Human TRA-1-60(R) PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60(R) PE-conjugated Monoclonal Antibody (Catalog # FAB4770P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRA-1-60(R)
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2-4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).
- Andrews, P. et al. (1984) Hybridoma 3:347.
- Thomson, J. et al. (1998) Science 282:1145.
- Giwercman, A. et al. (1993) Cancer 72:1308.
- Marrink, J. et al. (1991) Int. J. Cancer 49:368.
- Badcock, G. et al. (1999) Cancer Res. 59:4715.
- Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Product Datasheets
Citations for Human TRA-1-60(R) PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors
Authors: Breanna S. Borys, Tiffany Dang, Tania So, Leili Rohani, Tamas Revay, Tylor Walsh et al.
Stem Cell Research & Therapy
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Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors
Authors: Breanna S. Borys, Tania So, James Colter, Tiffany Dang, Erin L. Roberts, Tamas Revay et al.
Stem Cells Translational Medicine
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Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes
Authors: Paul W. Burridge, Elena Matsa, Praveen Shukla, Ziliang C. Lin, Jared M. Churko, Antje D. Ebert et al.
Nature Methods
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10^6 MDA-MB-231 cells were incubated for 30 minutes at 4C with 10 ul of Mouse TRA-1-60 PE-conjugated antibody (red), or control antibody (black). Blocking: 1% BSA. Unfixed cells.
