Mouse CD69 Antibody Summary
Gly64-Arg199
Accession # P37217
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD69 in mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight vs unstimulated mouse splenocytes by Flow Cytometry Mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight (A) vs unstimulated mouse splenocytes (B) were stained with Goat Anti-Mouse CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2386) and Rat Anti-Mouse CD3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4841F) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD69
CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets. The C-terminal extracellular domain of mouse CD69 shares approximately 57% amino acid sequence homology with the corresponding region of human CD69.
Product Datasheets
Citations for Mouse CD69 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Lymph node conduits transport virions for rapid T cell activation
Authors: Glennys V. Reynoso, Andrea S. Weisberg, John P. Shannon, Daniel T. McManus, Lucas Shores, Jeffrey L. Americo et al.
Nature Immunology
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CD8+ T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging
Authors: T Kaya, N Mattugini, L Liu, H Ji, L Cantuti-Ca, J Wu, M Schifferer, J Groh, R Martini, S Besson-Gir, S Kaji, A Liesz, O Gokce, M Simons
Nature Neuroscience, 2022-10-24;25(11):1446-1457.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Differentiation Paths of Peyer's Patch LysoDCs Are Linked to Sampling Site Positioning, Migration, and T Cell Priming
Authors: C Wagner, J Bonnardel, C Da Silva, L Spinelli, CA Portilla, J Tomas, M Lagier, L Chasson, M Masse, M Dalod, A Chollat-Na, JP Gorvel, H Lelouard
Cell Rep, 2020-04-07;31(1):107479.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Dynamic Reorganization of Chromatin Accessibility Signatures during Dedifferentiation of Secretory Precursors into Lgr5+ Intestinal Stem Cells
Authors: U Jadhav, M Saxena, NK O'Neill, A Saadatpour, GC Yuan, Z Herbert, K Murata, RA Shivdasani
Cell Stem Cell, 2017-06-22;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
CXCR3 chemokine receptor enables local CD8(+) T cell migration for the destruction of virus-infected cells.
Authors: Hickman H, Reynoso G, Ngudiankama B, Cush S, Gibbs J, Bennink J, Yewdell J
Immunity, 2015-03-10;42(3):524-37.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
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Cryosections from PLP immersion fixed PyMT mammary gland tumors were stained for CD69 (1:100) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody for 1h at RT. CD3 in green, CD69 in red, nuclear staining (Hoechst) in blue.
