Human TLR9 Antibody

Catalog #: MAB36583 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB36583-100
MAB36583-SP

Detection of TLR9 in Human PBMCs by Flow Cytometry.

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All TLR9 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR9 Antibody Summary

Specificity

Detects human TLR9 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 26C593R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Antibody developed against the KLH-conjugated synthetic peptide corresponding to amino acids Asp250-Asn300 of human TLR9 isoform A.
Accession # Q9NR96

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of TLR9 antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of TLR9 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and (A) Mouse Anti-Human TLR9 Monoclonal Antibody (Catalog # MAB36583) or (B) Mouse IgG1 control antibody (Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR9

TLR9 (Toll receptor 9; also CD289) is a 145-150 kDa member of the Toll-like receptor family of molecules. It is expressed by colonic epithelium, CD123⁺ plasmacytoid dendritic cells, and transitional B cells, and responds to unmethylated DNA CpG motifs that contain either a GTCGTT sequence (in human), or a GACGTT sequence (in mouse). TLR9 is found in the ER and translocates to either the cell membrane or to lysosomes where it binds bacterial DNA. Precursor human TLR9 is a type I transmembrane protein 1032 amino acids (aa) in length. It possesses a 793 aa extracellular region that contains 26 LRRs (aa 26-818) plus a 193 aa cytoplasmic domain. The full-length 150 kDa form is suggested to be ligand-binding but nonsignaling. The active form is believed to be an 80 kDa cleavage product found in the endosome compartment. There are multiple splice forms. One contains a deletion of aa 2-16, a second possesses an alternate start site at Met58, while a third and fourth show alternative start sites aa 23 and 24 upstream of the standard site. Over aa 64-189, human TLR9 shares 76% aa identity with mouse TLR9.

Long Name

Toll-like Receptor 9

Entrez Gene IDs

54106 (Human); 81897 (Mouse); 338457 (Rat)

Alternate Names

CD289 antigen; CD289; TLR9; toll-like receptor 9