Mouse CD45 APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD45 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD45 APC-conjugated Monoclonal Antibody (Catalog # FAB114A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Background: CD45
CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The mouse CD45RABC cDNA encodes 1291 amino acids (aa), including a 23 aa signal sequence, a 541 aa extracellular domain containing the splicing region, a cysteine-rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 705 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 30‑169. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).
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- Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
- Ralph, S.J. et al. (1987) EMBO J. 6:1251.
- Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
- Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
- McNiell, L. et al. (2007) Immunity 27:425.
- Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
- Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
- Zhu, J.W. et al. (2008) Immunity 28:183.
- Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
- Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
- Cross, J.L. et al. (2008) J. Immunol. 180:8020.
- Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
Product Datasheets
Citations for Mouse CD45 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Keap1/Nrf2/Ho-1 Pathway
Authors: Latifa Mohamed Abdelgawad, Manar Mohy Abd El-hamed, Dina Sabry, Marwa Abdelgwad, Department of Medical Laser applications, National Institute of laser enhanced science, Cairo University, Egypt., Department of Medical Laser application, National Institute of laser enhanced science, Cairo University, Egypt et al.
Reports of Biochemistry and Molecular Biology
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Influence of photobiomodulation and vitamin D on osteoblastic differentiation of human periodontal ligament stem cells and bone-like tissue formation through enzymatic activity and gene expression
Authors: Latifa Mohamed Abdelgawad, Asmaa Mohamed Abdelaziz, Dina Sabry, Marwa Abdelgwad
Biomolecular Concepts
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CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology
Authors: M Felsenstei, A Blank, AD Bungert, A Mueller, A Ghori, I Kremenetsk, O Rung, T Broggini, K Turkowski, L Scherschin, J Raggatz, P Vajkoczy, S Brandenbur
Cancers (Basel), 2020-07-13;12(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The neovascularization effect of dedifferentiated fat cells
Authors: H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto
Sci Rep, 2020-06-08;10(1):9211.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-12 promotes myeloid-derived suppressor cell recruitment and bacterial persistence during Staphylococcus aureus orthopedic implant infection.
Authors: Heim C, Vidlak D, Scherr T, Hartman C, Garvin K, Kielian T
J Immunol, 2015-03-11;194(8):3861-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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