Mouse B220/CD45R mFluor™ Violet 450-Conjugated Antibody

Catalog #: FAB1217MFV450 Datasheet / COA / SDS

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FAB1217MFV450-100

Detection of B220/CD45R in Mouse splenocytes by Flow Cytometry

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Mouse B220/CD45R mFluor™ Violet 450-Conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse B220/CD45R.

Source

Monoclonal Rat IgG_2A Clone # RA3-6B2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Abelson mouse leukemia virus-induced pre-B tumor cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

mFluor Violet 450 SE (Excitation= 406 nm, Emission= 445 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.1-1 µg/10⁶ cells

Mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of B220/CD45R in Mouse splenocytes by Flow Cytometry Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB1217MFV450) or (B) unstained. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

References

Coffman, R.L. (1982) Immuno. Rev. 69:5.
Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
Allman, D.M. et al. (1992) J. Immunol. 149:2533.
Asensi, V.K. et al. (1989) Immunology 68:204.
Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.

Long Name

Cluster of Differentiation 45

Entrez Gene IDs

5788 (Human); 19264 (Mouse); 24699 (Rat)

Alternate Names

B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide