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Mouse CD45 Antibody

Catalog #: MAB114
24 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB114-SP
MAB114-500
MAB114-100
CD45 in Mouse Splenocytes.
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Citations (24)
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Mouse CD45 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD45. Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15).
Source
Monoclonal Rat IgG2B Clone # 30-F11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse thymus and spleen cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of mouse spleen
Immunoprecipitation
Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
 
Complement-dependent Cytotoxicity
Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
 
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-25 µg/mL
Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

CD45 in Mouse Splenocytes. CD45 was detected in immersion fixed mouse splenocytes (positive staining) and Neuro‑2A mouse neuroblastoma cell line (negative staining) using Rat Anti-Mouse CD45 Monoclonal Antibody (Catalog # MAB114) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry View Larger

CD45 in Mouse Spleen. CD45 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse CD45 Monoclonal Antibody (Catalog # MAB114) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The mouse CD45RABC cDNA encodes 1291 amino acids (aa), including a 23 aa signal sequence, a 541 aa extracellular domain containing the splicing region, a cysteine-rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 705 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode
aa 30‑169. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References
  1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
  2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
  3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
  5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
  6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
  7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
  8. McNiell, L. et al. (2007) Immunity 27:425.
  9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
  10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
  11. Zhu, J.W. et al. (2008) Immunity 28:183.
  12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
  13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
  14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
  15. Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names
B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200

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Citations for Mouse CD45 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema
    Authors: GD García Nor, CL Ly, DA Cuzzone, RP Kataru, GE Hespe, JS Torrisi, JJ Huang, JC Gardenier, IL Savetsky, MD Nitti, JZ Yu, S Rehal, BJ Mehrara
    Nat Commun, 2018-05-17;9(1):1970.
  2. The extracellular matrix of lymph node reticular fibers modulates follicle border interactions and germinal center formation
    Authors: Jian Song, Tushar Deshpande, Xueli Zhang, Melanie-Jane Hannocks, Nils Lycke, Susanna L. Cardell et al.
    iScience
  3. Dual targeting of mTOR/IL-17A and autophagy by fisetin alleviates psoriasis-like skin inflammation
    Authors: Tithi Roy, Sergette Banang-Mbeumi, Samuel T. Boateng, Emmanuelle M. Ruiz, Roxane-Cherille N. Chamcheu, Lin Kang et al.
    Frontiers in Immunology
  4. TGF‐ beta 1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation
    Authors: Jung Eun Baik, Hyeung Ju Park, Raghu P. Kataru, Ira L. Savetsky, Catherine L. Ly, Jinyeon Shin et al.
    Clinical and Translational Medicine
  5. TnP Peptide Suppresses Experimental Autoimmune Encephalomyelitis (EAE) in a Preclinical Mouse Model
    Authors: Carla Lima, Adolfo Luis Almeida Maleski, Jefferson Thiago Gonçalves Bernardo, Vitor Cataldi Zelli, Evilin Naname Komegae, Monica Lopes-Ferreira
    Frontiers in Immunology
  6. Rubicon-regulated beta-1 adrenergic receptor recycling protects the heart from pressure overload
    Authors: Y Akazawa, M Taneike, H Ueda, R Kitazume-T, T Murakawa, R Sugihara, H Yorifuji, H Nishida, K Mine, A Hioki, S Omiya, H Nakayama, O Yamaguchi, T Yoshimori, Y Sakata, K Otsu
    Scientific Reports, 2022-01-07;12(1):41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Fasting and fasting-mimicking treatment activate SIRT1/LXR alpha and alleviate diabetes-induced systemic and microvascular dysfunction
    Authors: Sandra S. Hammer, Cristiano P. Vieira, Delaney McFarland, Maximilian Sandler, Yan Levitsky, Tim F. Dorweiler et al.
    Diabetologia
  8. Impact of Preanalytical Factors During Histology Processing on Section Suitability for Digital Image Analysis
    Authors: Elizabeth A. Chlipala, Mark Butters, Miles Brous, Jessica S. Fortin, Roni Archuletta, Karen Copeland et al.
    Toxicologic Pathology
  9. Deficiency in Aim2 affects viability and calcification of vascular smooth muscle cells from murine aortas and angiotensin-II induced aortic aneurysms
    Authors: Markus Wortmann, Muhammad Arshad, Maani Hakimi, Dittmar Böckler, Susanne Dihlmann
    Molecular Medicine
  10. Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm
    Authors: SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh
    Exp. Mol. Med., 2020-09-14;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Cytokine mRNA Degradation in Cardiomyocytes Restrains Sterile Inflammation in Pressure-Overloaded Hearts
    Authors: Shigemiki Omiya, Yosuke Omori, Manabu Taneike, Tomokazu Murakawa, Jumpei Ito, Yohei Tanada et al.
    Circulation
  12. Tumor Lymphatic Function Regulates Tumor Inflammatory and Immunosuppressive Microenvironments
    Authors: Raghu P. Kataru, Catherine L. Ly, Jinyeon Shin, Hyeung Ju Park, Jung Eun Baik, Sonia Rehal et al.
    Cancer Immunology Research
  13. T helper 2 differentiation is necessary for development of lymphedema
    Authors: Catherine L. Ly, Gabriela D. García García Nores, Raghu P. Kataru, Babak J. Mehrara
    Translational Research
  14. UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease
    Authors: L Elia, P Kunderfran, P Carullo, M Vacchiano, FM Farina, IF Hall, S Mantero, C Panico, R Papait, G Condorelli, M Quintavall
    J. Clin. Invest., 2018-05-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Combination of CD40 agonism and CSF-1R blockade reconditions tumor-associated macrophages and drives potent antitumor immunity
    Authors: KR Wiehagen, NM Girgis, DH Yamada, AA Smith, SR Chan, IS Grewal, M Quigley, RI Verona
    Cancer Immunol Res, 2017-11-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis
    Authors: EN Komegae, TA Souza, LZ Grund, C Lima, M Lopes-Ferr
    PLoS ONE, 2017-02-24;12(2):e0171796.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Growth inhibition of formed corneal neovascularization following Fosaprepitant treatment
    Authors: F Bignami, A Lorusso, P Rama, G Ferrari
    Acta Ophthalmol, 2017-02-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Toll-like receptor 9 prevents cardiac rupture after myocardial infarction in mice independently of inflammation
    Authors: Shigemiki Omiya, Yosuke Omori, Manabu Taneike, Andrea Protti, Osamu Yamaguchi, Shizuo Akira et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  19. Diphtheria toxin–mediated ablation of lymphatic endothelial cells results in progressive lymphedema
    Authors: Jason C. Gardenier, Geoffrey E. Hespe, Raghu P. Kataru, Ira L. Savetsky, Jeremy S. Torrisi, Gabriela D. García Nores et al.
    JCI Insight
  20. Induction of KIAA1199/CEMIP is associated with colon cancer phenotype and poor patient survival
    Authors: Stephen P. Fink, Lois L. Myeroff, Revital Kariv, Petra Platzer, Baozhong Xin, Debra Mikkola et al.
    Oncotarget
  21. A GRHL3-regulated repair pathway suppresses immune-mediated epidermal hyperplasia
    Authors: William M. Gordon, Michael D. Zeller, Rachel H. Klein, William R. Swindell, Hsiang Ho, Francisco Espetia et al.
    Journal of Clinical Investigation
  22. Nucleosomes contribute to increase mesangial cell chemokine expression during the development of lupus nephritis.
    Authors: Kanapathippillai P, Hedberg A, Fenton C, Fenton K
    Cytokine, 2013-04-04;62(2):244-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
    J Neuroinflammation, 2012-08-10;9(0):193.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
    Authors: Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R
    Cardiovasc. Res., 2004-09-01;63(4):617-24.
    Species: Porcine
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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