Mouse Coagulation Factor III/TF DuoSet ELISA

Catalog #: DY3178-05
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY3178-05

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

Mouse Coagulation Factor III / Tissue Factor ELISA Standard Curve

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All Coagulation Factor III/Tissue Factor ELISAs

Product Details

Citations (3)

Supplemental Products

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse Coagulation Factor III/TF DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

23.4 - 1,500 pg/mL

Sufficient Materials

For five 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Coagulation Factor III / Tissue Factor. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Mouse Coagulation Factor III / Tissue Factor ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.

Entrez Gene IDs:

2152 (Human); 14066 (Mouse)

Alternate Names:

CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor

Citations for Mouse Coagulation Factor III/TF DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Hutchinson-Gilford progeria syndrome mice display accelerated arterial thrombus formation and increased platelet reactivity
Authors: Puspitasari, YM;Ministrini, S;Han, J;Karch, C;Prisco, F;Liberale, L;Bengs, S;Akhmedov, A;Montecucco, F;Beer, JH;Lüscher, TF;Bongiovanni, D;Camici, GG;
Thrombosis research
Species: Transgenic Mouse
Sample Types: Plasma, Tissue Homogenates
A TLR4-independent critical role for CD14 in intracellular LPS sensing
Authors: SO Vasudevan, AJ Russo, P Kumari, SK Vanaja, VA Rathinam
Cell Reports, 2022-05-03;39(5):110755.
Species: Mouse
Sample Types: Plasma
Loss of Sirt3 accelerates arterial thrombosis by increasing formation of neutrophil extracellular traps and plasma tissue factor activity
Authors: DS Gaul, J Weber, LJ Van Tits, S Sluka, L Pasterk, MF Reiner, N Calatayud, C Lohmann, R Klingenber, J Pahla, D Vdovenko, FC Tanner, GG Camici, U Eriksson, J Auwerx, F Mach, S Windecker, N Rodondi, TF L Uuml Sch, S Winnik, CM Matter
Cardiovasc. Res., 2018-07-01;0(0):.
Species: Mouse
Sample Types: Plasma