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Mouse CD99 PE-conjugated Antibody

Catalog #: FAB3905P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3905P
Detection of CD99 in Mouse Splenocytes by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD99 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD99 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) PILR‑ alpha and rmPILR‑ beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD99
Gly25-Leu138
Accession # Q8VCN6
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD99 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD99 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CD99 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3905P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD99

CD99, also known as PILR-L and MIC2, is a 125 amino acid (aa), 24‑30 kDa glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. CD99 has been implicated in processes like homotypic cell adhesion, diapedesis and oncosuppression. The extracellular domain of the mouse CD99 protein used as immunogen has 60% aa sequence identity to rat CD99 and 43% aa sequence identity to the human CD99.

Entrez Gene IDs
4267 (Human); 673094 (Mouse); 652929 (Rat)
Alternate Names
12E7; CD99 antigenY homolog; CD99 molecule; CD99; E2 antigen; HBA71; MIC2 (monoclonal 12E7); MIC2; MIC2Y; MSK5X; pilr-1; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2

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Citations for Mouse CD99 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cancer-associated mutations in the canonical cleavage site do not influence CD99 shedding by the metalloprotease meprin beta but alter cell migration in vitro
    Authors: Tillmann Bedau, Neele Schumacher, Florian Peters, Johannes Prox, Philipp Arnold, Tomas Koudelka et al.
    Oncotarget
  2. Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin ? and promotes transendothelial cell migration
    Authors: Tillmann Bedau
    FASEB J, 2016-12-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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