Mouse Collagen II Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Collagen II antibody in Mouse Chondrocytes by Immunocytochemistry (ICC). Collagen II antibody in Mouse Chondrocytes by Immunocytochemistry (ICC).](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Collagen_II_AF3615_Immunocytochemistry_6496.jpg)
Collagen II in Mouse Chondrocytes. Collagen II was detected in immersion fixed mouse mesenchymal stem cell-differentiated chondrocytes using 10 µg/mL Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3615) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen II
Mouse Type II Collagen is a 100 kDa fibrillar collagen found in cartilage, vitreous body and vertebral disk. It is synthesized as a 1459 amino acid (aa) preproprecursor that contains an N-terminal and a C-terminal proregion. Intracellularly, type II Collagen homotrimerizes into a fibril and is then secreted. The proregions are then cleaved by procollagenases and the processed homotrimer covalently crosslinks to other type II fibrils. Mature mouse Collagen II shares 95% and 98% aa sequence identity with human and rat Collagen II, respectively.
Product Datasheets
Citations for Mouse Collagen II Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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SOST Deficiency Aggravates Osteoarthritis in Mice by Promoting Sclerosis of Subchondral Bone
Authors: J Li, J Xue, Y Jing, M Wang, R Shu, H Xu, C Xue, J Feng, P Wang, D Bai
Biomed Res Int, 2019-11-11;2019(0):7623562.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse
Authors: Y Chen, H Xu, G Lin
Cell Discov, 2017-12-19;3(0):17046.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Bone marrow-derived multipotent stromal cells attenuate inflammation in obliterative airway disease in mouse tracheal allografts.
Authors: Casey A, Dirks F, Liang O, Harrach H, Schuette-Nuetgen K, Leeman K, Kim C, Gerard C, Subramaniam M
Stem Cells Int, 2014-09-10;2014(0):468927.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-P -
Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjogren's-like disease.
Authors: Khalili S, Liu Y, Kornete M, Roescher N, Kodama S, Peterson A, Piccirillo CA, Tran SD
PLoS ONE, 2012-06-07;7(6):e38615.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription.
Authors: Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A
PLoS ONE, 2012-04-12;7(4):e34703.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is necessary for normal endochondral ossification and aggrecan metabolism.
Authors: Sato T, Kudo T, Ikehara Y, Ogawa H, Hirano T, Kiyohara K, Hagiwara K, Togayachi A, Ema M, Takahashi S, Kimata K, Watanabe H, Narimatsu H
J. Biol. Chem., 2010-12-10;286(7):5803-12.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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