Mouse Cripto Antibody Summary
Arg26-Gln150
Accession # P51865
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cripto in D3 Mouse Cell Line. Cripto was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse Cripto Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1538) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cripto
Cripto is the founding member of the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes. These developmental processes include: formation of the germ layers and dorsal organizer, specification of anterior-posterior and left-right axes, and differentiation of heart muscle (1, 2). Other members of the EGF-CFC family include Cryptic, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead). Overall sequence identity between members of the family is low, but they do share several common domains: a variant EGF-like motif, a novel conserved
cysteine‑rich domain (called CFC domain), and a C-terminal hydrophobic region. Most EGF-CFC members have a glycosyl-phosphatidylinositol (GPI) anchoring site at the C-terminus and exist as extracellular membrane-anchored proteins. However, naturally-occurring soluble isoforms also exist. Mouse Cripto shares 66% and 34% amino acid identity with human Cripto and zebrafish OEP, respectively (2). Despite weak conservation in amino acid identity, EGF-CFC family members appear to function similarly in assays for phenotypic rescue of zebrafish oep mutants (2). Both secreted and membrane bound forms of Cripto demonstrate biological activity (3).
Cripto, also known as CFC-2 or TDGF-1 (teratocarcinoma-derived growth factor), was originally isolated from an undifferentiated human teratocarcinoma cell line as a potential oncogene. It is overexpressed in many types of cancers and acts as a growth factor for tumors (4). Genetic evidence from mice and zebrafish points to a role for Cripto as an essential cofactor in Nodal signaling. Cripto and OEP mutants display defects in mesoderm induction and heart morphogenesis, similar to phenotypes seen in Nodal mutants (2).
Cripto acts as a cofactor for Nodal by recruiting the Activin type I Receptor, ALK-4, leading to an Act RIIB-ALK4-Cripto-Nodal complex for signaling (1, 3). Cripto also forms a complex with activin and Act RIIs to block activin signaling (5). Work has shown that other TGF-beta superfamily members such as Vg1 and GDF-1 also require EGF-CFC cofactors (6). Cripto can also activate mitogen-activated protein kinase (MAPK) and Akt pathways independently of Nodal by directly binding to a membrane-associated heparan sulfate proteoglycan, glypican-1 (7).
- Rosa, F.M. (2002) Science’s STKE http://stke.sciencemag.org/.
- Shen, M. and A. Schier (2000) Trends Genet. 16:303.
- Yan, Y-T. et al. (2002) Mol. Cell Biol. 22:4439.
- Salomon, D. et al. (2000) Endocrine-Rel. Cancer 7:199.
- Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
- Cheng, S. et al. (2003) Genes & Dev. 17:31.
- Bianco, C. et al. (2003) Cancer Research 63:1192.
Product Datasheets
Citations for Mouse Cripto Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Biomanufacturing Recombinantly Expressed Cripto-1 Protein in Anchorage-Dependent Mammalian Cells Growing in Suspension Bioreactors within a Three-Dimensional Hydrogel Microcarrier
Authors: Rachel Lev, Orit Bar-Am, Yoni Lati, Ombretta Guardiola, Gabriella Minchiotti, Dror Seliktar
Gels
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CRIPTO Is a Marker of Chemotherapy-Induced Stem Cell Expansion in Non-Small Cell Lung Cancer
Authors: Federica Francescangeli, Maria Laura De Angelis, Rachele Rossi, Giovanni Sette, Adriana Eramo, Alessandra Boe et al.
Frontiers in Oncology
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Conditional Cripto overexpression in satellite cells promotes myogenic commitment and enhances early regeneration
Authors: Carolina Prezioso, Salvatore Iaconis, Gennaro Andolfi, Lorena Zentilin, Francescopaolo Iavarone, Ombretta Guardiola et al.
Frontiers in Cell and Developmental Biology
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Defects in GPI biosynthesis perturb Cripto signaling during forebrain development in two new mouse models of holoprosencephaly
Authors: David M. McKean, Lee Niswander
Biology Open
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