Mouse B220/CD45R APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of B220/CD45R antibody in Mouse Splenocytes antibody by Flow Cytometry. Detection of B220/CD45R antibody in Mouse Splenocytes antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/B220_FAB1217A_Flow_Cytometry_17838.jpg)
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and A) Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) or B) RatIgG2AAPC-conjugated isotype control antibody (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B220/CD45R
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
- Coffman, R.L. (1982) Immuno. Rev. 69:5.
- Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
- Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
- Allman, D.M. et al. (1992) J. Immunol. 149:2533.
- Asensi, V.K. et al. (1989) Immunology 68:204.
- Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Product Datasheets
Citation for Mouse B220/CD45R APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
J. Immunol., 2011-03-07;186(8):4967-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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