Home / B220/CD45R / Mouse B220/CD45R Antibody

Mouse B220/CD45R Antibody

Catalog #: MAB1217
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1217
MAB1217-SP
B220/CD45R in Mouse Splenocytes.
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Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse B220/CD45R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B220/CD45R.
Source
Monoclonal Rat IgG2A Clone # RA3-6B2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation
Coffman, R.L. (1982) Immuno. Rev. 69:5.
 
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry B220/CD45R antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry Detection of B220/CD45R antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

References
  1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
  2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
  3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
  4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
  5. Asensi, V.K. et al. (1989) Immunology 68:204.
  6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 24699 (Rat)
Alternate Names
B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide

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Citations for Mouse B220/CD45R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Positron emission tomography and single photon emission computed tomography imaging of tertiary lymphoid structures during the development of lupus nephritis
    Authors: Esmaeil S Dorraji, Ana Oteiza, Samuel Kuttner, Montserrat Martin-Armas, Premasany Kanapathippillai, Sara Garbarino et al.
    Int J Immunopathol Pharmacol
  2. Obesity Impairs Lymphatic Fluid Transport and Dendritic Cell Migration to Lymph Nodes
    Authors: Evan S. Weitman, Seth Z. Aschen, Gina Farias-Eisner, Nicholas Albano, Daniel A. Cuzzone, Swapna Ghanta et al.
    PLoS ONE
  3. Coronavirus-specific antibody production in middle-aged mice requires phospholipase A2G2D
    Authors: Jian Zheng, David Meyerholz, Lok-Yin Roy Wong, Michael Gelb, Makoto Murakami, Stanley Perlman
    Journal of Clinical Investigation
  4. B cells are not essential for Lactobacillus-mediated protection against lethal pneumovirus infection*
    Authors: Caroline M. Percopo, Kimberly D. Dyer, Katia E. Garcia-Crespo, Stanislaw J. Gabryszewski, Arthur L. Shaffer, Joseph B. Domachowske et al.
    The Journal of Immunology
  5. The extracellular matrix of lymph node reticular fibers modulates follicle border interactions and germinal center formation
    Authors: Jian Song, Tushar Deshpande, Xueli Zhang, Melanie-Jane Hannocks, Nils Lycke, Susanna L. Cardell et al.
    iScience
  6. Mast Cells Are the Trigger of Small Vessel Disease and Diastolic Dysfunction in Diabetic Obese Mice
    Authors: Sarah Guimbal, Lauriane Cornuault, Paul Rouault, Pierre-Louis Hollier, Candice Chapouly, Marie-Lise Bats et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  7. Morphometric Analysis of the Thymic Epithelial Cell (TEC) Network Using Integrated and Orthogonal Digital Pathology Approaches
    Authors: Lagou, MK;Argyris, DG;Vodopyanov, S;Gunther-Cummins, L;Hardas, A;Poutahidis, T;Panorias, C;DesMarais, S;Entenberg, C;Carpenter, RS;Guzik, H;Nishku, X;Churaman, J;Maryanovich, M;DesMarais, V;Macaluso, FP;Karagiannis, GS;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  8. Partial Mural Cell Ablation Disrupts Coronary Vasculature Integrity and Induces Systolic Dysfunction
    Authors: Cornuault, L;Hérion, FX;Bourguignon, C;Rouault, P;Foussard, N;Alzieu, P;Chapouly, C;Gadeau, AP;Couffinhal, T;Renault, MA;
    Journal of the American Heart Association
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Fine-tuning of MEK signaling is pivotal for limiting B and T�cell activation
    Authors: N Houde, L Beuret, A Bonaud, SP Fortier-Be, K Truchon-La, R Aoidi, É Pic, N Alouche, V Rondeau, G Schlecht-L, K Balabanian, M Espéli, J Charron
    Cell Reports, 2022-01-11;38(2):110223.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Kindlin-3 maintains marginal zone B cells but confines follicular B cell activation and differentiation
    Authors: A Härzschel, L Li, PW Krenn, E Szenes-Nag, G Andrieux, E Bayer, D Pfeifer, L Polcik, U Denk, JP Höpner, E Karabatak, DJ Danner, S Tangermann, L Kenner, H Jumaa, R Greil, M Börries, R Ruppert, PC Maity, TN Hartmann
    Journal of leukocyte biology, 2021-12-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Tertiary lymphoid organs are associated with the progression of kidney damage and regulated by interleukin-17A
    Authors: R Luo, Y Cheng, D Chang, T Liu, L Liu, G Pei, N Zhang, Z Wang, K Guo, W Chen, M Li, L Fan, C Zhang, Y Li, W Dai, M Zuo, Y Xu, Y Yao, S Ge, G Xu
    Theranostics, 2021-01-01;11(1):117-131.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Combined immunotherapy with anti-PDL-1/PD-1 and anti-CD4 antibodies cures syngeneic disseminated neuroblastoma
    Authors: V Rigo, L Emionite, A Daga, S Astigiano, MV Corrias, C Quintarell, F Locatelli, S Ferrini, M Croce
    Sci Rep, 2017-10-25;7(1):14049.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
    Sci Rep, 2016-08-22;6(0):31874.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. NOTCH1 mediates a switch between two distinct secretomes during senescence
    Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
    Nat. Cell Biol, 2016-08-15;18(9):979-92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. B-cell-derived IL-10 does not vitally contribute to the clinical course of glomerulonephritis.
    Authors: Kluger M, Ostmann A, Luig M, Meyer M, Goerke B, Paust H, Meyer-Schwesinger C, Stahl R, Panzer U, Tiegs G, Steinmetz O
    Eur J Immunol, 2013-12-16;44(3):683-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
    Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
    Virology, 2008-10-16;382(1):20-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Small changes in lymphocyte development and activation in mice through tissue-specific alteration of heparan sulphate.
    Authors: Garner OB, Yamaguchi Y, Esko JD, Videm V
    Immunology, 2008-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans
    Authors: Felix A. Dingler, Meng Wang, Anfeng Mu, Christopher L. Millington, Nina Oberbeck, Sam Watcham et al.
    Molecular Cell
  19. Temporal inhibition of autophagy reveals segmental reversal of ageing with increased cancer risk
    Authors: Liam D. Cassidy, Andrew R. J. Young, Christopher N. J. Young, Elizabeth J. Soilleux, Edward Fielder, Bettina M. Weigand et al.
    Nature Communications

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