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Mouse BAFF/BLyS/TNFSF13B Antibody

Catalog #: AF2106
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2106
AF2106-SP
Cell Proliferation Induced by BAFF/BLyS/TNFSF13B and Neutralization by Mouse BAFF/BLyS/TNFSF13B Antibody.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BAFF/BLyS/TNFSF13B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) BAFF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BAFF/BLyS/TNFSF13B
Ala127-Leu309
Accession # Q9WU72
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 2106-BF)
Neutralization
Measured by its ability to neutralize BAFF/BLyS/TNFSF13B-induced proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 0.01-0.04 µg/mL in the presence of 3 ng/mL Recombinant Mouse BAFF/BLyS/TNFSF13B and Goat F(ab')2 Anti-mouse IgM.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by BAFF/BLyS/TNFSF13B and Neutralization by Mouse BAFF/BLyS/TNFSF13B Antibody. View Larger

Cell Proliferation Induced by BAFF/BLyS/TNFSF13B and Neutralization by Mouse BAFF/BLyS/TNFSF13B Antibody. In the presence of Goat F(ab')2 Anti-mouse IgM, Recombinant Mouse BAFF/BLyS/ TNFSF13B (Catalog # 8876-BF) stimulates pro-liferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Goat Anti-Mouse BAFF/BLyS/TNFSF13B (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2106). The ND50 is typically 0.01-0.04 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAFF/BLyS/TNFSF13B

BAFF (also known as TALL-1, BLyS, THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 aa protein consisting of a 248 amino acid (aa) extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6-8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is upregulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

References
  1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
  3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145. 
  4. Liu, Y. et al. (2002) Cell 108:383. 
  5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313. 
  6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  7. Thompson, J.S. et al. (2001) Science 293:2108.
  8. Ng, L. G. et al. (2004) J. Immunol. 173:807.
  9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
Long Name
B cell Activating Factor
Entrez Gene IDs
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

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Citations for Mouse BAFF/BLyS/TNFSF13B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis
    J Exp Med, 2016-07-18;0(0):.
  2. Venous-plexus-associated lymphoid hubs support meningeal humoral immunity
    Authors: Fitzpatrick, Z;Ghabdan Zanluqui, N;Rosenblum, JS;Tuong, ZK;Lee, CYC;Chandrashekhar, V;Negro-Demontel, ML;Stewart, AP;Posner, DA;Buckley, M;Allinson, KSJ;Mastorakos, P;Chittiboina, P;Maric, D;Donahue, D;Helmy, A;Tajsic, T;Ferdinand, JR;Portet, A;Peñalver, A;Gillman, E;Zhuang, Z;Clatworthy, MR;McGavern, DB;
    Nature
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Neutrophils license iNKT cells to regulate self-reactive mouse B cell responses
    Nat. Immunol., 2016-10-31;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2015-07-03;30(1):163-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Functional implication of BAFF synthesis and release in gangliosides-stimulated microglia.
    Authors: Kim KS, Park JY, Jou I, Park SM
    J. Leukoc. Biol., 2009-04-30;86(2):349-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. An Inflammatory Loop Between Spleen-Derived Myeloid Cells and CD4+ T Cells Leads to Accumulation of Long-Lived Plasma Cells That Exacerbates Lupus Autoimmunity
    Authors: Eunkyeong Jang, Somi Cho, Sungjin Pyo, Jin-Wu Nam, Jeehee Youn
    Frontiers in Immunology
  7. Incorporating B cell activating factor (BAFF) into the membrane of rabies virus (RABV) particles improves the speed and magnitude of vaccine-induced antibody responses
    Authors: Joseph R. Plummer, James P. McGettigan
    PLOS Neglected Tropical Diseases
  8. Neutrophils drive type I interferon production and autoantibodies in patients with Wiskott-Aldrich syndrome
    Authors: Karla E. Cervantes-Luevano, Nicoletta Caronni, Maria C. Castiello, Elena Fontana, Giulia M. Piperno, Asma Naseem et al.
    Journal of Allergy and Clinical Immunology

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