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Human TLR4 PerCP-conjugated Antibody

Catalog #: FAB6248C
1 Citation Datasheet / COA / SDS
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FAB6248C
Detection of TLR4 in Human Blood Monocytes by Flow Cytometry.
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Citations (1)
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Human TLR4 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TLR4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TLR1, 2, 3, 5, 7, 8, 9, 10, recombinant mouse (rm) TLR4, rmTLR6, rhTIRAP, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, rhST2, rhIL-18 R, rhIL-1 Rrp2, rhIL-18 R beta, rhSIGIRR, rhIL-1 RAPL1, rhIL-1 RAPL2, rhMD-1, rhMD-2, or rmRP105 is observed.
Source
Monoclonal Mouse IgG2A Clone # 610015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TLR4
Glu24-Lys631
Accession # O00206
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TLR4 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of TLR4 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TLR4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB6248C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR4

Toll-Like Receptor (TLR4) belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name
Toll-like Receptor 4
Entrez Gene IDs
7099 (Human); 21898 (Mouse); 29260 (Rat)
Alternate Names
ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

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Citation for Human TLR4 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Enhanced uropathogenic Escherichia coli-induced infection in uroepithelial cells by sugar through TLR-4 and JAK/STAT1 signaling pathways
    Authors: CH Ho, CK Fan, CC Wu, HJ Yu, HT Liu, KC Chen, SP Liu, PC Cheng
    J Microbiol Immunol Infect, 2019-06-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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