Mouse CD47 N-terminal IgV-like Extracellular Domain Biotinylated Antibody

Catalog #: BAF1866
1 Citation Datasheet / COA / SDS

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BAF1866

All CD47 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD47 N-terminal IgV-like Extracellular Domain Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD47 N-terminal IgV-like Extracellular Domain in Western blots. In Western blots, approximately 15% cross‑reactivity with recombinant human CD47 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like Extracellular Domain
Gln19-Pro158
Accession # NP_034711

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD47 N-terminal IgV-like Extracellular Domain

Flow Cytometry

0.25 µg/10⁶ cells

Mouse splenocytes

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse ovary and thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD47

CD47, also known as integrin-associated protein, is an integral membrane protein with a single IgV-like domain at its N-terminus followed by five membrane-spanning regions. CD47 is expressed ubiquitously. It is a ligand for the transmembrane signal regulatory protein alpha 1 (SIRP alpha ) and also functions as a receptor for thrombospondin.

Entrez Gene IDs

961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)

Alternate Names

antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse CD47 N-terminal IgV-like Extracellular Domain Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

CD47 is a negative regulator of intestinal epithelial cell self-renewal following DSS-induced experimental colitis
Authors: Y He, X Sun, W Rong, R Yang, H Liang, Y Qi, L Li, K Zen
Sci Rep, 2020-06-23;10(1):10180.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot