Janelia Fluor® 549, Tetrazine
Chemical Name: 3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Biological Activity
Key Information: Janelia Fluor® 549, Tetrazine is a yellow fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Janelia Fluor® 549, tetrazine is cell permeable. It can be coupled directly to a protein of interest using bioorthogonal chemistry and unnatural amino acid technology and has been used in this way to label living primary neurons.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Optical Data for Janelia Fluor® 549, Tetrazine
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Tetrazine |
| Reactivity | Copper-free click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal Microscopy, dSTORM, STED, Live-cell Imaging |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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Citations for Janelia Fluor® 549, Tetrazine
The citations listed below are publications that use Tocris products. Selected citations for Janelia Fluor® 549, Tetrazine include:
2 Citations: Showing 1 - 2
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Visualizing the disordered nuclear transport machinery in situ.
Authors: Yu Et al.
Nature 2023;617:162
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Minimal genetically encoded tags for fluorescent protein labeling in living neurons.
Authors: Arsic Et al.
Nat.Commun. 2022;13
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