Name: Human TWEAK R/TNFRSF12 Antibody
Brand: R&D Systems
SKU: AF1199
Price: 479 USD

Species Reactivity

Human

Specificity

Detects human TWEAK R/TNFRSF12 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse TWEAK R/TNFRSF12 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

recombinant human TWEAK R/TNFRSF12
Glu28-Trp79
Accession # Q9NP84

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199-TW)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human liver cancer tissue

Neutralization

Measured by its ability to neutralize TWEAK R/TNFRSF12-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.3-1.2 µg/mL in the presence of 30 ng/mL Recombinant Human TWEAK R/TNFRSF12 Fc Chimera and 20 ng/mL Recombinant Human TWEAK/TNFSF12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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TWEAK R/TNFRSF12 Inhibition of TWEAK/TNFSF12-depend-ent Cell Proliferation and Neutralization by Human TWEAK R/TNFRSF12 Anti-body. Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199-TW) inhibits Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human TWEAK/TNFSF12 (20 ng/mL) activity elicited by Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1199). The ND₅₀ is typically 0.3-1.2 µg/mL.

Immunohistochemistry-Paraffin

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Detection of Human TWEAK R/TNFRSF12 by Immunohistochemistry-Paraffin Expression of TWEAK and Fn14 in renal biopsies from IgAN patients was examine. Immunohistochemistry for both TWEAK (A, B) and Fn14 (D, E) were detected in glomerular crescents (the areas delineated by the squares). In the controls [renal biopsies from patients with minimal change disease (C, F)], there was very slight staining for TWEAK and Fn14 in glomeruli, while intense staining was observed in renal tubular cells. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25885587), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry-Paraffin

View Larger

Detection of Human TWEAK R/TNFRSF12 by Immunohistochemistry-Paraffin Expression of TWEAK and Fn14 in renal biopsies from IgAN patients was examine. Immunohistochemistry for both TWEAK (A, B) and Fn14 (D, E) were detected in glomerular crescents (the areas delineated by the squares). In the controls [renal biopsies from patients with minimal change disease (C, F)], there was very slight staining for TWEAK and Fn14 in glomeruli, while intense staining was observed in renal tubular cells. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25885587), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry-Paraffin

View Larger

Detection of Human TWEAK R/TNFRSF12 by Immunohistochemistry-Paraffin Expression of TWEAK and Fn14 in renal biopsies from IgAN patients was examine. Immunohistochemistry for both TWEAK (A, B) and Fn14 (D, E) were detected in glomerular crescents (the areas delineated by the squares). In the controls [renal biopsies from patients with minimal change disease (C, F)], there was very slight staining for TWEAK and Fn14 in glomeruli, while intense staining was observed in renal tubular cells. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25885587), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry-Paraffin

View Larger

Detection of Human TWEAK R/TNFRSF12 by Immunohistochemistry-Paraffin Expression of TWEAK and Fn14 in renal biopsies from IgAN patients was examine. Immunohistochemistry for both TWEAK (A, B) and Fn14 (D, E) were detected in glomerular crescents (the areas delineated by the squares). In the controls [renal biopsies from patients with minimal change disease (C, F)], there was very slight staining for TWEAK and Fn14 in glomeruli, while intense staining was observed in renal tubular cells. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25885587), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TWEAK R/TNFRSF12

The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1‑3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2‑6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

References

Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
Feng, S. et al. (2000) Am J. Pathol. 156:1253.
Wiley, S. et al. (2001) Immunity 15:837.
Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
Nakayama, M. et al. (2002) J. Immunol. 168:734.
Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.

Long Name

TNF-like and Weak Inducer of Apoptosis Receptor

Entrez Gene IDs

51330 (Human); 27279 (Mouse)

Alternate Names

CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14