Human TLR4 PE-conjugated Antibody

Catalog #: FAB6248P
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB6248P

Detection of TLR4 in Human Blood Monocytes by Flow Cytometry.

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All TLR4 Antibodies

Product Details

Citations (8)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR4 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TLR1, 2, 3, 5, 7, 8, 9, 10, recombinant mouse (rm) TLR4, rmTLR6, rhTIRAP, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, rhST2, rhIL-18 R, rhIL-1 Rrp2, rhIL-18 R beta, rhSIGIRR, rhIL-1 RAPL1, rhIL-1 RAPL2, rhMD-1, rhMD-2, or rmRP105 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 610015

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TLR4
Glu24-Lys631
Accession # O00206

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TLR4 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TLR4 PE-conjugated Monoclonal Antibody (Catalog # FAB6248P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR4

Toll-Like Receptor (TLR4) belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name

Toll-like Receptor 4

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Alternate Names

ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TLR4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Antimicrobial Peptide LCN2 Inhibited Uropathogenic Escherichia coli Infection in Bladder Cells in a High-Glucose Environment through JAK/STAT Signaling Pathway
Authors: PC Chen, CH Ho, CK Fan, SP Liu, PC Cheng
International Journal of Molecular Sciences, 2022-12-12;23(24):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Phenotypic and functional features of innate and adaptive immunity as putative biomarkers for clinical status and leprosy reactions
Authors: J Campos de, MG Araújo, JG Alves Coel, V Peruhype-M, CC Alvares, M de Lima Mo, A Teixeira-C, OA Martins-Fi, MS Silva Araú
Microb. Pathog., 2018-09-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phagocytosis and Inflammation: Exploring the effects of the components of E-cigarette vapor on macrophages
Authors: MP Ween, JJ Whittall, R Hamon, PN Reynolds, SJ Hodge
Physiol Rep, 2017-08-01;5(16):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Profiling of the three circulating monocyte subpopulations in human obesity.
Authors: Devevre E, Renovato-Martins M, Clement K, Sautes-Fridman C, Cremer I, Poitou C
J Immunol, 2015-03-18;194(8):3917-23.
Species: Human
Sample Types: Whole Blood
Applications: Flow Cytometry
Serum amyloid A in the placenta and its role in trophoblast invasion.
Authors: Sandri S, Urban Borbely A, Fernandes I, de Oliveira E, Knebel F, Ruano R, Zugaib M, Filippin-Monteiro F, Bevilacqua E, Campa A
PLoS ONE, 2014-03-10;9(3):e90881.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Ex vivo cytokine release and pattern recognition receptor expression of subjects exposed to dampness: pilot study to assess the outcome of mould exposure to the innate immune system.
Authors: Punsmann S, Liebers V, Lotz A, Bruning T, Raulf M
PLoS ONE, 2013-12-10;8(12):e82734.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
J Allergy Clin Immunol, 2013-09-05;133(1):172-80.e1-10.
Species: Human
Sample Types: Whole Cells
Applications: Chip Cytometry
HPV infection alters vaginal microbiome through down-regulating host mucosal innate peptides used by Lactobacilli as amino acid sources
Authors: A Lebeau, D Bruyere, P Roncarati, P Peixoto, E Hervouet, G Cobraivill, B Taminiau, M Masson, C Gallego, G Mazzucchel, N Smargiasso, M Fleron, D Baiwir, E Hendrick, C Pilard, T Lerho, C Reynders, M Ancion, R Greimers, JC Twizere, G Daube, G Schlecht-L, F Bachelerie, JD Combes, P Melin, M Fillet, P Delvenne, P Hubert, M Herfs
Nature Communications, 2022-02-28;13(1):1076.