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Mouse CCL11/Eotaxin Antibody

Catalog #: AF-420-NA
23 Citations 3 Reviews Datasheet / COA / SDS
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AF-420-NA
AF-420-SP
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Chemotaxis Induced by CCL11/Eotaxin and Neutral-ization by Mouse CCL11/ Eotaxin Antibody.
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Citations (23)
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Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL11/Eotaxin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human CCL11/Eotaxin is observed, and less than 5% cross-reactivity with recombinant mouse JE is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL11/Eotaxin
His24-Pro97
Accession # P48298
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)
Immunohistochemistry
5-15 µg/mL
See below

Mouse CCL11/Eotaxin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL11/Eotaxin Biotinylated Antibody (Catalog # BAF420)

Standard: Recombinant Mouse CCL11/Eotaxin Protein (Catalog # 420-ME)

Neutralization
Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL11/Eotaxin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL11/Eotaxin and Neutral-ization by Mouse CCL11/ Eotaxin Antibody. View Larger

Chemotaxis Induced by CCL11/Eotaxin and Neutral-ization by Mouse CCL11/ Eotaxin Antibody. Recombinant Mouse CCL11/ Eotaxin (Catalog # 420-ME) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL11/ Eotaxin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA). The ND50 is typically 0.1-0.5 µg/mL.

Immunohistochemistry CCL11/Eotaxin antibody in Mouse Colon by Immunohistochemistry (IHC-Fr). View Larger

CCL11/Eotaxin in Mouse Colon. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.

References
  1. Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
  2. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
  3. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  4. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
Entrez Gene IDs
6356 (Human); 20292 (Mouse)
Alternate Names
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

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Citations for Mouse CCL11/Eotaxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Alendronate Attenuates Eosinophilic Airway Inflammation Associated with Suppression of Th2 Cytokines, Th17 Cytokines, and Eotaxin-2
    Authors: Oh Sasaki, Mitsuru Imamura, Yusuke Yamazumi, Hiroaki Harada, Taku Matsumoto, Katsuhide Okunishi et al.
    The Journal of Immunology
  2. Eosinophils are an essential element of a type 2 immune axis that controls thymus regeneration
    Authors: Emilie J. Cosway, Andrea J. White, Sonia M. Parnell, Edina Schweighoffer, Helen E. Jolin, Andrea Bacon et al.
    Science Immunology
  3. Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis
    Authors: HS Choi, T Won, X Hou, G Chen, W Bracamonte, MV Talor, I Jur?ová, O Szárszoi, L ?urnova, I St?íž, JE Hooper, V Melenovský, D ?iháková
    Cell Rep, 2020-03-03;30(9):2989-3003.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. A role for endothelial cells in radiation-induced inflammation
    Authors: M Boström, M Kalm, Y Eriksson, C Bull, A Ståhlberg, T Björk-Erik, N Hellström, K Blomgren
    Int. J. Radiat. Biol., 2018-02-07;0(0):1-36.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Modulation of the diet and gastrointestinal microbiota normalizes systemic inflammation and ?-cell chemokine expression associated with autoimmune diabetes susceptibility
    Authors: AM Henschel, SM Cabrera, ML Kaldunski, S Jia, R Geoffrey, MF Roethle, V Lam, YG Chen, X Wang, NH Salzman, MJ Hessner
    PLoS ONE, 2018-01-02;13(1):e0190351.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. CCL11, a novel mediator of inflammatory bone resorption
    Authors: E Kindstedt, CK Holm, R Sulniute, I Martinez-C, R Lundmark, P Lundberg
    Sci Rep, 2017-07-13;7(1):5334.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart
    Eur J Immunol, 2016-10-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions.
    Authors: Chao G, Wallis R, Marandi L, Ning T, Sarmiento J, Paterson A, Poussier P
    J Immunol, 2014-03-19;192(8):3645-53.
    Species: Rat
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  9. Suppression of laser-induced choroidal neovascularization by a CCR3 antagonist.
    Authors: Mizutani, Takeshi, Ashikari, Masayuki, Tokoro, Mayumi, Nozaki, Miho, Ogura, Yuichiro
    Invest Ophthalmol Vis Sci, 2013-02-28;54(2):1564-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  11. The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
    Authors: Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T
    Nature, 2011-08-31;477(7362):90-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
    Authors: De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA
    Eur. Respir. J., 2009-10-19;35(6):1377-87.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. CCR3 is a target for age-related macular degeneration diagnosis and therapy.
    Authors: Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J
    Nature, 2009-06-14;460(7252):225-30.
    Species: Mouse
    Sample Types: Vitreous Humor
    Applications: Neutralization
  15. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  16. IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice.
    Authors: Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA
    J. Clin. Invest., 2007-10-01;117(10):2941-51.
    Species: Mouse
    Sample Types: Serum
  17. Toll-like receptor-2-mediated C-C chemokine receptor 3 and eotaxin-driven eosinophil influx induced by Mycobacterium bovis BCG pleurisy.
    Authors: D&amp;apos;Avila H, Almeida PE, Roque NR, Castro-Faria-Neto HC, Bozza PT
    Infect. Immun., 2006-12-11;75(3):1507-11.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  19. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  20. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
    Authors: Reading PC, Symons JA, Smith GL
    J. Immunol., 2003-02-01;170(3):1435-42.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  21. P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
    Authors: Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S
    J. Leukoc. Biol., 2003-01-01;73(1):65-73.
    Species: Mouse
    Sample Types: Peritoneal Fluid
    Applications: ELISA Development
  22. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002-04-01;160(4):1353-60.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  23. Ablation of Tumor Progression Locus 2 Promotes a Type 2 Th Cell Response in Ovalbumin-Immunized Mice
    Authors: Wendy T. Watford, Chun-Chi Wang, Christos Tsatsanis, Lisa A. Mielke, Aristides G. Eliopoulos, Constantine Daskalakis et al.
    The Journal of Immunology

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Reviews for Mouse CCL11/Eotaxin Antibody

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Mouse CCL11/Eotaxin Antibody
By Katie Myers on 11/28/2022
Application: ELISA Sample Tested: Serum Species: Mouse

Worked as capture antibody

Mouse CCL11/Eotaxin Antibody
By Anonymous on 08/07/2019
Application: ELISA Sample Tested: Serum Species: Mouse
ELISA Mouse CCL11/Eotaxin Antibody AF-420-NA
Mouse CCL11/Eotaxin Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Rat pancreatic islet lysate

Rat pancreatic islet lysate were blotted overnight in 4 degree with polyclonal goat anti-mouse Ccl11. Serial dilution of lysate in the firs 2 lanes followed by 2 lanes of recombinant rat Ccl11.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1/1000

Western Blot Mouse CCL11/Eotaxin Antibody AF-420-NA