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Human VDR/NR1I1 Antibody

Catalog #: PP-H4537-00
2 Citations Datasheet / COA / SDS
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PP-H4537-00
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VDR/NR1I1 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human VDR and cross reacts with mouse and rat VDR. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # H4537
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human VDR/NR1I1
aa 91-210
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL under reducingconditions with the appropriate secondary reagents to detect human VDR.
 
Immunohistochemistry
This antibody can be used at 20 - 40 μg/mL withthe appropriate secondary reagents to detect human VDR.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Direct ELISA
This antibody can be used at 0.1 μg/mL with the appropriatesecondary reagents to detect human VDR.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: VDR/NR1I1

Vitamin D Receptor (VDR; NR1I1) is a member of the Steroid Receptor family related to the Pregnane X Receptor (PXR) and Constitutive Androstane Receptors (CARs). The natural ligand of VDR is 1, 25 di-hydroxyvitamin D3. VDR is expressed in osteoblasts, osteocytes, osteoclasts, bone, bone marrow, thymus and small intestine. VDR plays critical roles in calcium homeostasis, bone development and mineralization, as well as control of cell growth and differentiation. Retnoid X Receptors (RXRs) are the major partners for VDR since by heterodimerizing with VDR, they increase their DNA-binding affinity and select the correct spacing of direct repeat elements.

Long Name
Vitamin D [1,25-Dihydroxyvitamin D3] Receptor
Entrez Gene IDs
7421 (Human)
Alternate Names
NR1I1; NR1I1Nuclear receptor subfamily 1 group I member 11,25-dihydroxyvitamin D3 receptor; VDR; vitamin D1,25- dihydroxyvitamin D3 receptor; vitamin D3 receptor

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Citations for Human VDR/NR1I1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Response of Npt2a knockout mice to dietary calcium and phosphorus
    Authors: Y Li, D Caballero, J Ponsetto, A Chen, C Zhu, J Guo, M Demay, H Jüppner, C Bergwitz
    PLoS ONE, 2017-04-27;12(4):e0176232.
    Species: Mouse
    Sample Types: Serum
  2. Cutting edge: progesterone directly upregulates vitamin d receptor gene expression for efficient regulation of T cells by calcitriol.
    Authors: Thangamani S, Kim M, Son Y, Huang X, Kim H, Lee J, Cho J, Ulrich B, Broxmeyer H, Kim C
    J Immunol, 2014-12-29;194(3):883-6.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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