Lactacystin
Chemical Name: (2R,3S,4R)-3-Hydroxy-2-[(1S)-1-hydroxy-2-methylpropyl]-4-methyl-5-oxo-2-pyrrolidinecarboxy-N-acetyl-L-cysteine thioester
Purity: ≥95%
Biological Activity
Lactacystin is a cell-permeable, potent and selective proteasome inhibitor. A Streptomyces metabolite that is thought to bind irreversibly to the active site N-terminal threonine residue of the catalytic β-subunit of the 20S proteasome, thereby inhibiting its chymotrypsin and trypsin-like activities. Induces neurite outgrowth in Neuro 2a neuroblastoma cells and has been reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC50 = 10 μM).Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Heparan Sulfate Proteoglycans Promote Telomerase Internalization and MHC Class II Presentation on Dendritic Cells
J Immunol, 2016;197(5):1597-608. -
Lactacystin, a novel microbial metabolite, induces neuritogenesis of neuroblastoma cells.
Omura et al.
J.Antibiot., 1991;44:113 -
Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin.
Fenteany et al.
Science, 1995;268:726 -
Inhibition of NF-κB activity induces apoptosis in murine hepatocytes.
Bellas et al.
Am.J.Pathol., 1997;151:891
Product Datasheets
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Citations for Lactacystin
The citations listed below are publications that use Tocris products. Selected citations for Lactacystin include:
6 Citations: Showing 1 - 6
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Evaluation of Machine Learning Classifiers to Predict Compound Mechanism of Action When Transferred across Distinct Cell Lines.
Authors: Warchal Et al.
SLAS Discov 2019;24:224
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The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Authors: Saldate Et al.
J Biol Chem 2018;293:2232
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FBG1 Is the Final Arbitrator of A1AT-Z Degradation.
Authors: Wen Et al.
PLoS One 2015;10:e0135591
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Glucocorticoid regulates TrkB protein levels via c-Cbl dependent ubiquitination: a decrease in c-Cbl mRNA in the prefrontal cortex of suicide subjects.
Authors: Pandya Et al.
Psychoneuroendocrinology 2014;45:108
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A critical role for protein degradation in the nucleus accumbens core in cocaine reward memory.
Authors: Ren Et al.
Neuropsychopharmacology 2013;38:778
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CDK5 is essential for soluble amyloid β-induced degradation of GKAP and remodeling of the synaptic actin cytoskeleton.
Authors: Roselli Et al.
PLoS One 2011;6:e23097
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