Janelia Fluor® 646, Azide

Catalog # Availability Size / Price Qty
7088/2
Janelia Fluor® 646, Azide | Click-Reactive Janelia Fluor Dyes
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Description: Red dye (Reactive group: azide). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Alternative Names: JF 646

Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethyl)carbamoyl)benzoate

Purity: ≥95%

Product Details
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Biological Activity

Key Information: Janelia Fluor® 646, Azide is a red fluorogenic fluorescent dye, supplied with an azide reactive handle for copper-free click chemistry. Suitable for live cell imaging.

Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Optical Data for Janelia Fluor® 646, Azide

Janelia Fluor<sup>®</sup> 646, Azide Dye Spectra
Brightness
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Reactive Group Azide
Reactivity Click
Cell Permeable Yes
Correction Factor 280 0.19
Application dSTORM, STED, Confocal Microscopy
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
696.88
Formula:
C37H44N6O6Si
Solubility:
Soluble to 50 mM in DMF
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

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