Mouse CCR7 Antibody Summary
Accession # NP_031745
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
CCR7 in Mouse Splenocytes. CCR7 was detected in immersion fixed non-stimulated mouse splenocytes using 25 µg/mL Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCR7 Antibody. Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND50 is typically 2-10 µg/mL.
Detection of CCR7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR7
CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.
Product Datasheets
Citations for Mouse CCR7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
18
Citations: Showing 1 - 10
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Tenascin-C Orchestrates an Immune-Suppressive Tumor Microenvironment in Oral Squamous Cell Carcinoma
Authors: Caroline Spenlé, Thomas Loustau, Devadarssen Murdamoothoo, William Erne, Stephanie Beghelli-de la Forest Divonne, Romain Veber et al.
Cancer Immunology Research
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Eluted 25-hydroxyvitamin D3 from radially aligned nanofiber scaffolds enhances cathelicidin production while reducing inflammatory response in human immune system-engrafted mice
Authors: Chen S, Ge L, Wang H et al.
Acta Biomater
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Cells‐Micropatterning Biomaterials for Immune Activation and Bone Regeneration
Authors: Bingjun Zhang, Fei Han, Yufeng Wang, Yuhua Sun, Meng Zhang, Xiaopeng Yu et al.
Advanced Science
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Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics
Authors: Juana Serrano-Lopez, Shailaja Hegde, Sachin Kumar, Josefina Serrano, Jing Fang, Ashley M Wellendorf et al.
eLife
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Skewed epithelial cell differentiation and premature aging of the thymus in the absence of vitamin D signaling
Authors: Artusa, P;Nguyen Yamamoto, L;Barbier, C;Valbon, SF;Aghazadeh Habashi, Y;Djambazian, H;Ismailova, A;Lebel, MÈ;Salehi-Tabar, R;Sarmadi, F;Ragoussis, J;Goltzman, D;Melichar, HJ;White, JH;
Science advances
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Functionalized nanowires for miRNA-mediated therapeutic programming of naïve T cells
Authors: Yee Mon, KJ;Kim, S;Dai, Z;West, JD;Zhu, H;Jain, R;Grimson, A;Rudd, BD;Singh, A;
Nature nanotechnology
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-33 promotes sciatic nerve regeneration in mice by modulating macrophage polarization
Authors: Wasman Smail, S;Ziyad Abdulqadir, S;Omar Khudhur, Z;Elia Ishaq, S;Faqiyazdin Ahmed, A;Ghayour, MB;Abdolmaleki, A;
International immunopharmacology
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity
Authors: J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao
Cell Reports, 2023-01-18;42(1):111991.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Contribution of Heparan Sulphate Binding in CCL21-Mediated Migration of Breast Cancer Cells
Authors: I Del Molino, A Meeson, K Cooke, MI Malki, B Barron-Mil, JA Kirby, S Ali
Cancers, 2021-07-10;13(14):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense
Authors: FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang
Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Ligand-competent fractalkine receptor is expressed on exosomes
Authors: EJ Park, PK Myint, MG Appiah, P Worawattan, J Inprasit, O Prajuabjin, ZY Soe, A Gaowa, E Kawamoto, M Shimaoka
Biochemistry and Biophysics Reports, 2021-02-02;26(0):100932.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Lymphangiogenesis in kidney and lymph node mediates renal inflammation and fibrosis
Authors: G Pei, Y Yao, Q Yang, M Wang, Y Wang, J Wu, P Wang, Y Li, F Zhu, J Yang, Y Zhang, W Yang, X Deng, Z Zhao, H Zhu, S Ge, M Han, R Zeng, G Xu
Sci Adv, 2019-06-26;5(6):eaaw5075.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis.
Authors: Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J
Oncogene, 2015-05-11;35(6):748-60.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
FOXO1 regulates dendritic cell activity through ICAM-1 and CCR7.
Authors: Dong G, Wang Y, Xiao W, Pacios Pujado S, Xu F, Tian C, Xiao E, Choi Y, Graves D
J Immunol, 2015-03-18;194(8):3745-55.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
Authors: Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P
Infect Immun, 2014-05-12;82(8):3098-112.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma.
Authors: Irino, Tomoyuki, Takeuchi, Hiroya, Matsuda, Sachiko, Saikawa, Yoshiro, Kawakubo, Hirofumi, Wada, Norihito, Takahashi, Tsunehir, Nakamura, Rieko, Fukuda, Kazumasa, Omori, Tai, Kitagawa, Yuko
BMC Cancer, 2014-04-26;14(0):291.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development -
Direct B cell stimulation by dendritic cells in a mouse model of lupus.
Authors: Wan S, Zhou Z, Duan B, Morel L
Arthritis Rheum., 2008-06-01;8(0):1741-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Coexpression of CCR7 and CXCR4 During B Cell Development Controls CXCR4 Responsiveness and Bone Marrow Homing
Authors: Saria Mcheik, Nils Van Eeckhout, Cédric De Poorter, Céline Galés, Marc Parmentier, Jean-Yves Springael
Frontiers in Immunology
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