Mouse B220/CD45R Fluorescein-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B220/CD45R
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
- Coffman, R.L. (1982) Immuno. Rev. 69:5.
- Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
- Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
- Allman, D.M. et al. (1992) J. Immunol. 149:2533.
- Asensi, V.K. et al. (1989) Immunology 68:204.
- Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Product Datasheets
Citations for Mouse B220/CD45R Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK.
Authors: Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M
PLoS ONE, 2015-04-24;10(4):e0125340.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Nematode Asparaginyl-tRNA Synthetase Resolves Intestinal Inflammation in Mice with T-Cell Transfer Colitis
Authors: Michael A. Kron, Ahmed Metwali, Sanja Vodanovic-Jankovic, David Elliott
Clinical and Vaccine Immunology
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Infection with Anaplasma phagocytophilum induces multilineage alterations in hematopoietic progenitor cells and peripheral blood cells.
Authors: Johns JL, Macnamara KC, Walker NJ, Winslow GM, Borjesson DL
Infect. Immun., 2009-06-29;77(9):4070-80.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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The single cell suspension was isolated from a spleen of an IL1-R KO (knockout) mouse and stained with the FITC-Conjugated CD45R antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer.
