Mouse CCL28 Antibody

Catalog #: AF533
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF533
AF533-SP

Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody.

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All CCL28 Antibodies

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Citations (5)

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Mouse CCL28 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL28 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human CCL28 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CCL28 (Catalog # 533-VI)

Neutralization

Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND₅₀) is typically 5-25 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL28.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody. Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL28 (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF533). The ND₅₀ is typically 5-25 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL28

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.

References

Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Entrez Gene IDs

56477 (Human); 56838 (Mouse)

Alternate Names

CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC

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Citations for Mouse CCL28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control.
Authors: Cha HR, Ko HJ, Kim ED
J. Immunol., 2011-08-10;187(6):3044-52.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010-02-08;120(3):907-23.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
J. Immunol., 2009-06-01;182(11):6851-60.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
Authors: Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
J. Immunol., 2004-09-15;173(6):3668-75.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
Authors: Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
J. Immunol., 2003-02-01;170(3):1452-61.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr