Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate

Name: Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate
Brand: R&D Systems
SKU: ES002
Rating: 5 (2 reviews)

Catalog #: ES002
13 Citations 2 Reviews Datasheet / COA / SDS

Substrate for MMP-3, MMP-10, Trypsin, HGF Activator, and Coagulation Factor X

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ES002

R&D Systems Fluorogenic Peptide Substrates

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Product Details

Citations (13)

FAQs

Supplemental Products

Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Related Research Areas

Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate Summary

Product Specifications

Purity

>95%, by HPLC.

Activity

The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence. It is an excellent substrate for MMP-3 (stromelysin 1) and MMP-10 (stromelysin 2). The cleavage site is the peptide bond between Glu and Nval (norvaline).

It is also an excellent substrate for Trypsin, HGF activator and factor Xa.

Source

Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH₂
Mca: (7-Methoxycoumarin-4-yl)acetyl, Nval: Norvaline, Dnp: 2, 4-Dinitrophenyl.

Predicted Molecular Mass

1676 Da

Product Datasheets

ES002

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ES002

Formulation	Supplied as a stock solution in dimethyl sulfoxide (DMSO) at a concentration of 10.0 mg/mL.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.

Reconstitution Calculator

Related Research Areas

Citations for Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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PRSS2 remodels the tumor microenvironment via repression of Tsp1 to stimulate tumor growth and progression
Authors: L Sui, S Wang, D Ganguly, TP El Rayes, C Askeland, A Børretzen, D Sim, OJ Halvorsen, G Knutsvik, J Arnes, S Aziz, S Haukaas, WD Foulkes, DR Bielenberg, A Ziemys, V Mittal, RA Brekken, LA Akslen, RS Watnick
Nature Communications, 2022-12-27;13(1):7959.
Species: Human
Sample Types: Protein
Applications: Bioassay
A Genus-Wide Bioactivity Analysis of Daboia (Viperinae: Viperidae) Viper Venoms Reveals Widespread Variation in Haemotoxic Properties
Authors: B Op den Bro, FCP Coimbra, NR Casewell, SA Ali, FJ Vonk, BG Fry
International Journal of Molecular Sciences, 2021-12-15;22(24):.
Species: Snake
Sample Types: Venom
Applications: Bioassay
Design and synthesis of selective and blood-brain barrier-permeable hydroxamate-based gelatinase inhibitors
Authors: A Bertran, D Khomiak, A Konopka, E Rejmak, E Bulska, J Seco, L Kaczmarek, T Tarragó, R Prades
Bioorg. Chem., 2019-10-18;0(0):103365.
Species: Human
Sample Types: Protein
Applications: Bioassay
Inhibition of Human Kallikrein 5 Protease by Triterpenoids from Natural Sources
Authors: Y Matsubara, T Matsumoto, J Koseki, A Kaneko, S Aiba, K Yamasaki
Molecules, 2017-10-27;22(11):.
Protease-inhibitor interaction predictions: Lessons on the complexity of protein-protein interactions
Authors: N Fortelny, GS Butler, CM Overall, P Pavlidis
Mol. Cell Proteomics, 2017-04-06;0(0):.
Applications: Bioassay
Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage.
Authors: Zhao L, Hua T, Crowley C, Ru H, Ni X, Shaw N, Jiao L, Ding W, Qu L, Hung L, Huang W, Liu L, Ye K, Ouyang S, Cheng G, Liu Z
Cell Res, 2014-01-10;24(3):344-58.
Species: Mouse
Sample Types: Protein
Proteolytic activation cascade of the Netherton syndrome-defective protein, LEKTI, in the epidermis: implications for skin homeostasis.
Authors: Fortugno P, Bresciani A, Paolini C, Pazzagli C, El Hachem M, D'Alessio M, Zambruno G
J Invest Dermatol, 2011-06-23;131(11):2223-32.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay
The extracellular matrix protein fibronectin is a substrate for kallikrein 7.
Authors: Ramani VC, Haun RS
Biochem. Biophys. Res. Commun., 2008-03-14;369(4):1169-73.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Crystal structure of human epidermal kallikrein 7 (hK7) synthesized directly in its native state in E. coli: insights into the atomic basis of its inhibition by LEKTI domain 6 (LD6).
Authors: Fernandez IS, Standker L, Magert HJ, Forssmann WG, Gimenez-Gallego G, Romero A
J. Mol. Biol., 2008-02-09;377(5):1488-97.
Species: Human
Sample Types:
Applications: Bioassay
Autosomal ichthyosis with hypotrichosis syndrome displays low matriptase proteolytic activity and is phenocopied in ST14 hypomorphic mice.
Authors: List K, Currie B, Scharschmidt TC, Szabo R, Shireman J, Molinolo A, Cravatt BF, Segre J, Bugge TH
J. Biol. Chem., 2007-10-16;282(50):36714-23.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Microparticles of human atherosclerotic plaques enhance the shedding of the tumor necrosis factor-alpha converting enzyme/ADAM17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1.
Authors: Canault M, Leroyer AS, Peiretti F, Leseche G, Tedgui A, Bonardo B, Alessi MC, Boulanger CM, Nalbone G
Am. J. Pathol., 2007-09-14;171(5):1713-23.
Species: Human
Sample Types: Biofilm
Applications: Bioassay

Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
Cancer Cell, 2006-07-01;10(1):39-50.
Species: Human
Sample Types: Protein

Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
Genes Dev., 2006-02-15;20(5):543-56.
Species: Human
Sample Types: Buffer