes apoptosis
Authors: N Sanadgol, F Golab, Z Tashakkor, N Taki, S Moradi Kou, A Mostafaie, M Mehdizadeh, M Abdollahi, G Taghizadeh, M Sharifzade
Pharm Biol, 2017-12-01;55(1):1679-1687.
Species: Mouse
Sample Types: Tissue Homogenates
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Prevention of breast cancer skeletal metastases with parathyroid hormone
Authors: S Swami, J Johnson, LA Bettinson, T Kimura, H Zhu, MA Albertelli, RW Johnson, JY Wu
JCI Insight, 2017-09-07;2(17):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Comprehensive comparison of three different animal models for systemic inflammation
Authors: S Seemann, F Zohles, A Lupp
J. Biomed. Sci., 2017-08-24;24(1):60.
Species: Mouse
Sample Types: Serum
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Sitagliptin-mediated preservation of endothelial progenitor cell function via augmenting autophagy enhances ischaemic angiogenesis in diabetes
Authors: X Dai, J Zeng, X Yan, Q Lin, K Wang, J Chen, F Shen, X Gu, Y Wang, J Chen, K Pan, L Cai, KA Wintergers, Y Tan
J. Cell. Mol. Med., 2017-08-10;0(0):.
Species: Mouse
Sample Types: Plasma
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Sitagliptin, a dipeptidyl peptidase-4 inhibitor, suppresses CXCL5 and SDF-1 and does not accelerate intestinal neoplasia formation in Apc(Min/+) mice fed a high-fat diet
Authors: K Fujiwara, T Inoue, Y Henmi, Y Hirata, Y Naka, A Hara, K Kakimoto, S Nouda, T Okada, K Kawakami, T Takeuchi, K Higuchi
Oncol Lett, 2017-08-01;14(4):4355-4360.
Species: Mouse
Sample Types: Plasma
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Lymphoid differentiation of hematopoietic stem cells requires efficient Cxcr4 desensitization
Authors: C Freitas, M Wittner, J Nguyen, V Rondeau, V Biajoux, ML Aknin, F Gaudin, S Beaussant-, Y Bertrand, C Bellanné-C, J Donadieu, F Bachelerie, M Espéli, A Dalloul, F Louache, K Balabanian
J. Exp. Med., 2017-05-26;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Cannabinoid CB1 receptor overactivity contributes to the pathogenesis of idiopathic pulmonary fibrosis
Authors: R Cinar, BR Gochuico, MR Iyer, T Jourdan, T Yokoyama, JK Park, NJ Coffey, H Pri-Chen, G Szanda, Z Liu, K Mackie, WA Gahl, G Kunos
JCI Insight, 2017-04-20;2(8):.
Species: Mouse
Sample Types: BALF
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Neuronal p38? mediates synaptic and cognitive dysfunction in an Alzheimer's mouse model by controlling ?-amyloid production
Authors: S Colié, S Sarroca, R Palenzuela, I Garcia, A Matheu, R Corpas, CG Dotti, JA Esteban, C Sanfeliu, AR Nebreda
Sci Rep, 2017-03-31;7(0):45306.
Species: Mouse
Sample Types: Tissue Homogenates
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Dual Affinity Heparin-Based Hydrogels Achieve Pro-Regenerative Immunomodulation and Microvascular Remodeling
Authors: ME Ogle, JR Krieger, LE Tellier, J McFaline-F, JS Temenoff, EA Botchwey
ACS Biomater Sci Eng, 2017-02-20;4(4):1241-1250.
Species: Mouse
Sample Types: Hydrogel Supernates
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Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model
Authors: J Chen, M Zhang, F Zhou, J Wang, B Niu, W Zhang
Oncol Lett, 2017-02-13;13(4):2323-2329.
Species: Mouse
Sample Types: Cell Lysates
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Sphingosine-1-phosphate Receptor-3 Supports Hematopoietic Stem and Progenitor Cell Residence within the Bone Marrow Niche
Stem Cells, 2017-01-19;0(0):.
Species: Mouse
Sample Types: Plasma
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The DPP4 Inhibitor Linagliptin Protects from Experimental Diabetic Retinopathy
PLoS ONE, 2016-12-12;11(12):e0167853.
Species: Mouse, Rat
Sample Types: Tissue Homogenates
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Absence of CCR2 results in an inflammaging environment in young mice with age-independent impairments in muscle regeneration
J Leukoc Biol, 2016-08-16;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Direct Renin Inhibition with Aliskiren Improves Ischemia-Induced Neovasculogenesis in Diabetic Animals via the SDF-1 Related Mechanism.
Authors: Chang T, Wu T, Huang P, Lin C, Chen J, Lin L, Lin S, Chen J
PLoS ONE, 2015-08-25;10(8):e0136627.
Species: Mouse
Sample Types: Plasma
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Intra-Arterial Transplantation of Allogeneic Mesenchymal Stem Cells Mounts Neuroprotective Effects in a Transient Ischemic Stroke Model in Rats: Analyses of Therapeutic Time Window and Its Mechanisms.
Authors: Toyoshima A, Yasuhara T, Kameda M, Morimoto J, Takeuchi H, Wang F, Sasaki T, Sasada S, Shinko A, Wakamori T, Okazaki M, Kondo A, Agari T, Borlongan C, Date I
PLoS ONE, 2015-06-15;10(6):e0127302.
Species: Rat
Sample Types: Tissue Homogenates
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Notch Receptor-Ligand Engagement Maintains Hematopoietic Stem Cell Quiescence and Niche Retention.
Authors: Wang W, Yu S, Zimmerman G, Wang Y, Myers J, Yu V, Huang D, Huang X, Shim J, Huang Y, Xin W, Qiao P, Yan M, Xin W, Scadden D, Stanley P, Lowe J, Huang A, Siebel C, Zhou L
Stem Cells, 2015-05-13;33(7):2280-93.
Species: Mouse
Sample Types: Cell Culture Supernates
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SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
Stem Cells Dev, 2015-02-05;24(9):1073-81.
Species: Mouse
Sample Types: Serum
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Transplanted bone marrow-derived circulating PDGFRalpha+ cells restore type VII collagen in recessive dystrophic epidermolysis bullosa mouse skin graft.
Authors: Iinuma S, Aikawa E, Tamai K, Fujita R, Kikuchi Y, Chino T, Kikuta J, McGrath J, Uitto J, Ishii M, Iizuka H, Kaneda Y
J Immunol, 2015-01-19;194(4):1996-2003.
Species: Mouse
Sample Types: Serum
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Resolving cancer-stroma interfacial signalling and interventions with micropatterned tumour-stromal assays.
Authors: Shen K, Luk S, Hicks D, Elman J, Bohr S, Iwamoto Y, Murray R, Pena K, Wang F, Seker E, Weissleder R, Yarmush M, Toner M, Sgroi D, Parekkadan B
Nat Commun, 2014-12-09;5(0):5662.
Species: Mouse
Sample Types: Tissue Homogenates
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Heparan sulfate inhibits hematopoietic stem and progenitor cell migration and engraftment in mucopolysaccharidosis I.
Authors: Watson H, Holley R, Langford-Smith K, Wilkinson F, van Kuppevelt T, Wynn R, Wraith J, Merry C, Bigger B
J Biol Chem, 2014-10-30;289(52):36194-203.
Species: Mouse
Sample Types: Complex Sample Type
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Low intensity pulsed ultrasound enhanced mesenchymal stem cell recruitment through stromal derived factor-1 signaling in fracture healing.
Authors: Wei F, Leung K, Li G, Qin J, Chow S, Huang S, Sun M, Qin L, Cheung W
PLoS ONE, 2014-09-02;9(9):e106722.
Species: Rat
Sample Types: Cell Culture Supernates
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A novel molecule Me6TREN promotes angiogenesis via enhancing endothelial progenitor cell mobilization and recruitment.
Authors: Chen H, Wang S, Zhang J, Ren X, Zhang R, Shi W, Lv Y, Zhou Y, Yan X, Chen L, He L, Zhang B, Nan X, Yue W, Li Y, Pei X
Sci Rep, 2014-08-28;4(0):6222.
Species: Mouse
Sample Types: Plasma
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Stimulation of endogenous cardioblasts by exogenous cell therapy after myocardial infarction.
Authors: Malliaras K, Ibrahim A, Tseliou E, Liu W, Sun B, Middleton R, Seinfeld J, Wang L, Sharifi B, Marban E
EMBO Mol Med, 2014-04-06;6(6):760-77.
Species: Mouse
Sample Types: Cell Culture Supernates
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IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
Authors: Fleige H, Ravens S, Moschovakis G, Bolter J, Willenzon S, Sutter G, Haussler S, Kalinke U, Prinz I, Forster R
J Exp Med, 2014-03-24;211(4):643-51.
Species: Mouse
Sample Types: Cell Culture Supernates
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C/EBPbeta mediates osteoclast recruitment by regulating endothelial progenitor cell expression of SDF-1alpha.
Authors: Fu S, Pang H, Xu J, Wu X
PLoS ONE, 2014-03-11;9(3):e91217.
Species: Mouse
Sample Types: Cell Culture Supernates
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CCAAT/enhancer-binding protein beta expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesis.
Authors: Yoshioka S, Miura Y, Yao H, Satake S, Hayashi Y, Tamura A, Hishita T, Ichinohe T, Hirai H, Takaor-Kondo A, Maekawa T
Stem Cells, 2014-03-01;32(3):730-40.
Species: Mouse
Sample Types: Cell Culture Supernates
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SDF1-a facilitates Lin-/Sca1+ cell homing following murine experimental cerebral ischemia.
Authors: Mocco J, Afzal A, Ansari S, Wolfe A, Caldwell K, Connolly E, Scott E
PLoS ONE, 2014-01-20;9(1):e85615.
Species: Mouse
Sample Types: Tissue Homogenates
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Klotho deficiency disrupts hematopoietic stem cell development and erythropoiesis.
Authors: Vadakke Madathil S, Coe L, Casu C, Sitara D
Am J Pathol, 2014-01-08;184(3):827-41.
Species: Mouse
Sample Types: Serum
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Protective effects of mesenchymal stem cells with CXCR4 up-regulation in a rat renal transplantation model.
Authors: Cao, Zhiqiang, Zhang, Geng, Wang, Fuli, Liu, Hongbao, Liu, Long, Han, Yaling, Zhang, Jian, Yuan, Jianlin
PLoS ONE, 2013-12-30;8(12):e82949.
Species: Mouse
Sample Types: Tissue Homogenates
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The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.
Authors: Hoellenriegel J, Zboralski D, Maasch C, Rosin N, Wierda W, Keating M, Kruschinski A, Burger J
Blood, 2013-11-25;123(7):1032-9.
Species: Mouse
Sample Types: Cell Culture Supernates
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Inhibition of the CXCL12/CXCR4-axis as preventive therapy for radiation-induced pulmonary fibrosis.
Authors: Shu, Hui-Kuo, Yoon, Younghyo, Hong, Samuel, Xu, Kaiming, Gao, Huiying, Hao, Chunhai, Torres-Gonzalez, Edilson, Nayra, Cardenes, Rojas, Mauricio, Shim, Hyunsuk
PLoS ONE, 2013-11-07;8(11):e79768.
Species: Mouse
Sample Types: BALF
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SDF-1alpha mediates wound-promoted tumor growth in a syngeneic orthotopic mouse model of breast cancer.
Authors: Stuelten C, Cervoni-Curet F, Busch J, Sutton E, Webster J, Kavalukas S, Wakefield L, Barbul A, Niederhuber J
PLoS ONE, 2013-04-11;8(4):e60919.
Species: Mouse
Sample Types: Tissue Secretion
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Activator protein-2alpha mediates carbon monoxide-induced stromal cell-derived factor-1alpha expression and vascularization in ischemic heart.
Authors: Lin H, Chen Y, Chiang M, Huang P, Chau L
Arterioscler Thromb Vasc Biol, 2013-02-07;33(4):785-94.
Species: Mouse
Sample Types: Tissue Homogenates
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Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.
Authors: Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y
Br. J. Cancer, 2011-10-20;105(10):1615-24.
Species: Mouse
Sample Types: Serum
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JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.
Authors: Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M
Blood, 2011-08-25;118(17):4609-19.
Species: Mouse
Sample Types: Cell Culture Supernates
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Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence.
Authors: Oladipupo SS, Hu S, Santeford AC, Yao J, Kovalski JR, Shohet RV, Maslov K, Wang LV, Arbeit JM
Blood, 2011-02-09;117(15):4142-53.
Species: Human, Mouse
Sample Types: Plasma, Tissue Homogenates
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Application of the chemokine CXCL12 expression plasmid restores wound healing to near normal in a diabetic mouse model.
Authors: Restivo TE, Mace KA, Harken AH, Young DM
J Trauma, 2010-08-01;69(2):392-8.
Species: Mouse
Sample Types: Serum
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Novel insight into stem cell mobilization-plasma sphingosine-1-phosphate is a major chemoattractant that directs the egress of hematopoietic stem progenitor cells from the bone marrow and its level in peripheral blood increases during mobilization due to activation of complement cascade/membrane attack complex.
Authors: Ratajczak MZ, Lee H, Wysoczynski M, Wan W, Marlicz W, Laughlin MJ, Kucia M, Janowska-Wieczorek A, Ratajczak J
Leukemia, 2010-04-01;24(5):976-85.
Species: Mouse
Sample Types: Plasma
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CXCR4 and CXCR7 regulate angiogenesis and CT26.WT tumor growth independent from SDF-1.
Authors: Kollmar O, Rupertus K, Scheuer C, Nickels RM, Haberl GC, Tilton B, Menger MD, Schilling MK
Int. J. Cancer, 2010-03-15;126(6):1302-15.
Species: Mouse
Sample Types: Serum
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Podocytes produce homeostatic chemokine stromal cell-derived factor-1/CXCL12, which contributes to glomerulosclerosis, podocyte loss and albuminuria in a mouse model of type 2 diabetes.
Authors: Sayyed SG, Hagele H, Kulkarni OP, Endlich K, Segerer S, Eulberg D, Klussmann S, Anders HJ
Diabetologia, 2009-08-26;52(11):2445-54.
Species: Mouse
Sample Types: Plasma
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Cell-nonautonomous function of Id1 in the hematopoietic progenitor cell niche.
Authors: Suh HC, Ji M, Gooya J, Lee M, Klarmann KD, Keller JR
Blood, 2009-05-28;114(6):1186-95.
Species: Mouse
Sample Types: Serum
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Synergy between CD26/DPP-IV inhibition and G-CSF improves cardiac function after acute myocardial infarction.
Authors: Zaruba MM, Theiss HD, Vallaster M, Mehl U, Brunner S, David R, Fischer R, Krieg L, Hirsch E, Huber B, Nathan P, Israel L, Imhof A, Herbach N, Assmann G, Wanke R, Mueller-Hoecker J, Steinbeck G, Franz WM
Cell Stem Cell, 2009-04-03;4(4):313-23.
Species: Mouse
Sample Types: Tissue Homogenates
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Endothelial nitric oxide synthase promotes bone marrow stromal cell migration to the ischemic myocardium via upregulation of stromal cell-derived factor-1alpha.
Authors: Li N, Lu X, Zhao X, Xiang FL, Xenocostas A, Karmazyn M, Feng Q
Stem Cells, 2009-04-01;27(4):961-70.
Species: Mouse
Sample Types: Tissue Homogenates
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The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells.
Authors: Feng W, McCabe NP, Mahabeleshwar GH, Somanath PR, Phillips DR, Byzova TV
J. Cell Biol., 2008-12-15;183(6):1145-57.
Species: Mouse
Sample Types: Plasma
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Rosuvastatin reduces atherosclerotic lesions and promotes progenitor cell mobilisation and recruitment in apolipoprotein E knockout mice.
Authors: Schroeter MR, Humboldt T, Schafer K, Konstantinides S
Atherosclerosis, 2008-11-25;205(1):63-73.
Species: Mouse
Sample Types: Plasma
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Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
Cancer Cell, 2008-09-09;14(3):263-73.
Species: Mouse
Sample Types: Plasma
-
Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
Eur. J. Pharmacol., 2008-06-16;590(1):400-8.
Species: Mouse
Sample Types: Tissue Homogenates
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Heme oxygenase-1 promotes neovascularization in ischemic heart by coinduction of VEGF and SDF-1.
Authors: Lin HH, Chen YH, Chang PF, Lee YT, Yet SF, Chau LY
J. Mol. Cell. Cardiol., 2008-05-02;45(1):44-55.
Species: Mouse
Sample Types: Tissue Homogenates
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Altered SDF-1/CXCR4 axis in patients with primary myelofibrosis and in the Gata1 low mouse model of the disease.
Authors: Migliaccio AR, Martelli F, Verrucci M, Migliaccio G, Vannucchi AM, Ni H, Xu M, Jiang Y, Nakamoto B, Papayannopoulou T, Hoffman R
Exp. Hematol., 2008-02-01;36(2):158-71.
Species: Mouse
Sample Types: Plasma
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Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74.
Species: Mouse
Sample Types: Plasma
-
Pulmonary stromal-derived factor-1 expression and effect on neutrophil recruitment during acute lung injury.
Authors: Petty JM, Sueblinvong V, Lenox CC, Jones CC, Cosgrove GP, Cool CD, Rai PR, Brown KK, Weiss DJ, Poynter ME, Suratt BT
J. Immunol., 2007-06-15;178(12):8148-57.
Species: Mouse
Sample Types: BALF
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Hypoxia-induced mitogenic factor has proangiogenic and proinflammatory effects in the lung via VEGF and VEGF receptor-2.
Authors: Yamaji-Kegan K, Su Q, Angelini DJ, Champion HC, Johns RA
Am. J. Physiol. Lung Cell Mol. Physiol., 2006-08-04;291(6):L1159-68.
Species: Mouse, Rat
Sample Types: Cell Culture Supernates
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Enalapril increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system.
Authors: Wang CH, Verma S, Hsieh IC, Chen YJ, Kuo LT, Yang NI, Wang SY, Wu MY, Hsu CM, Cheng CW, Cherng WJ
J. Mol. Cell. Cardiol., 2006-05-05;41(1):34-43.
Species: Mouse
Sample Types: Plasma
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Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
Authors: Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A
Blood, 2004-09-28;105(3):931-9.
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
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Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand.
Authors: Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S
Cell, 2002-05-31;109(5):625-37.
Species: Mouse
Sample Types: Plasma
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We isolated mouse tumor tissues and collected serum samples to explore changes in the immune microenvironment of the TME and changes in immune-related indicators in the blood system.
Cell culture supernatant from RAW2647 cells stimulated with LPS and LPS+MSC-EV
We have used this kit to check SDF gradient in mouse plasma and Bone marrow supernatant. Our results indicated that SDF gradient was shifted in favor of Bone marrow after treatment.
Very good precision and results are reliable. Published some data already and going to publish more.