Human TOP2B Antibody

Catalog # Availability Size / Price Qty
AF6348
AF6348-SP
Detection of Human TOP2B by Western Blot.
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Human TOP2B Antibody Summary

Species Reactivity
Human
Specificity
Detects human TOP2B in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TOP2B
aa 1187-1621
Accession # Q02880
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Knockout Validated
TOP2B is specifically detected in HEK293 human embryonic kidney parental cell line but is not detectable in TOP2B knockout HEK293 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TOP2B antibody by Western Blot. View Larger

Detection of Human TOP2B by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human TOP2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6348) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TOP2B at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group1.

Knockout Validated Western Blot Shows Human TOP2B Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human TOP2B Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293 human embryonic kidney parental cell line and TOP2B knockout HEK293 cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human TOP2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6348) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TOP2B at approximately 245 kDa (as indicated) in the parental HEK293 cell line, but is not detectable in knockout HEK293 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TOP2B

TOP2B (DNA Topoisomerase II beta ) is a 180‑185 kDa member of the type IIA subfamily, topoisomerase family of molecules. It is ubiquitously expressed, and represents the larger of two known topoisomerases (the 2A/ alpha -form being 170 kDa in size). TOP2A is essential for cell division, while TOP2B is active postmitotically. In an ATP-dependent manner, homodimeric TOP2B relieves torsional stress created in DNA during transcription, or as a consequence of replication. TOP2B first induces cleavage of double-stranded DNA, creating a space that allows for the physical repositioning of chromatin and a reduction in tension. This is followed by closure and ligation of the cleaved ends to recreate the original DNA structure. Human TOP2B is 1626 amino acids (aa) in length. It contains an ATPase domain (aa 101‑201), an NES (aa 1034‑1044), more that 30 Ser/Thr phosphorylation sites and a C-terminal DTHCT region (aa 1508‑1611). There is one splice variant that shows a deletion of aa 24‑28. Over aa 1187‑1621, human TOP2B shares 91% aa identity with mouse TOP2B.

Long Name
DNA Topoisomerase II beta
Entrez Gene IDs
7155 (Human); 21974 (Mouse)
Alternate Names
antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; EC 5.99.1.3; TOP2B; top2beta; TOPIIB; topo II beta; topoisomerase (DNA) II beta (180kD); topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen

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